HCT116 whole cell lysate

Catalog No.

CLH00034

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Colorectal carcinoma cell targeting aromatherapy with Teucrium ramosissimum essential oil to sensitize TRAIL/Apo2L-induced HCT-116 cell death., PMID:38850792

Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer., PMID:37241967

Design and synthesis of Aza-boeravinone derivatives as potential novel topoisomerase I inhibitors., PMID:35338969

Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines., PMID:32916986

Redesigning the T-probe for mass spectrometry analysis of online lysis of non-adherent single cells., PMID:31519234

Time-dependent shotgun proteomics revealed distinct effects of an organoruthenium prodrug and its activation product on colon carcinoma cells., PMID:30106070

Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions., PMID:28528272

Whole gel processing procedure for GeLC-MS/MS based proteomics., PMID:23617947

Decitabine, a DNA methyltransferases inhibitor, induces cell cycle arrest at G2/M phase through p53-independent pathway in human cancer cells., PMID:23582784

Proteomic profiling in distinct cellular compartments of tumor cells reveals p53-dependent upregulation of S100A6 upon induction of telomere dysfunction., PMID:19834903

Quantitative protein network monitoring in response to DNA damage., PMID:18173236

Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein., PMID:15958392

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