HT29 whole cell lysate

Catalog No.

CLH00029

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer., PMID:37241967

Palmitoyl piperidinopiperidine, a novel derivative of 10‑hydroxy‑2‑decenoic acid, as a potent and selective anticancer agent against human colon carcinoma cell lines., PMID:33491745

Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines., PMID:32916986

BRAF protein immunoprecipitation, elution, and digestion from cell extract using a microfluidic mixer for mutant BRAF protein quantification by mass spectrometry., PMID:30604035

Differential proteome and cellular adhesion analyses of the probiotic bacterium Lactobacillus acidophilus NCFM grown on raffinose - an emerging prebiotic., PMID:26959526

Comparison of in vitro methods for carboxylesterase activity determination in immortalized cells representative of the intestine, liver and kidney., PMID:25979594

Vitamin B₆ activates p53 and elevates p21 gene expression in cancer cells and the mouse colon., PMID:24626782

Global phosphoproteome of HT-29 human colon adenocarcinoma cells., PMID:16083285

Microarray and proteomics analyses of human intestinal epithelial cells treated with the Aeromonas hydrophila cytotoxic enterotoxin., PMID:15845465

Aeromonas hydrophila cytotoxic enterotoxin activates mitogen-activated protein kinases and induces apoptosis in murine macrophages and human intestinal epithelial cells., PMID:15215244

Functional modulation of enterocytes by gram-positive and gram-negative microorganisms., PMID:15010363

Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells., PMID:10946324

A monoclonal antibody inhibits adhesion to fibronectin and vitronectin of a colon carcinoma cell line and recognizes the integrins alpha v beta 3, alpha v beta 5, and alpha v beta 6., PMID:7513610

Antibodies to normal human colon membranes: preparation, characterization and tissue distribution., PMID:2411694

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