Catalog No.
CLH00028
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Proteomic Signature of Extracellular Vesicles Associated with Colorectal Cancer., PMID:37241967
Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines., PMID:32916986
Impairment of electroneutral Na+ transport and associated downregulation of NHE3 contributes to the development of diarrhea following in vivo challenge with Brachyspira spp., PMID:31760765
Decreased electrogenic anionic secretory response in the porcine colon following in vivo challenge with Brachyspira spp. supports an altered mucin environment., PMID:30629469
Measuring oxygen levels in Caco-2 cultures., PMID:27774482
Inhibition of histone deacetylase 6 restores intestinal tight junction in hemorrhagic shock., PMID:27257709
Homogenous Phase Enrichment of Cysteine-Containing Peptides for Improved Proteome Coverage., PMID:26028250
Comparison of in vitro methods for carboxylesterase activity determination in immortalized cells representative of the intestine, liver and kidney., PMID:25979594
Vitamin B₆ activates p53 and elevates p21 gene expression in cancer cells and the mouse colon., PMID:24626782
Development of an enzymatic assay for the detection of neutralizing antibodies against therapeutic angiotensin-converting enzyme 2 (ACE2)., PMID:23298658
Inhibitory effect of calcium on non-heme iron absorption may be related to translocation of DMT-1 at the apical membrane of enterocytes., PMID:20597505
Disposition of flavonoids via enteric recycling: determination of the UDP-glucuronosyltransferase isoforms responsible for the metabolism of flavonoids in intact Caco-2 TC7 cells using siRNA., PMID:17927138
Heat shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells., PMID:14742538
Enteropathogenic Escherichia coli outer membrane proteins induce changes in cadherin junctions of Caco-2 cells through activation of PKCalpha., PMID:14738892
For research use only. Not for human or drug use.
