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HEK293 whole cell lysate

Catalog #:   CLH00025 Specific References (50) DATASHEET
Applications: WB
Overview

Catalog No.

CLH00025

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Data Image
References

A bacterial RING ubiquitin ligase triggering stepwise degradation of BRISC via TOLLIP-mediated selective autophagy manipulates host inflammatory response., PMID:40013521

A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies., PMID:39484553

Analysis of low-density lipoprotein receptor gene mutations in a family with familial hypercholesterolemia., PMID:39392848

Unravelling HDAC Selectivity for Erasing Acetyl Mark on Lys-5 of Histone H2B., PMID:38251898

Potent immunogenicity and neutralization of recombinant adeno-associated virus expressing the glycoprotein of severe fever with thrombocytopenia virus., PMID:38143087

Novel TPR::ROS1 Fusion Gene Activates MAPK, PI3K and JAK/STAT Signaling in an Infant-type Pediatric Glioma., PMID:36316040

Novel pathogenic ALG2 mutation causing congenital myasthenic syndrome: A case report., PMID:34980536

Molecular studies into cell biological role of Copine-4 in Retinal Ganglion Cells., PMID:34847148

Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins., PMID:34267198

Somatic frameshift mutation in PIK3CA causes CLOVES syndrome by provoking PI3K/AKT/mTOR pathway., PMID:34074347

Euodia pasteuriana Methanol Extract Exerts Anti-Inflammatory Effects by Targeting TAK1 in the AP-1 Signaling Pathway., PMID:33297427

Novel compound variants of the AR and MAP3K1 genes are related to the clinical heterogeneity of androgen insensitivity syndrome., PMID:32338288

Systematic Comparison of Strategies for the Enrichment of Lysosomes by Data Independent Acquisition., PMID:31738065

S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells., PMID:31472042

Suppression of Src and Syk in the NF-κB signaling pathway by Olea europaea methanol extract is leading to its anti-inflammatory effects., PMID:30710734

Altered expression of p63 isoforms and expansion of p63- and club cell secretory protein-positive epithelial cells in the lung as novel features of aging., PMID:30649915

Pathogenic variant in EPHB4 results in central conducting lymphatic anomaly., PMID:29905864

Selective Substrates and Activity-Based Probes for Imaging of the Human Constitutive 20S Proteasome in Cells and Blood Samples., PMID:29806773

Carbonyl reduction of NNK by recombinant human lung enzymes: identification of HSD17β12 as the reductase important in (R)-NNAL formation in human lung., PMID:29788210

ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity., PMID:29717462

Momordica charantia Inhibits Inflammatory Responses in Murine Macrophages via Suppression of TAK1., PMID:29463104

Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity., PMID:29018126

HEK293T cell lines defective for O-linked glycosylation., PMID:28654657

An avirulent Burkholderia pseudomallei ∆purM strain with atypical type B LPS: expansion of the toolkit for biosafe studies of melioidosis., PMID:28592242

Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions., PMID:28528272

CaMKII modulates sodium current in neurons from epileptic Scn2a mutant mice., PMID:28137877

DO-RIP-seq to quantify RNA binding sites transcriptome-wide., PMID:27840290

Pyrrole-Derivative of Chalcone, (E)-3-Phenyl-1-(2-Pyrrolyl)-2-Propenone, Inhibits Inflammatory Responses via Inhibition of Src, Syk, and TAK1 Kinase Activities., PMID:27469142

The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca2+ handling., PMID:27421120

Quantifying RNA binding sites transcriptome-wide using DO-RIP-seq., PMID:27742911

Zinc-Induced Polymerization of Killer-Cell Ig-like Receptor into Filaments Promotes Its Inhibitory Function at Cytotoxic Immunological Synapses., PMID:27058785

Altered Trafficking and Processing of GALC Mutants Correlates with Globoid Cell Leukodystrophy Severity., PMID:26865610

Identification of a novel mitochondrial interacting protein of C1QBP using subcellular fractionation coupled with CoIP-MS., PMID:26753982

Proteomic and bioinformatic analysis of a nuclear intrinsically disordered proteome., PMID:26376097

IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation., PMID:26208754

Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies., PMID:26126808

An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications., PMID:26049097

Dynamic imaging of pancreatic nuclear factor κB (NF-κB) activation in live mice using adeno-associated virus (AAV) infusion and bioluminescence., PMID:25802340

Characterization of Cav1.4 complexes (α11.4, β2, and α2δ4) in HEK293T cells and in the retina., PMID:25468907

Inhibitory role of phosphatidylinositol 4,5-bisphosphate on TMEM16A-encoded calcium-activated chloride channels in rat pulmonary artery., PMID:24834965

Vitamin B₆ activates p53 and elevates p21 gene expression in cancer cells and the mouse colon., PMID:24626782

Characterization of a monoclonal antibody capable of reliably quantifying expression of human Copper Transporter 1 (hCTR1)., PMID:24447817

A chemical tool for in vitro and in vivo precipitation of lysine methyltransferase G9a., PMID:24443078

Preparation of protein samples for mass spectrometry and N-terminal sequencing., PMID:24423264

Mesdc2 plays a key role in cell-surface expression of Lrp4 and postsynaptic specialization in myotubes., PMID:24140340

Characterization of a novel mutant KCNQ1 channel subunit lacking a large part of the C-terminal domain., PMID:24070608

Ankyrin-3 is a novel binding partner of the voltage-gated potassium channel Kv1.1 implicated in renal magnesium handling., PMID:23903368

[Recombinant adeno-associated virus type 8 mediated dual-luciferase gene expression in mouse]., PMID:23627024

Absolute quantification of cell-bound DNA aptamers during SELEX., PMID:23405949

In vitro and in vivo anti-inflammatory effect of Rhodomyrtus tomentosa methanol extract., PMID:23295168

Datasheet

Document Download

HEK293 whole cell lysate.pdf

 

$ 167
Product specifications
100 μg 167

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For research use only. Not for human or drug use.

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HEK293 whole cell lysate [CLH00025]
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