Catalog No.
CLH00024
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
S-palmitoylation coordinates the trafficking of ATG9A to mediate autophagy initiation., PMID:40394978
A Heterozygous LMF1 Gene Mutation (c.1523C>T), Combined With an LPL Gene Mutation (c.590G>A), Aggravates the Clinical Symptoms in Hypertriglyceridemia., PMID:35368694
Two Novel Mutations (G774A and A1685G) Causing Coagulation Factor XII Deficiency in a Patient with Acute Inferior Myocardial Infarction., PMID:34162575
Characteristics and possible mechanisms of 46, XY differences in sex development caused by novel compound variants in NR5A1 and MAP3K1., PMID:34112222
PEX6 Mutations in Peroxisomal Biogenesis Disorders: An Usher Syndrome Mimic., PMID:36249295
Novel compound variants of the AR and MAP3K1 genes are related to the clinical heterogeneity of androgen insensitivity syndrome., PMID:32338288
S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells., PMID:31472042
Molecularly imprinted polymers synthesized via template immobilization on fumed silica nanoparticles for the enrichment of phosphopeptides., PMID:29024117
Low-bias phosphopeptide enrichment from scarce samples using plastic antibodies., PMID:26126808
Comparison of in vitro methods for carboxylesterase activity determination in immortalized cells representative of the intestine, liver and kidney., PMID:25979594
Unexpected lack of specificity of a rabbit polyclonal TAP-L (ABCB9) antibody., PMID:27335633
Characterization of a novel mutant KCNQ1 channel subunit lacking a large part of the C-terminal domain., PMID:24070608
Problems encountered in bicistronic IRES-GFP expression vectors employed in functional analyses of GC-induced genes., PMID:23076521
[Study of neutralization antibodie induced by DNA vaccine of HCV envelope protein 2 in mice]., PMID:21695897
Role for protein kinase C delta in the functional activity of human UGT1A6: implications for drug-drug interactions between PKC inhibitors and UGT1A6., PMID:20196639
[Molecular mechanisms of Glanzmann thrombasthenia caused by alpha II b L721R and Q860X compound heterozygous mutation]., PMID:19175981
[Molecular mechanisms of Glanzmann thrombasthenia caused by alphaII b P126H mutation: an in vitro experiment]., PMID:19040019
Epidermal growth factor receptor double activating mutations involving both exons 19 and 21 exist in Chinese non-small cell lung cancer patients., PMID:17537621
For research use only. Not for human or drug use.
