Catalog No.
CLH00013
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival., PMID:37883333
Simplified Rapid Enrichment of CTCs and Selective Recognition Prereduction Enable a Homogeneous ICP-MS Liquid Biopsy Strategy of Lung Cancer., PMID:37705297
Screening for inhibitory effects of crude drugs on furin-like enzymatic activities., PMID:33928494
Identification, localization and expression of NHE isoforms in the alveolar epithelial cells., PMID:33882062
Alveolar epithelial inter-alpha-trypsin inhibitor heavy chain 4 deficiency associated with senescence-regulated apoptosis by air pollution., PMID:33735794
Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines., PMID:32916986
A microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease amplification for selective and sensitive detection of miRNAs in cancer cells., PMID:32456922
Parallel Channels-Multidimensional Protein Identification Technology., PMID:32310654
Cell surface processing of the P1 adhesin of Mycoplasma pneumoniae identifies novel domains that bind host molecules., PMID:32286369
Irradiation increases the immunogenicity of lung cancer cells and irradiation-based tumor cell vaccine elicits tumor-specific T cell responses in vivo., PMID:31190880
Elucidating differential nano-bio interactions of multi-walled andsingle-walled carbon nanotubes using subcellular proteomics., PMID:29688820
Polymeric Nano-Encapsulation of Curcumin Enhances its Anti-Cancer Activity in Breast (MDA-MB231) and Lung (A549) Cancer Cells Through Reduction in Expression of HIF-1α and Nuclear p65 (Rel A)., PMID:29065834
An avirulent Burkholderia pseudomallei ∆purM strain with atypical type B LPS: expansion of the toolkit for biosafe studies of melioidosis., PMID:28592242
Transcriptional organization of pneumococcal psrP-secY2A2 and impact of GtfA and GtfB deletion on PsrP-associated virulence properties., PMID:28408270
Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines., PMID:26363641
In vitro analysis of transport and metabolism of 4'-thiothymidine in human tumor cells., PMID:25659855
Pre-B cell colony enhancing factor induces Nampt-dependent translocation of the insulin receptor out of lipid microdomains in A549 lung epithelial cells., PMID:25516545
Identification of a bacteriolysis-associated virulence factor against lung epithelial cells in Pseudomonas aeruginosa PAO-1 cell lysate., PMID:25152458
A comprehensive proteomic view of responses of A549 type II alveolar epithelial cells to human respiratory syncytial virus infection., PMID:25106423
Metformin inhibits heme oxygenase-1 expression in cancer cells through inactivation of Raf-ERK-Nrf2 signaling and AMPK-independent pathways., PMID:23707609
Multiple reaction monitoring of multiple low-abundance transcription factors in whole lung cancer cell lysates., PMID:23586733
Decitabine, a DNA methyltransferases inhibitor, induces cell cycle arrest at G2/M phase through p53-independent pathway in human cancer cells., PMID:23582784
Proteomic profiling in distinct cellular compartments of tumor cells reveals p53-dependent upregulation of S100A6 upon induction of telomere dysfunction., PMID:19834903
Biochemical interactions among intercellular adhesion molecules expressed by airway epithelial cells., PMID:16546130
Staphylococcus aureus deficient in lipidation of prelipoproteins is attenuated in growth and immune activation., PMID:15784587
Induction of nitric oxide release from the human alveolar epithelial cell line A549: an in vitro correlate of innate immune response to Mycobacterium tuberculosis., PMID:15196216
Dendritic cells efficiently acquire and present antigen derived from lung cancer cells and induce antigen-specific T-cell responses., PMID:12835918
Respiratory syncytial virus infection of human alveolar epithelial cells enhances interferon regulatory factor 1 and interleukin-1beta-converting enzyme gene expression but does not cause apoptosis., PMID:9557748
For research use only. Not for human or drug use.
