Catalog No.
CLH00003
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Detection of low-copy proteins in proteomic studies: issues and solutions., PMID:39324198
Glycan node profiling of soluble and membrane glycoproteins in whole cell lysates., PMID:37699507
Design and synthesis of Aza-boeravinone derivatives as potential novel topoisomerase I inhibitors., PMID:35338969
Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture., PMID:34035320
Fluorescence Polarization-Based Measurement of Protein-Ligand Interaction in Fungal Cell Lysates., PMID:33484500
S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T cells., PMID:31472042
Antigen-loaded dendritic cells triggers a specific cytotoxic T lymphocytes immune response against hepatocellular carcinoma: in vitro study., PMID:30368725
Proteomic Comparison of Various Hepatic Cell Cultures for Preclinical Safety Pharmacology., PMID:29635369
Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells., PMID:28555032
Interrogating Endogenous Protein Phosphatase Activity with Rationally Designed Chemosensors., PMID:26580981
Vitamin B₆ activates p53 and elevates p21 gene expression in cancer cells and the mouse colon., PMID:24626782
Metformin inhibits heme oxygenase-1 expression in cancer cells through inactivation of Raf-ERK-Nrf2 signaling and AMPK-independent pathways., PMID:23707609
Effect of knockdown of ezrin, radixin, and moesin on P-glycoprotein function in HepG2 cells., PMID:21837648
Analysis of arsenic metabolites in HepG2 and AS3MT-transfected cells., PMID:21537954
Development and characterisation of an assay for furin activity., PMID:21112328
Schistosoma mansoni soluble egg antigens enhance HCV replication in mammalian cells., PMID:20440060
[Real time ex vivo detection and dynamic monitoring of in vivo expression of secreted luciferase gene injected by hydrodynamic method]., PMID:20112701
Two-dimensional blue native/SDS-PAGE analysis reveals heat shock protein chaperone machinery involved in hepatitis B virus production in HepG2.2.15 cells., PMID:18984579
Absolute SILAC for accurate quantitation of proteins in complex mixtures down to the attomole level., PMID:18271523
Establishment of subcellular fractionation techniques to monitor the intracellular fate of polymer therapeutics II. Identification of endosomal and lysosomal compartments in HepG2 cells combining single-step subcellular fractionation with fluorescent imaging., PMID:17365272
Investigation of differentially expressed proteins due to the inhibitory effects of berberine in human liver cancer cell line HepG2., PMID:16880943
Differential protein expression of the inhibitory effects of a standardized extract from Scutellariae radix in liver cancer cell lines using liquid chromatography and tandem mass spectrometry., PMID:15631501
Cytotoxic effects of pyocin S2 produced by Pseudomonas aeruginosa on the growth of three human cell lines., PMID:15213746
Significant correlation between expression level of HSP gp96 and progression of hepatitis B virus induced diseases., PMID:15069714
Lack of tyrosine nitration by hypochlorous acid in the presence of physiological concentrations of nitrite. Implications for the role of nitryl chloride in tyrosine nitration in vivo., PMID:12475975
Transforming growth factor beta targeted inactivation of cyclin E:cyclin-dependent kinase 2 (Cdk2) complexes by inhibition of Cdk2 activating kinase activity., PMID:10611320
Association of insulin-degrading enzyme with a 70 kDa cytosolic protein in hepatoma cells., PMID:8870662
RNA-binding proteins that specifically recognize the selenocysteine insertion sequence of human cellular glutathione peroxidase mRNA., PMID:8530473
Micropreparative immobilized pH gradient two-dimensional electrophoresis in combination with protein microsequencing for the analysis of human liver proteins., PMID:8586070
Differential regulation of two glucose transporters by chronic growth hormone treatment of cultured 3T3-F442A adipose cells., PMID:2254335
Detection of multiple forms of human ceruloplasmin. A novel Mr 200,000 form., PMID:2154449
Synthesis of complement component C5 by human B and T lymphoblastoid cell lines., PMID:1969382
For research use only. Not for human or drug use.
