EndoS (YXX05201)

Catalog No.

YXX05201

Description

Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.

Biological activity

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.

Expression system

E. coli

Species

Streptococcus pyogenes

Protein length

EndoS is cloned from Streptococcus pyogenes and expressed in E.coli.

Predicted molecular weight

109.35 kDa

Nature

Recombinant

Target

Endo S

Concentration

40000U/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Applications

Expression Systems,Proteomics,Glycan Sequencing,Recombinant Glycoprotein Expression,Glycoprotein Analysis

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% glycerol

Experimental Procedure

Protocol

Digestion reaction condition

Reagent	Volume
Native IgG concentration(μg/μl)	C
Native IgG volume (μl)	V
Native IgG quality (μg)	100
Endo S(U)	20
Endo S(μl)	0.5
10X buffer(μl）	200
ddH2O	(200*10)-0.5-V
Reaction system(μl)	200*10

Incubate reaction at 37°C for 1 hour.

 

1X Buffer
5 mM CaCl₂
50 mM sodium acetate
(pH 5.5 @ 25°C)

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Document Download

EndoS.pdf