M-MLV

Catalog No.

YVV07601

Description

M-MLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.

Expression system

E.coli

Species

MoMLV

Protein length

M-MLV is cloned from MoMLV and expressed in E.coli.

Predicted molecular weight

75.85 kDa

Nature

Recombinant

Target

M-MLV

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Applications

cDNA Synthesis,Reverse Transcription (cDNA Synthesis),RT-PCR & cDNA Synthesis,RT-qPCR,RT-PCR and cDNA Synthesis,PCR

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Reconstitution

Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

First Report of Chilli Yellow Ringspot Virus Infecting Tomato (Solanum lycopersicum) in China., PMID:40208639

Development of a prime editor with improved editing efficiency in Arabidopsis., PMID:39681408

Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems., PMID:39061809

Structural basis for pegRNA-guided reverse transcription by a prime editor., PMID:38811740

Enhancing prime editor activity by directed protein evolution in yeast., PMID:38453904

Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system., PMID:38411149

An Open One-Step RT-qPCR for SARS-CoV-2 detection., PMID:38271448

An optimized prime editing system for efficient modification of the pig genome., PMID:37505431

One-step Reverse Transcription-LoopMediated Isothermal Amplification (RT-LAMP) for closed-tube colorimetric detection of banana bract mosaic virus in Banana (Musa spp.)., PMID:37064005

Prime editing with genuine Cas9 nickases minimizes unwanted indels., PMID:36997524

Addition of the T5 exonuclease increases the prime editing efficiency in plants., PMID:36958601

A truncated reverse transcriptase enhances prime editing by split AAV vectors., PMID:35808824

An Open One-Step RT-qPCR for SARS-CoV-2 detection., PMID:34909786

A low-cost and open-source protocol to produce key enzymes for molecular detection assays., PMID:34766029

Homebrew reagents for low cost RT-LAMP., PMID:34013302

First report of wild tomato mosaic virus infecting chilli pepper in China., PMID:33926200

Simple, Inexpensive RNA Isolation and One-Step RT-qPCR Methods for SARS-CoV-2 Detection and General Use., PMID:33905620

First report of Sugarcane Streak Mosaic Virus (SCSMV) infecting sugarcane in Côte d'Ivoire., PMID:32840438

First report of vicia cryptic virus M infecting cowpea (Vigna unguiculata) in China., PMID:32734848

Fluorescent Tricyclic Cytidine Analogues as Substrates for Retroviral Reverse Transcriptases., PMID:32378814

Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus., PMID:31694951

Expression of SOCS1 and SOCS3 Genes in Interferon-Treated and Direct-Acting Antiviral Drugs-Treated Hepatitis C Patients., PMID:29920131

Characterization of interaction between Trim28 and YY1 in silencing proviral DNA of Moloney murine leukemia virus., PMID:29407374

Arm-specific cleavage and mutation during reverse transcription of 2΄,5΄-branched RNA by Moloney murine leukemia virus reverse transcriptase., PMID:28160599

One-Pot Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) for Detecting MERS-CoV., PMID:28119682

Structural and sequencing analysis of local target DNA recognition by MLV integrase., PMID:25969444

First Report of Fig mosaic virus Infecting Common Fig (Ficus carica) in China., PMID:30699710

First Report of Cherry mottle leaf virus Infecting Cherry in China., PMID:30708833

Gene expression profiling of immune responsive and fibrosis genes in hepatitis C virus infected patients., PMID:24811215

The Nef-like effect of murine leukemia virus glycosylated gag on HIV-1 infectivity is mediated by its cytoplasmic domain and depends on the AP-2 adaptor complex., PMID:24403584

Patch cloning method for multiple site-directed and saturation mutagenesis., PMID:24164925

BDNF signaling is necessary for the antidepressant-like effect of naringenin., PMID:24121063

Knockdown of NOB1 expression by RNAi inhibits cellular proliferation and migration in human gliomas., PMID:23911301

Neonatal expression of amh, sox9 and sf-1 mRNA in Caiman latirostris and effects of in ovo exposure to endocrine disrupting chemicals., PMID:23747749

A M-MLV reverse transcriptase with reduced RNaseH activity allows greater sensitivity of gene expression detection in formalin fixed and paraffin embedded prostate cancer samples., PMID:23739432

Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue., PMID:23680332

HGV-HCV/HBV co-infection in India: A pilot study., PMID:23559765

Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for rapid detection of HDV genotype 1., PMID:23252739

Application of reverse transcription-PCR and real-time PCR in nanotoxicity research., PMID:22975959

Novel real-time simultaneous amplification and testing method to accurately and rapidly detect Mycobacterium tuberculosis complex., PMID:22205804

Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification., PMID:22185375

First Report of Pear blister canker viroid in Pear Trees in Argentina., PMID:30731718

Inhibition of HIV-1 and M-MLV reverse transcriptases by a major polyphenol (3,4,5 tri-O-galloylquinic acid) present in the leaves of the South African resurrection plant, Myrothamnus flabellifolia., PMID:21443452

Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA., PMID:21085668

Interaction of aurintricarboxylic acid (ATA) with four nucleic acid binding proteins DNase I, RNase A, reverse transcriptase and Taq polymerase., PMID:19836295

Decontamination of polymerase chain reaction reagents using DEAE-cellulose., PMID:19523915

TRIM28 mediates primer binding site-targeted silencing of murine leukemia virus in embryonic cells., PMID:17923087

First Report of Tobacco ringspot virus in Pumpkin (Cucurbita pepo) in Illinois., PMID:30780956

Tumor model-specific proviral insertional mutagenesis of the Fos/Jdp2/Batf locus., PMID:15913695

Development of a one tube-one step RT-PCR protocol for the detection of seven viroids in four genera: Apscaviroid, Hostuviroid, Pelamoviroid and Pospiviroid., PMID:15350729

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