Anti-NSUN2 Polyclonal Antibody (PHG19801)

specific references (0) DATASHEET

Host species：Rabbit

Isotype：IgG

Applications：ELISA, IHC, WB

Accession：Q08J23

Overview

Catalog No.

PHG19801

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NSUN2 (His415-Arg767).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Misu,NSUN2,hTrm4,RNA cytosine C(5)-methyltransferase NSUN2,TRM4,NOL1/NOP2/Sun domain family member 2,tRNA methyltransferase 4 homolog,mRNA cytosine C(5)-methyltransferase,SAKI,Substrate of AIM1/Aurora kinase B,Myc-induced SUN domain-containing protein,tRNA cytosine C(5)-methyltransferase

Purification

Purified by antigen affinity column.

Accession

Q08J23

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image

Western blot

Various lysates were subjected to SDS PAGE followed by western blot with NSUN2 antibody (PHG19801) at 0.68μg/ml.

Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: MDA-MB-435S cell lysate

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 86,59 kDa
Observed MW: 100,59 kDa
Immunocytochemistry/ Immunofluorescence

NSUN2 in HepG2 Cell Line.
The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with NSUN2 (PHG19801) at 13.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
Western Blot

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NSUN2 antibody (PHG19801) at 1 µg/ml.

Lane 1: Recombinant Protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 41 kDa
Observed MW: 41 kDa

References

Datasheet

Document Download

Anti-NSUN2 Polyclonal Antibody.pdf

$ 170

Product specifications

50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here