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Anti-NSUN2 Polyclonal Antibody (PHG19801)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:Q08J23
Overview

Catalog No.

PHG19801

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NSUN2 (His415-Arg767).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Misu,NSUN2,hTrm4,RNA cytosine C(5)-methyltransferase NSUN2,TRM4,NOL1/NOP2/Sun domain family member 2,tRNA methyltransferase 4 homolog,mRNA cytosine C(5)-methyltransferase,SAKI,Substrate of AIM1/Aurora kinase B,Myc-induced SUN domain-containing protein,tRNA cytosine C(5)-methyltransferase

Purification

Purified by antigen affinity column.

Accession

Q08J23

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with NSUN2 antibody (PHG19801) at 0.68μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: SH-SY5Y cell lysate
    Lane 4: MDA-MB-435S cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 86,59 kDa
    Observed MW: 100,59 kDa
  • Immunocytochemistry/ Immunofluorescence
    NSUN2 in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with NSUN2 (PHG19801) at 13.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-NSUN2 Polyclonal Antibody [PHG19801]
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