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Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody (PHD75801)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P27986
Overview

Catalog No.

PHD75801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIK3R1/PI3-kinase p85-alpha (Trp624-Val718).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PI3-kinase subunit p85-alpha,Phosphatidylinositol 3-kinase regulatory subunit alpha,PtdIns-3-kinase regulatory subunit alpha,GRB1,PI3K regulatory subunit alpha,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha,PtdIns-3-kinase regulatory subunit p85-alpha,PIK3R1,PI3-kinase regulatory subunit alpha

Purification

Purified by antigen affinity column.

Accession

P27986

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PI3-kinase p85-alpha antibody (PHD75801) at 1μg/ml.

    Lane 1: NIH3T3 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: N2A cell lysate
    Lane 4: A549 cell lysate
    Lane 5: PC 12 cell lysate
    Lane 6: HepG2 cell lysate
    Lane 7: MCF-7 cell lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse muscle lysate
    Lane 10: Mouse kidney lysate
    Lane 11: Mouse heart lysate
    Lane 12: Rat brain lysate
    Lane 13: Rat muscle lysate
    Lane 14: Rat kidney lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 85 kDa
    Observed MW: 85 kDa
  • Immunocytochemistry/ Immunofluorescence
    PIK3R1 / PI3-kinase p85-alpha in A549 Cell Line.
    The A549 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PIK3R1 / PI3-kinase p85-alpha (PHD75801) at 7.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-PIK3R1/PI3-kinase p85-alpha Polyclonal Antibody [PHD75801]
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