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Anti-ATP6AP2 Polyclonal Antibody (PHB30701)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:O75787
Overview

Catalog No.

PHB30701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP6AP2 (Asn17-Glu302).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Renin/prorenin receptor,ER-localized type I transmembrane adapter,ATPase H(+)-transporting lysosomal accessory protein 2,CAPER,ATP6M8-9,Embryonic liver differentiation factor 10,ATP6AP2,ATPase H(+)-transporting lysosomal-interacting protein 2,V-ATPase M8.9 subunit,ATP6IP2,Renin receptor,N14F,ELDF10,Vacuolar ATP synthase membrane sector-associated protein M8-9

Purification

Purified by antigen affinity column.

Accession

O75787

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATP6AP2 antibody (PHB30701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 34 kDa
    Observed MW: 34 kDa
  • Immunocytochemistry/ Immunofluorescence
    ATP6AP2 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ATP6AP2 (PHB30701) at 12.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-ATP6AP2 Polyclonal Antibody [PHB30701]
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