Anti-ARNTL Polyclonal Antibody (PHA09301)

specific references (0) DATASHEET

Host species：Rabbit

Isotype：IgG

Applications：ELISA, IHC, WB

Accession：O00327

Overview

Catalog No.

PHA09301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARNTL (Glu48-Leu320).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Basic-helix-loop-helix-PAS protein MOP3,ARNTL,Member of PAS protein 3,PAS domain-containing protein 3,BMAL1,Aryl hydrocarbon receptor nuclear translocator-like protein 1,BHLHE5,Class E basic helix-loop-helix protein 5,bHLHe5,MOP3,Brain and muscle ARNT-like 1,bHLH-PAS protein JAP3,PASD3

Purification

Purified by antigen affinity column.

Accession

O00327

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image

WESTERN BLOT

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ARNTL antibody (PHA09301) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 33 kDa
Observed MW: 33 kDa
Immunocytochemistry/ Immunofluorescence

ARNTL in NIH3T3 Cell Line.
The NIH3T3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ARNTL (PHA09301) at 13.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
Immunocytochemistry/ Immunofluorescence

ARNTL in NIH3T3 Cell Line.
The NIH3T3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ARNTL (PHA09301) at 13.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).

References

Datasheet

Document Download

Anti-ARNTL Polyclonal Antibody.pdf

$ 170

Product specifications

50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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