Catalog No.
YVV02002
Expression system
E. coli
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Ser6799-Asn7096
Predicted molecular weight
61 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P0DTD1
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
2'-O-methyltransferase, Non-structural protein 16, nsp16, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein
SARS-CoV-2 nsp16 is regulated by host E3 ubiquitin ligases, UBR5 and MARCHF7., PMID:40358464
Transfer learning towards predicting viral missense mutations: A case study on SARS-CoV-2., PMID:40352476
Structural insights into the assembly and regulation of 2'-O RNA methylation by SARS-CoV-2 nsp16/nsp10., PMID:40220753
A live attenuated SARS-CoV-2 vaccine constructed by dual inactivation of NSP16 and ORF3a., PMID:40132472
Screening of RNA methyltransferase NSP16 inhibitors against SARS-CoV-2 coronavirus and study of related mechanisms., PMID:40092096
SARS-CoV-2 enhances complement-mediated endothelial injury via the suppression of membrane complement regulatory proteins., PMID:39945674
Emergence of SARS-CoV-2 subgenomic RNAs that enhance viral fitness and immune evasion., PMID:39836705
Dynamic Combinatorial Chemistry Unveils Nsp10 Inhibitors with Antiviral Potential Against SARS-CoV-2., PMID:39676060
Cannabinoid-Inspired Inhibitors of the SARS-CoV-2 Coronavirus 2'-O-Methyltransferase (2'-O-MTase) Non-Structural Protein (Nsp10-16)., PMID:39519722
Identification of adenosine analogues as nsp14 N7‑methyltransferase inhibitors for treating coronaviruses infection., PMID:39490138
Structural Basis for Inhibition of the SARS-CoV-2 nsp16 by Substrate-Based Dual Site Inhibitors., PMID:39258386
SARS-CoV-2 NSP16 promotes IL-6 production by regulating the stabilization of HIF-1α., PMID:39251053
The Chameleon Strategy-A Recipe for Effective Ligand Screening for Viral Targets Based on Four Novel Structure-Binding Strength Indices., PMID:39066235
Structural and functional insights into the 2'-O-methyltransferase of SARS-CoV-2., PMID:38969340
Progressive Evolutionary Dynamics of Gene-Specific ω Led to the Emergence of Novel SARS-CoV-2 Strains Having Super-Infectivity and Virulence with Vaccine Neutralization., PMID:38928018
Natural evidence of coronaviral 2'-O-methyltransferase activity affecting viral pathogenesis via improved substrate RNA binding., PMID:38811528
Structure-Based Virtual Screening for Methyltransferase Inhibitors of SARS-CoV-2 nsp14 and nsp16., PMID:38792173
Cheminformatics approach to identify andrographolide derivatives as dual inhibitors of methyltransferases (nsp14 and nsp16) of SARS-CoV-2., PMID:38684706
Native Mass Spectrometry Dissects the Structural Dynamics of an Allosteric Heterodimer of SARS-CoV-2 Nonstructural Proteins., PMID:38535992
Despite the odds: formation of the SARS-CoV-2 methylation complex., PMID:38499483
AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor., PMID:38467836
In silico Evaluation of NO-Sartans against SARS-CoV-2., PMID:38445698
New insights into complex formation by SARS-CoV-2 nsp10 and nsp14., PMID:38422227
Reconstitution of RNA cap methylation reveals different features of SARS-CoV-2 and SARS-CoV methyltransferases., PMID:38285434
5'-cap RNA/SAM mimetic conjugates as bisubstrate inhibitors of viral RNA cap 2'-O-methyltransferases., PMID:38199140
Emerging variants of SARS-CoV-2 NSP10 highlight strong functional conservation of its binding to two non-structural proteins, NSP14 and NSP16., PMID:38127066
A repository of COVID-19 related molecular dynamics simulations and utilisation in the context of nsp10-nsp16 antivirals., PMID:37976980
Tonsils are major sites of persistence of SARS-CoV-2 in children., PMID:37737615
Potent multi-target natural inhibitors against SARS-CoV-2 from medicinal plants of the Himalaya: a discovery from hybrid machine learning, chemoinformatics, and simulation assisted screening., PMID:37732349
Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets., PMID:37704626
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering., PMID:37686452
Coronavirus 2'-O-methyltransferase: A promising therapeutic target., PMID:37634741
Targeting SARS-CoV-2 Non-Structural Proteins., PMID:37629182
Structural-Based Virtual Screening of FDA-Approved Drugs Repository for NSP16 Inhibitors, Essential for SARS-COV-2 Invasion Into Host Cells: Elucidation From MM/PBSA Calculation., PMID:37533429
AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor., PMID:37398436
Integrated molecular and quantum mechanical approach to identify novel potent natural bioactive compound against 2'-O-methyltransferase (nsp16) of SARS-CoV-2., PMID:37129206
A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases., PMID:37060263
Design and synthesis of naturally-inspired SARS-CoV-2 inhibitors., PMID:36970153
Global landscape of SARS-CoV-2 mutations and conserved regions., PMID:36841805
Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization., PMID:36793212
Coronavirus Inhibitors Targeting nsp16., PMID:36770656
SS148 and WZ16 inhibit the activities of nsp10-nsp16 complexes from all seven human pathogenic coronaviruses., PMID:36764586
Targeting viral proteins for restraining SARS-CoV-2: focusing lens on viral proteins beyond spike for discovering new drug targets., PMID:36723288
SARS-CoV-2 Uses Nonstructural Protein 16 To Evade Restriction by IFIT1 and IFIT3., PMID:36722972
3-(Adenosylthio)benzoic Acid Derivatives as SARS-CoV-2 Nsp14 Methyltransferase Inhibitors., PMID:36677825
Analysis of critical protein-protein interactions of SARS-CoV-2 capping and proofreading molecular machineries towards designing dual target inhibitory peptides., PMID:36611052
Computational Analysis of SAM Analogs as Methyltransferase Inhibitors of nsp16/nsp10 Complex from SARS-CoV-2., PMID:36430451
Identification and characterization of alternative sites and molecular probes for SARS-CoV-2 target proteins., PMID:36385993
Molecular Dynamics Simulations to Decipher the Role of Phosphorylation of SARS-CoV-2 Nonstructural Proteins (nsps) in Viral Replication., PMID:36366534
Nsp16 shields SARS-CoV-2 from efficient MDA5 sensing and IFIT1-mediated restriction., PMID:36285486
For research use only. Not for human or drug use.
