Catalog No.
YHK06201
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Ser469-Thr572
Predicted molecular weight
38.50 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q86YW0
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1, PLCZ1, Testis-development protein NYD-SP27, Phospholipase C-zeta-1, Phosphoinositide phospholipase C-zeta-1, PLC-zeta-1
Structural Implications of H233L and H398P Mutations in Phospholipase Cζ: A Full-Atom Molecular Dynamics Study on Infertility-Associated Dysfunctions., PMID:40429849
Genomic Insights into Fertilization: Tracing PLCZ1 Orthologs Across Amphibian Lineages., PMID:40106576
Roles of the EF-hand domain of PLCZ1 in establishing species-specific Ca2+ oscillations in mouse and rat fertilization†., PMID:39893624
Artificial oocyte activation improves ICSI outcomes following unexplained fertilization abnormalities., PMID:39241689
Novel PLCZ1 compound heterozygous mutations indicate gene dosage effect involved in total fertilisation failure after ICSI., PMID:39042720
The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice., PMID:38670547
Molecular Mechanism of Oocyte Activation in Mammals: Past, Present, and Future Directions., PMID:38540777
Microfluidic sperm sorting selects a subpopulation of high-quality sperm with a higher potential for fertilization., PMID:38461455
Novel PLCZ1 mutation caused polyspermy during in vitro fertilization., PMID:38445955
Phospholipase C Zeta in Human Spermatozoa: A Systematic Review on Current Development and Clinical Application., PMID:38279344
Expression of PLCZ1 mRNA in spermatozoa of Criollo and European bulls in a low-input system., PMID:38213766
Defects in phospholipase C zeta cause polyspermy and low fertilization after conventional IVF: not just ICSI failure., PMID:38048167
RNA sequencing profiles reveals progressively reduced spermatogenesis with progression in adult cryptorchidism., PMID:38027210
Genetic mechanisms of fertilization failure and early embryonic arrest: a comprehensive review., PMID:37758324
Cryopreservation of equine spermatozoa reduces plasma membrane integrity and phospholipase C zeta 1 content as associated with oocyte activation., PMID:37608516
Avian sperm-borne RNAs: optimisation of a new isolation protocol., PMID:37266980
Cryostress induces fragmentation and alters the abundance of sperm transcripts associated with fertilizing competence and reproductive processes in buffalo., PMID:37079096
Total fertilization failure after ICSI: insights into pathophysiology, diagnosis, and management through artificial oocyte activation., PMID:36977357
Assisted oocyte activation does not overcome recurrent embryo developmental problems., PMID:36931261
Plcz1 Deficiency Decreased Fertility in Male Mice Which Is Associated with Sperm Quality Decline and Abnormal Cytoskeleton in Epididymis., PMID:36613757
Novel variants in ACTL7A and PLCZ1 are associated with male infertility and total fertilization failure., PMID:36593593
Gene mutations associated with fertilization failure after in vitro fertilization/intracytoplasmic sperm injection., PMID:36589837
A novel homozygous mutation in ACTL7A leads to male infertility., PMID:36574082
Mutations in PLCZ1 induce male infertility associated with polyspermy and fertilization failure., PMID:36529831
Profiling the male germline genome to unravel its reproductive potential., PMID:36379263
Actl7a deficiency in mice leads to male infertility and fertilization failure., PMID:35921706
Microfluidic chips as a method for sperm selection improve fertilization rate in couples with fertilization failure., PMID:35650258
Inositol-1,4,5-Trisphosphate Receptor-1 and -3 and Ryanodine Receptor-3 May Increase Ooplasmic Ca2+ During Quail Egg Activation., PMID:35528380
SPERM FACTORS AND EGG ACTIVATION: The structure and function relationship of sperm PLCZ1., PMID:35521907
SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1., PMID:35521902
SPERM FACTORS AND EGG ACTIVATION: Phospholipase C zeta (PLCZ1) and the clinical diagnosis of oocyte activation deficiency., PMID:35312629
High Resolution Proteomic Analysis of Subcellular Fractionated Boar Spermatozoa Provides Comprehensive Insights Into Perinuclear Theca-Residing Proteins., PMID:35252197
SPERM FACTORS AND EGG ACTIVATION: The phenotype of PLCZ1-deficient mice., PMID:35239501
Omics and Male Infertility: Highlighting the Application of Transcriptomic Data., PMID:35207567
SPERM FACTORS AND EGG ACTIVATION: Fertilization failure after human ICSI and the clinical potential of PLCZ1., PMID:35038316
Antigen unmasking does not improve the visualization of phospholipase C zeta in human spermatozoa., PMID:34893574
Deletion of TRPV3 and CaV3.2 T-type channels in mice undermines fertility and Ca2+ homeostasis in oocytes and eggs., PMID:34313315
The paternal toolbox for embryo development and health., PMID:34191013
Novel DNAH17 mutations associated with fertilization failures after ICSI., PMID:34126833
Single-center thorough evaluation and targeted treatment of globozoospermic men., PMID:33877510
Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice., PMID:33626338
Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains., PMID:33208563
A new NLRP5 mutation causes female infertility and total fertilization failure., PMID:33073652
Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model., PMID:32898251
Novel mutations in the PLCZ1 gene associated with human low or failed fertilization., PMID:32840018
Identification and treatment of men with phospholipase Cζ-defective spermatozoa., PMID:32712020
Use of phospholipase C zeta analysis to identify candidates for artificial oocyte activation: a case series of clinical pregnancies and a proposed algorithm for patient management., PMID:32622408
Birth of offspring from spermatid or somatic cell by co-injection of PLCζ-cRNA., PMID:32585638
The soluble sperm factor that activates the egg: PLCzeta and beyond., PMID:32485666
Phospholipase C zeta profiles are indicative of optimal sperm parameters and fertilisation success in patients undergoing fertility treatment., PMID:32298520
For research use only. Not for human or drug use.
