Catalog No.
YHK03901
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Leu216
Predicted molecular weight
25.96 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9BU89
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Deoxyhypusine hydroxylase, hDOHH, DOHH, HLRC1, Deoxyhypusine dioxygenase, HEAT-like repeat-containing protein 1, Deoxyhypusine monooxygenase
Yeast reconstituted translation assays for analysis of eIF5A function., PMID:40382135
Genetic analyses of Myc and hypusine circuits in tumorigenesis., PMID:40382132
Development of a reliable, sensitive, and convenient assay for the discovery of new eIF5A hypusination inhibitors., PMID:39937781
The structural biology of deoxyhypusination complexes., PMID:39809274
Aspartate signalling drives lung metastasis via alternative translation., PMID:39743589
CircHIPK3 regulates cementoblast differentiation via the miR-10b-5p/DOHH/NF-κB axis., PMID:39304099
The Many Faces of Hypusinated eIF5A: Cell Context-Specific Effects of the Hypusine Circuit and Implications for Human Health., PMID:39125743
Exploring the Antiangiogenic and Anti-Inflammatory Potential of Homoisoflavonoids: Target Identification Using Biotin Probes., PMID:39062499
Analysis of network expression and immune infiltration of disulfidptosis-related genes in chronic obstructive pulmonary disease., PMID:38578019
Hypusine Signaling Promotes Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension., PMID:38261723
Construction of iron metabolism-related prognostic features of gastric cancer based on RNA sequencing and TCGA database., PMID:37957566
Integrative proteome and metabolome analyses reveal molecular basis underlying growth and nutrient composition in the Pacific oyster, Crassostrea gigas., PMID:37838097
Deoxyhypusine hydroxylase: A novel therapeutic target differentially expressed in short-term vs long-term survivors of glioblastoma., PMID:37141410
Ciclopirox Inhibition of eIF5A Hypusination Attenuates Fibroblast Activation and Cardiac Fibrosis., PMID:36826549
A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery., PMID:36725334
The pleiotropic roles of eIF5A in cellular life and its therapeutic potential in cancer., PMID:36511302
Identification of NRAS Downstream Genes with CRISPR Activation Screening., PMID:36358254
Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression., PMID:36057633
Biophysical and modeling-based approach for the identification of inhibitors against DOHH from Leishmania donovani., PMID:35809341
Bracovirus Sneaks Into Apoptotic Bodies Transmitting Immunosuppressive Signaling Driven by Integration-Mediated eIF5A Hypusination., PMID:35664011
Investigation of an Allosteric Deoxyhypusine Synthase Inhibitor in P. falciparum., PMID:35458660
[ORF19.2286 Gene: Isolation and Purification of Deoxyhypusine Hydroxylase from the Human Pathogenic Yeast Candida albicans]., PMID:35403624
Pentoxifylline-induced protein expression change in RAW 264.7 cells as determined by immunoprecipitation-based high performance liquid chromatography., PMID:35333871
The First Report of a Missense Variant in RFX2 Causing Non-Syndromic Tooth Agenesis in a Consanguineous Pakistani Family., PMID:35145545
Spermidine Regulates Mitochondrial Function by Enhancing eIF5A Hypusination and Contributes to Reactive Oxygen Species Production and Ganoderic Acid Biosynthesis in Ganoderma lucidum., PMID:35108082
Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification., PMID:35007708
Hypusinated EIF5A as a feasible drug target for Advanced Medicinal Therapies in the treatment of pathogenic parasites and therapy-resistant tumors., PMID:35000000
Neuron-specific ablation of eIF5A or deoxyhypusine synthase leads to impairments in growth, viability, neurodevelopment, and cognitive functions in mice., PMID:34688659
Post-translational formation of hypusine in eIF5A: implications in human neurodevelopment., PMID:34273022
Hypusination, a Metabolic Posttranslational Modification of eIF5A in Plants during Development and Environmental Stress Responses., PMID:34206171
Design, synthesis and biological evaluation of imidazopyridazine derivatives containing isoquinoline group as potent MNK1/2 inhibitors., PMID:33971490
Insights Into the Binding Mechanism of GC7 to Deoxyhypusine Synthase in Sulfolobus solfataricus: A Thermophilic Model for the Design of New Hypusination Inhibitors., PMID:33392152
Blockade of EIF5A hypusination limits colorectal cancer growth by inhibiting MYC elongation., PMID:33303756
LC-MS proteomic profiling of Caco-2 human intestinal cells exposed to the copper-chelating agent, triethylenetetramine: A preliminary study., PMID:32046857
Targeting B-cell malignancies with the beta-emitting anti-CD37 radioimmunoconjugate 177Lu-NNV003., PMID:31309259
Epstein-Barr virus (EBV) activates NKL homeobox gene HLX in DLBCL., PMID:31141539
Clinical significance of metabolism-related biomarkers in non-Hodgkin lymphoma - MCT1 as potential target in diffuse large B cell lymphoma., PMID:30790227
Recessive Rare Variants in Deoxyhypusine Synthase, an Enzyme Involved in the Synthesis of Hypusine, Are Associated with a Neurodevelopmental Disorder., PMID:30661771
Hypusine, a polyamine-derived amino acid critical for eukaryotic translation., PMID:30257869
Translation Factor eIF5A, Modification with Hypusine and Role in Regulation of Gene Expression. eIF5A as a Target for Pharmacological Interventions., PMID:30208826
The role of eukaryotic translation initiation factor 5A-1 (eIF5A-1) gene in HPV 16 E6 induces cell growth in human cervical squamous carcinoma cells., PMID:30170728
Targeting deoxyhypusine hydroxylase activity impairs cap-independent translation initiation driven by the 5'untranslated region of the HIV-1, HTLV-1, and MMTV mRNAs., PMID:27633452
Targeting eIF5A Hypusination Prevents Anoxic Cell Death through Mitochondrial Silencing and Improves Kidney Transplant Outcome., PMID:27612998
A suggested vital function for eIF-5A and dhs genes during murine malaria blood-stage infection., PMID:27516964
Targeting the polyamine-hypusine circuit for the prevention and treatment of cancer., PMID:27357307
Repositioning the Old Fungicide Ciclopirox for New Medical Uses., PMID:27238364
Drug-Based Lead Discovery: The Novel Ablative Antiretroviral Profile of Deferiprone in HIV-1-Infected Cells and in HIV-Infected Treatment-Naive Subjects of a Double-Blind, Placebo-Controlled, Randomized Exploratory Trial., PMID:27191165
Posttranslational hypusination of the eukaryotic translation initiation factor-5A regulates Fusarium graminearum virulence., PMID:27098988
Antitumour activity of the glycoengineered type II anti-CD20 antibody obinutuzumab (GA101) in combination with the MDM2-selective antagonist idasanutlin (RG7388)., PMID:26993060
The Beauty of a Visualized Peroxo-diiron(III) Intermediate., PMID:25955100
For research use only. Not for human or drug use.
