Catalog No.
YHK03201
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gln456-Ser643
Predicted molecular weight
23.69 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9UKV5
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Autocrine motility factor receptor, AMFR, E3 ubiquitin-protein ligase AMFR, RNF45, AMF receptor, gp78, RING finger protein 45
The autocrine motility factor receptor delays the pathological progression of Alzheimer's disease via regulating the ubiquitination-mediated degradation of APP., PMID:40301979
AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity., PMID:39505910
Impaired STING Activation Due to a Variant in the E3 Ubiquitin Ligase AMFR in a Patient with Severe VZV Infection and Hemophagocytic Lymphohistiocytosis., PMID:38277122
Gp78 deficiency in hepatocytes alleviates hepatic ischemia-reperfusion injury via suppressing ACSL4-mediated ferroptosis., PMID:38065978
AMFR dysfunction causes autosomal recessive spastic paraplegia in human that is amenable to statin treatment in a preclinical model., PMID:37119330
Staphylococcal virulence factor HlgB targets the endoplasmic-reticulum-resident E3 ubiquitin ligase AMFR to promote pneumonia., PMID:36593296
Expression of autocrine motility factor receptor (AMFR) in human breast and lung invasive micropapillary carcinomas., PMID:36576071
Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice., PMID:35938532
Autocrine motility factor receptor as a therapeutic target for asthma: comments on 'AMFR drives allergic asthma development by promoting alveolar macrophage-derived GM-CSF production'., PMID:35561732
AMFR drives allergic asthma development by promoting alveolar macrophage-derived GM-CSF production., PMID:35333296
iASPP suppresses Gp78-mediated TMCO1 degradation to maintain Ca2+ homeostasis and control tumor growth and drug resistance., PMID:35121659
Chemokine CCL1 as a therapeutic target for pulmonary fibrosis: comments on 'The chemokine CCL1 triggers an AMFR‒SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis'., PMID:34940827
RETREG1/FAM134B mediated autophagosomal degradation of AMFR/GP78 and OPA1 -a dual organellar turnover mechanism., PMID:32559118
Achieving pure spin effects by artifact suppression in methyl adiabatic relaxation experiments., PMID:32333192
Competitive oxidation and ubiquitylation on the evolutionarily conserved cysteine confer tissue-specific stabilization of Insig-2., PMID:31953408
POLE mutations improve the prognosis of endometrial cancer via regulating cellular metabolism through AMF/AMFR signal transduction., PMID:31864301
A technique for delineating the unfolding requirements for substrate entry into retrotranslocons during endoplasmic reticulum-associated degradation., PMID:31748412
Induction via Functional Protein Stabilization of Hepatic Cytochromes P450 upon gp78/Autocrine Motility Factor Receptor (AMFR) Ubiquitin E3-Ligase Genetic Ablation in Mice: Therapeutic and Toxicological Relevance., PMID:31492698
PINK1 Content in Mitochondria is Regulated by ER-Associated Degradation., PMID:31300519
GP78 Cooperates with Dual-Specificity Phosphatase 1 To Stimulate Epidermal Growth Factor Receptor-Mediated Extracellular Signal-Regulated Kinase Signaling., PMID:31061093
Gpnmb secreted from liver promotes lipogenesis in white adipose tissue and aggravates obesity and insulin resistance., PMID:32694855
Autocrine motility factor promotes endometrial cancer progression by targeting GPER-1., PMID:30836961
The ubiquitin specific protease USP34 protects the ubiquitin ligase gp78 from proteasomal degradation., PMID:30585151
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1., PMID:30543180
Calmodulin regulates MGRN1-GP78 interaction mediated ubiquitin proteasomal degradation system., PMID:30230921
Gp78 involvement in cellular proliferation: Can act as a promising modulator for cell cycle regulatory proteins?, PMID:29741771
Ubc7/Ube2g2 ortholog in Entamoeba histolytica: connection with the plasma membrane and phagocytosis., PMID:29594345
Ring finger protein 145 (RNF145) is a ubiquitin ligase for sterol-induced degradation of HMG-CoA reductase., PMID:29374057
A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes., PMID:29275994
Role of HSPA1L as a cellular prion protein stabilizer in tumor progression via HIF-1α/GP78 axis., PMID:28759037
Role of glycoprotein 78 and cidec in hepatic steatosis., PMID:28656280
Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation., PMID:28604676
Endoplasmic reticulum stress-induced degradation of DNAJB12 stimulates BOK accumulation and primes cancer cells for apoptosis., PMID:28536268
Conformational Dynamics and Allostery in E2:E3 Interactions Drive Ubiquitination: gp78 and Ube2g2., PMID:28434917
SVIP regulates Z variant alpha-1 antitrypsin retro-translocation by inhibiting ubiquitin ligase gp78., PMID:28301499
Correlation between the GP78 Gene Polymorphism and Coronary Atherosclerotic Heart Disease., PMID:28212872
Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions., PMID:27387505
Hepatic cytochromes P450: structural degrons and barcodes, posttranslational modifications and cellular adapters in the ERAD-endgame., PMID:27320797
AMFR gene silencing inhibits the differentiation of porcine preadipocytes., PMID:27173213
The linkage specificity determination of Ube2g2-gp78 mediated polyubiquitination., PMID:27067047
Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis., PMID:26743086
gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum., PMID:26424800
p38 MAP kinase-dependent phosphorylation of the Gp78 E3 ubiquitin ligase controls ER-mitochondria association and mitochondria motility., PMID:26337390
Canalicular membrane MRP2/ABCC2 internalization is determined by Ezrin Thr567 phosphorylation in human obstructive cholestasis., PMID:26212029
Autocrine motility factor promotes epithelial-mesenchymal transition in endometrial cancer via MAPK signaling pathway., PMID:26201353
Autocrine motility factor receptor promotes the proliferation of human acute monocytic leukemia THP-1 cells., PMID:26136223
Distinct mechanisms controlling rough and smooth endoplasmic reticulum contacts with mitochondria., PMID:26065430
Prognostic significance of autocrine motility factor receptor expression by colorectal cancer and lymph node metastases., PMID:25852009
Gp78, an E3 ubiquitin ligase acts as a gatekeeper suppressing nonalcoholic steatohepatitis (NASH) and liver cancer., PMID:25789613
Affinity proteomics discovers decreased levels of AMFR in plasma from Osteoporosis patients., PMID:25689831
For research use only. Not for human or drug use.
