Catalog No.
KHD27401
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human sVEGFR1 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human sVEGFR1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled specific antibody for Human sVEGFR1 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human sVEGFR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Human sVEGFR1 concentration in serum, plasma, cell lysates and cell culture supernates.
Range
78.13 - 5,000 pg/mL
Sensitivity
43.46 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
For research use only. Not for human or drug use.
