Overview
Catalog No.
RHN83802
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
EIF2A, Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9BY44
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R4A47
Data Image
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Flow cytometric analysis of HepG2 cells using EIF2A mouse mAb (green) and negative control (red).
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Immunofluorescence analysis of HepG2 cells using EIF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using EIF2A mouse mAb with DAB staining.
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Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.
References
Endoplasmic reticulum-stress and unfolded protein response-activation in immune-mediated necrotizing myopathy., PMID:35703068
Quantification of eIF2alpha phosphorylation during immunogenic cell death., PMID:31727256
The Effect of Culture Temperature on the Aggregation of Recombinant TNFR-Fc is Regulated by the PERK-eIF2a Pathway in CHO Cells., PMID:29848262
Sorafenib, a multikinase inhibitor, induces formation of stress granules in hepatocarcinoma cells., PMID:26556863
Expression of eukaryotic translation initiation factors 4E and 2alpha correlates with the progression of thyroid carcinoma., PMID:12186496
Developmental regulation of a plant encoded inhibitor of eukaryotic initiation factor 2 alpha phosphorylation., PMID:9301090
Datasheet
For research use only. Not for human or drug use.
