Anti-JCHAIN Antibody (R4A19)

Catalog No.

RHN54501

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

IHC: 1:500, WB: 1:200

Target

IGCJ, Immunoglobulin J chain, IGJ, JCHAIN, Joining chain of multimeric IgA and IgM

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01591

Applications

IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R4A19

Understanding IgM Structure and Biology to Engineer New Antibody Therapeutics., PMID:40237925

Modification of Fc-fusion protein structures to enhance efficacy of cancer vaccine in plant expression system., PMID:39724301

Asymmetric N-Glycosylation in the Tailpiece of Recombinant IgA1., PMID:39641195

How J-chain ensures the assembly of immunoglobulin IgM pentamers., PMID:39632981

Proteomics Reveals Age as Major Modifier of Inflammatory CSF Signatures in Multiple Sclerosis., PMID:39536291

Not All Arms of IgM Are Equal: Following Hinge-Directed Cleavage by Online Native SEC-Orbitrap-Based CDMS., PMID:38767111

Jchain-DTR Mice Allow for Diphtheria Toxin-Mediated Depletion of Antibody-Secreting Cells and Evaluation of Their Differentiation Kinetics., PMID:38766257

Possible transport routes of IgM to the gut of teleost fish., PMID:38657879

Mucosal immune responses and protective efficacy elicited by oral administration AMP-ZnONPs-adjuvanted inactivated H9N2 virus in chickens., PMID:38330890

Functional studies with IgM and IgA immunoglobulins: binding to pIgR, FcαμR, FcμR, and CDC activities., PMID:38232051

The immunoglobulin J chain is an evolutionarily co-opted chemokine., PMID:38215184

Temporal Tracking of Plasma Cells in vivo Using J-chain CreERT2 Reporter System., PMID:38106171

Each N-glycan on human IgA and J-chain uniquely affects oligomericity and stability., PMID:38070292

CD5L is a canonical component of circulatory IgM., PMID:38055740

Absolute Quantification of Pan-Cancer Plasma Proteomes Reveals Unique Signature in Multiple Myeloma., PMID:37835457

Single-Cell Network-Based Drug Repositioning for Discovery of Therapies against Anti-Tumour Necrosis Factor-Resistant Crohn's Disease., PMID:37762402

Mechanistic characterization of disulfide bond reduction of an ERAD substrate mediated by cooperation between ERdj5 and BiP., PMID:37739037

IGJ suppresses breast cancer growth and metastasis by inhibiting EMT via the NF‑κB signaling pathway., PMID:37539706

The human bone marrow plasma cell compartment in rheumatoid arthritis - Clonal relationships and anti-citrulline autoantibody producing cells., PMID:37001434

Broadly applicable TCR-based therapy for multiple myeloma targeting the immunoglobulin J chain., PMID:36850001

Capillary gel electrophoresis of very high molecular weight glycoproteins. Commercial and tailor-made gels for analysis of human monomeric and secretory immunoglobulin A., PMID:36528901

Identification of common and distinct origins of human serum and breastmilk IgA1 by mass spectrometry-based clonal profiling., PMID:36447030

Enhancement of intestinal mucosal immunity and immune response to the foot-and-mouth disease vaccine by oral administration of danggui buxue decoction., PMID:36425118

Cryomicroscopy reveals the structural basis for a flexible hinge motion in the immunoglobulin M pentamer., PMID:36274064

The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides., PMID:35675749

The teleost polymeric Ig receptor counterpart in ballan wrasse (Labrus bergylta) differs from pIgR in higher vertebrates., PMID:35605416

Yeast cell wall upregulated cell-mediated immune responses to Newcastle disease virus vaccine., PMID:35123352

3D Structures of IgA, IgM, and Components., PMID:34884580

The prospect of orally administered monoclonal secretory IgA (SIgA) antibodies to prevent enteric bacterial infections., PMID:34491878

Recombinant Human Secretory IgA Induces Salmonella Typhimurium Agglutination and Limits Bacterial Invasion into Gut-Associated Lymphoid Tissues., PMID:33728898

Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture Dissociation., PMID:33570406

Structure of the human secretory immunoglobulin M core., PMID:33513362

Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover., PMID:33136000

Structural insights into secretory immunoglobulin A and its interaction with a pneumococcal adhesin., PMID:32398862

Structural insights into immunoglobulin M., PMID:32029689

MZB1 promotes the secretion of J-chain-containing dimeric IgA and is critical for the suppression of gut inflammation., PMID:31127044

Glycan-independent binding and internalization of human IgM to FCMR, its cognate cellular receptor., PMID:28230186

Human Secretory IgM Antibodies Activate Human Complement and Offer Protection at Mucosal Surface., PMID:27864913

Effect of Bovine Lactoferrin Treatment Followed by Acute Stress on the IgA-Response in Small Intestine of BALB/c Mice., PMID:27611298

Novel Monoclonal Antibodies for Studies of Human and Rhesus Macaque Secretory Component and Human J-Chain., PMID:27386924

Site-Specific N-Glycosylation of Recombinant Pentameric and Hexameric Human IgM., PMID:27038031

Glutamine promotes intestinal SIgA secretion through intestinal microbiota and IL-13., PMID:27005687

Protective Effect of Moderate Exercise for BALB/c Mice with Salmonella Typhimurium Infection., PMID:26528943

Tracheal and bronchial polymeric immunoglobulin secretory immune system (PISIS) development in a porcine model., PMID:26188097

Morphological and immunohistochemical analyses of soluble proteins in mucous membranes of living mouse intestines by cryotechniques., PMID:25770106

Inhibition of B lymphocyte-induced maturation protein-1 reduces the production of autoantibody and alleviates symptoms of systemic lupus erythematosus., PMID:25347333

Phenotypes and distribution of mucosal memory B-cell populations in the SIV/SHIV rhesus macaque model., PMID:24814239

Development and use of IgM/J-chain fusion proteins for characterization of immunoglobulin superfamily ligand-receptor interactions., PMID:24510674

Effect of a tail piece cysteine deletion on biochemical and functional properties of an epidermal growth factor receptor-directed IgA2m(1) antibody., PMID:24492345

Noncoordinate expression of J-chain and Blimp-1 define nurse shark plasma cell populations during ontogeny., PMID:23897025

Document Download

Anti-JCHAIN Antibody (R4A19).pdf