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Anti-SYCP3 Antibody (R4A02)

Catalog #:   RHN28601 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC
Accession: Q8IZU3
Overview

Catalog No.

RHN28601

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000

Target

Synaptonemal complex protein 3, SCP3, SYCP3, SCP-3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q8IZU3

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R4A02

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using SYCP3 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HepG2 cells using SYCP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using SYCP3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human kidney tissues using SYCP3 mouse mAb with DAB staining.
References

Immunohistochemical Characterization of Spermatogenesis in the Ascidian Ciona robusta., PMID:39594612

A novel sorting method for the enrichment of early human spermatocytes from clinical biopsies., PMID:38369016

Three-color dSTORM Imaging and Analysis of Recombination Foci in Mouse Spread Meiotic Nuclei., PMID:37497444

Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set., PMID:37077350

A molecular cell biology toolkit for the study of meiosis in the silkworm Bombyx mori., PMID:36911915

Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes., PMID:35052491

Meiotic self-pairing of the Psalidodon (Characiformes, Characidae) iso-B chromosome: A successful perpetuation mechanism., PMID:34617950

Interaction between SCP3 and JAB1 Confers Cancer Therapeutic Resistance and Stem-like Properties through EGF Expression., PMID:34445562

Kinase CDK2 in Mammalian Meiotic Prophase I: Screening for Hetero- and Homomorphic Sex Chromosomes., PMID:33671248

Isolation, identification and differentiation of human spermatogonial cells on three-dimensional decellularized sheep testis., PMID:32992121

Heterochiasmy and Sexual Dimorphism: The Case of the Barn Swallow (Hirundo rustica, Hirundinidae, Aves)., PMID:32987748

Molecular analysis of the effects of steroid hormones on mouse meiotic prophase I progression., PMID:31791345

Differentiation of spermatogonial stem cells by soft agar three-dimensional culture system., PMID:31131631

Photobiomodulation and gametogenic potential of human Wharton's jelly-derived mesenchymal cells., PMID:31029424

Disassembly of the synaptonemal complex in chicken oocytes analyzed by super-resolution microscopy., PMID:30793238

In vitro differentiation of human oocyte-like cells from oogonial stem cells: single-cell isolation and molecular characterization., PMID:29304224

Meiotic Recombination in the Giraffe (G. reticulata)., PMID:28723680

Regulatory complexity revealed by integrated cytological and RNA-seq analyses of meiotic substages in mouse spermatocytes., PMID:27519264

The cryptic Y-autosome translocation in the small Indian mongoose, Herpestes auropunctatus, revealed by molecular cytogenetic approaches., PMID:26743516

Promoting effect of licorice extract on spermatogonial proliferation and spermatocytes differentiation of neonatal mice in vitro., PMID:26676954

Variation of Meiotic Recombination Rates and MLH1 Foci Distribution in Spermatocytes of Cattle, Sheep and Goats., PMID:26406935

Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways., PMID:26088866

Identification and Characterization of Xlr5c as a Novel Nuclear Localization Protein in Mouse Germ Cells., PMID:26075718

A rare Robertsonian translocation rob(14;22) carrier with azoospermia, meiotic defects, and testicular sperm aneuploidy., PMID:26043179

Surface-spreading technique of meiotic cells and immunodetection of synaptonemal complex proteins in teleostean fishes., PMID:25642289

The zinc-finger protein basonuclin 2 is required for proper mitotic arrest, prevention of premature meiotic initiation and meiotic progression in mouse male germ cells., PMID:25344072

Use of in ovo chorioallantoic membrane engraftment to culture testes from neonatal mice., PMID:25296013

Dissociation of the X chromosome from the synaptonemal complex in the XY body of the rodent Galea musteloides., PMID:24043547

Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish., PMID:21402175

[How do chromosomes attach to synaptonemal complexes?]., PMID:21254557

The multi-copy mouse gene Sycp3-like Y-linked (Sly) encodes an abundant spermatid protein that interacts with a histone acetyltransferase and an acrosomal protein., PMID:19176879

Synaptonemal complex protein SYCP3 of the rat: evolutionarily conserved domains and the assembly of higher order structures., PMID:18391527

Mammalian neo-oogenesis and expression of meiosis-specific protein SCP3 in adult human and monkey ovaries., PMID:18256545

Sperm aneuploidy and meiotic sex chromosome configurations in an infertile XYY male., PMID:18037669

Differential distribution and association of repeat DNA sequences in the lateral element of the synaptonemal complex in rat spermatocytes., PMID:17968579

Expression analysis of the mouse multi-copy X-linked gene Xlr-related, meiosis-regulated (Xmr), reveals that Xmr encodes a spermatid-expressed cytoplasmic protein, SLX/XMR., PMID:17475928

Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes., PMID:16764855

Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes., PMID:16235117

Synapsis and meiotic recombination analyses: MLH1 focus in the XY pair as an indicator., PMID:15890739

Female-specific features of recombinational double-stranded DNA repair in relation to synapsis and telomere dynamics in human oocytes., PMID:15235794

Kinetics of meiosis in azoospermic males: a joint histological and cytological approach., PMID:15218256

Cohesin component dynamics during meiotic prophase I in mammalian oocytes., PMID:15125634

Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis., PMID:12759374

Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1beta and SMC3., PMID:12615909

Mammalian STAG3 is a cohesin specific to sister chromatid arms in meiosis I., PMID:11483963

Meiotic telomere distribution and Sertoli cell nuclear architecture are altered in Atm- and Atm-p53-deficient mice., PMID:11003672

Localisation of RAD50 and MRE11 in spermatocyte nuclei of mouse and rat., PMID:10855503

Fate of meiotic lamin C2 in rat spermatocytes cultured in the presence of okadaic acid., PMID:10855502

The meiotic specific synaptonemal complex protein SCP3 is expressed by female and male primordial germ cells of the mouse embryo., PMID:10794082

Association of mammalian SMC1 and SMC3 proteins with meiotic chromosomes and synaptonemal complexes., PMID:10652260

Datasheet

Document Download

Anti-SYCP3 Antibody (R4A02).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SYCP3 Antibody (R4A02) [RHN28601]
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