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Anti-HLA-B Antibody (R3Z69)

Catalog #:   RHM00203 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: P01889
Overview

Catalog No.

RHM00203

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

HLA-B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, HLAB

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01889

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Z69

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using HLA-B mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using HLA-B mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using HLA-B mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using HLA-B mouse mAb against Ramos (1) and A431 (2) cell lysate.
References

Donor-specific antibodies against HLA-C, HLA-DP and HLA-DQ and their implications in kidney transplantation., PMID:40535491

Comparison of the Mutational Profile between BCL2- and BCL6-Rearrangement Positive Follicular Lymphoma., PMID:40482882

Engaging T cells for cleanup., PMID:40416957

Case Report: Ankylosing Spondylitis Complicated With Systemic Lupus Erythematosus., PMID:40349144

Enhancing the Anticancer Activity of a Carcinoma-Directed Peptide-HLA-I Fusion Protein by Armoring with Mutein IFNα., PMID:40243928

The influence of genetic factors on the clinical manifestations and response to systemic treatment of plaque psoriasis., PMID:40095058

HLA Association With AQP4-IgG-Positive Neuromyelitis Optica Spectrum Disorder in the Korean Population., PMID:40020215

Genetic associations with measles PRNT and IgG antibody response to MMR vaccination in 6- and 15-month-old children., PMID:39914254

In Situ Detection of Individual Classic MHC-I Gene Products in Cancer., PMID:39804685

Depletion of alloreactive B cells by drug-resistant chimeric alloantigen receptor T cells to prevent transplant rejection., PMID:39799394

HLA and Non-HLA Factors for Donor Selection in Hematopoietic Stem Cell Transplantation with Post-Transplant Cyclophosphamide GvHD Prophylaxis., PMID:39768158

HLA Class I and II Alleles in Anti-Acetylcholine Receptor Antibodies Positive and Double-Seronegative Myasthenia Gravis Patients of Romanian Descent., PMID:39728756

Impact of repeat human leukocyte antigen mismatches on kidney graft survival: A contemporary Collaborative Transplant Study analysis., PMID:39716692

Cytomegalovirus-Specific T-Cell-Receptor-like Antibodies Target In Vivo-Infected Human Leukocytes Inducing Natural Killer Cell-Mediated Antibody-Dependent Cellular Cytotoxicity., PMID:39684614

Targeting peptide antigens using a multiallelic MHC I-binding system., PMID:39672954

HLA-EpiCheck: novel approach for HLA B-cell epitope prediction using 3D-surface patch descriptors derived from molecular dynamic simulations., PMID:39659590

HLA-B*15 Is Associated With SARS-CoV-2 Infection in a Brazilian Population., PMID:39530176

A new strategy for systematically classifying HLA alleles into serological specificities: Update and refinement., PMID:39435845

[The distribution of biomarkers for Behcet syndrome and their clinical relevance in real-world studies]., PMID:39290014

Converting an HLA-B27 flow assay from the BD FACSCanto to the BD FACSLyric., PMID:39287115

Individuals carrying the HLA-B*15 allele exhibit favorable responses to COVID-19 vaccines but are more susceptible to Omicron BA.5.2 and XBB.1.16 infection., PMID:39257586

Isatuximab for Delayed Red Cell Engraftment after Allogeneic Hematopoietic Cell Transplantation., PMID:39246802

Diagnosis of Graves' Disease during Treatment for Subacute Thyroiditis., PMID:39231676

Sustained HIV remission after allogeneic hematopoietic stem cell transplantation with wild-type CCR5 donor cells., PMID:39222660

Profile of immunological biomarkers in Behcet's syndrome: a large-scale single-center real-world study., PMID:39196452

Next-generation sequencing reveals additional HLA class I and class II alleles associated with type 1 diabetes and age at onset., PMID:39185409

Case report: Dupilumab: a promising treatment option for adult linear IgA bullous dermatosis with severe pruritus., PMID:39161758

The Structural Basis for Recognition of Human Leukocyte Antigen Class I Molecules by the Pan-HLA Antibody W6/32., PMID:39093013

Glycolysis Changes in Alloreactive Memory B Cells in Highly Sensitized Kidney Transplant Recipients Undergoing Desensitization Therapy., PMID:39081904

HLA-B*35:01-mediated activation of emodin-specific T cells contributes to Polygonum multiflorum thunb. -induced liver injury in mice., PMID:38969149

Homozygosity in any HLA locus is a risk factor for specific antibody production: the taboo concept 2.0., PMID:38840925

SOHO State of the Art Updates and Next Questions | Current Status and Future Directions of Donor Selection., PMID:38825445

Association of HLA B- and T-cell molecular mismatches with HLA antibodies, rejection, and graft survival in pediatric kidney transplantation., PMID:38808702

Anti-integrin αvβ6 antibody in Takayasu arteritis patients with or without ulcerative colitis., PMID:38784377

Dual role of the peptide-loading complex as proofreader and limiter of MHC-I presentation., PMID:38771881

Effect of HLA Genotype on Anti-PD-1 Antibody Treatment for Advanced Renal Cell Carcinoma in the SNiP-RCC Study., PMID:38758119

Analysis of immunogenetics interlaboratory comparisons' success rates. External quality assurance system of the Spanish Society for Immunology GECLID-SEI., PMID:38746054

Acute mixed-lineage leukemia treated with desensitization therapy prior to HLA-haploidentical transplantation with high donor-specific antibodies., PMID:38676859

Multi-omics study reveals different pathogenesis of the generation of skin lesions in SLE and IDLE patients., PMID:38643729

Establishment and clinical application of the HLA genotype database of platelet-apheresis donors in Suzhou., PMID:38638976

Pathologic Features of Anti-Ku Myositis., PMID:38547417

Human Leukocyte Antigen Markers for Distinguishing Pustular Psoriasis and Adult-Onset Immunodeficiency with Pustular Reaction., PMID:38540337

HLA allele frequency of HLA-A, -B, -C, -DRB1 and -DQB1 in Indian recurrent implantation failure and recurrent pregnancy loss couples - A retrospective study., PMID:38518419

Platelet transfusions in haploidentical haematopoietic stem cell allograft candidates: Protecting HLA-A and HLA-B antigens through eplet analysis., PMID:38516935

Mutational, immune microenvironment, and clinicopathological profiles of diffuse large B-cell lymphoma and follicular lymphoma with BCL6 rearrangement., PMID:38462571

[Analysis of transfusion effect of different platelet matching schemes in patients with platelet transfusion refractoriness]., PMID:38462363

Differentially Expressed Genes Involved in Primary Resistance to Immunotherapy in Patients with Advanced-Stage Pulmonary Cancer., PMID:38396726

Diversity in the HLA-I Recognition of HLA-F Monoclonal Antibodies: HLA-F or HLA-Ib Monospecific, HLA-E or HLA-G Bispecific Antibodies with or without HLA-Ia Reactivity., PMID:38390869

Anti-HLA-B7/HLA-B44 strong cross immunoreactivity observed in flow cytometry HLA-B27 immunotyping., PMID:38379087

Antibody verification of HLA class I and class II eplets by human monoclonal HLA antibodies., PMID:38239050

Datasheet

Document Download

Anti-HLA-B Antibody (R3Z69).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-HLA-B Antibody (R3Z69) [RHM00203]
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