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Anti-DDX20 Antibody (R3Z28)

Catalog #:   RHK68301 Specific References (8) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: Q9UHI6
Overview

Catalog No.

RHK68301

Species reactivity

Human, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

DP103, Gemin-3, DDX20, Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein DP 103, DEAD box protein 20, GEMIN3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9UHI6

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Z28

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.
References

Epstein-Barr virus nuclear antigen 3C stabilizes Gemin3 to block p53-mediated apoptosis., PMID:22174681

Abnormal interaction of motor neuropathy-associated mutant HspB8 (Hsp22) forms with the RNA helicase Ddx20 (gemin3)., PMID:20157854

Gemin proteins are required for efficient assembly of Sm-class ribonucleoproteins., PMID:16301532

Functional cooperation of Epstein-Barr virus nuclear antigen 2 and the survival motor neuron protein in transactivation of the viral LMP1 promoter., PMID:11689659

Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins., PMID:10942426

Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli., PMID:10725331

Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems., PMID:10601333

Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C., PMID:10383418

Datasheet

Document Download

Anti-DDX20 Antibody (R3Z28).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-DDX20 Antibody (R3Z28) [RHK68301]
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