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Anti-Phospho-Tyrosine Antibody (R3Z20)

Catalog #:   RGK60105 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC, WB
Overview

Catalog No.

RGK60105

Species reactivity

General

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:50-1:100, WB: 1:500-1:1000

Target

Phospho-Tyrosine, p-Tyrosine, Tyrosine (p), Tyr (p), Y (p)

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Z20

Data Image
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human Breast Caricnoma using Phospho-tyrosine antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of Phospho-Tyrosine (6G6)in Jurkat, 293T, rat Brain, mouse Brain lysates using Phospho-tyrosine antibody.
References

DeepPhoPred: Accurate Deep Learning Model to Predict Microbial Phosphorylation., PMID:39239684

The ion channel TRPV5 regulates B-cell signaling and activation., PMID:38694510

BTK kinase activity is dispensable for the survival of diffuse large B-cell lymphoma., PMID:36183831

NLRP3 Phospho-residue Mapping by Phospho Dot Blots., PMID:35212958

Ganglioside GD2 Enhances the Malignant Phenotypes of Melanoma Cells by Cooperating with Integrins., PMID:35008849

Performance of phospho-L-tyrosine immobilized onto alginate/polyacrylamide-based cryogels: Effect of ligand coupling on human IgG adsorption and Fab fragments separation., PMID:33486219

PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation., PMID:32130916

The combination of bevacizumab/Avastin and erlotinib/Tarceva is relevant for the treatment of metastatic renal cell carcinoma: the role of a synonymous mutation of the EGFR receptor., PMID:31938054

Betacellulin drives therapy resistance in glioblastoma., PMID:31678994

Polyacrylamide-alginate (PAAm-Alg) and phospho-L-tyrosine-linked PAAm-Alg monolithic cryogels: Purification of IgG from human serum., PMID:31536838

Phospho-l-tyrosine-agarose chromatography: Adsorption of human IgG and its proteolytic fragments., PMID:30246450

The role of the ShcD and RET interaction in neuroblastoma survival and migration., PMID:29556564

Phosphorylated-tyrosine based pseudobioaffinity adsorbent for the purification of immunoglobulin G., PMID:28343107

Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors., PMID:26445848

Phosphopeptide immuno-affinity enrichment to enhance detection of tyrosine phosphorylation in plants., PMID:25930699

Evaluation of different phospho-tyrosine antibodies for label-free phosphoproteomics., PMID:25890253

Preanalytical variables and phosphoepitope expression in FFPE tissue: quantitative epitope assessment after variable cold ischemic time., PMID:25418580

Phagocytic cells internalize ZnO particles by FcγII/III-receptor pathway., PMID:25082708

PECAM-1 phosphorylation and tissue factor expression in HUVECs exposed to uniform and disturbed pulsatile flow and chemical stimuli., PMID:24342062

A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization., PMID:24003221

Fc gamma receptor CD64 modulates the inhibitory activity of infliximab., PMID:22937039

TCR-induced T cell activation leads to simultaneous phosphorylation at Y505 and Y394 of p56(lck) residues., PMID:22674786

Impaired degradation followed by enhanced recycling of epidermal growth factor receptor caused by hypo-phosphorylation of tyrosine 1045 in RBE cells., PMID:22591401

Targeted quantitative phosphoproteomics approach for the detection of phospho-tyrosine signaling in plants., PMID:22074104

SAP-mediated inhibition of diacylglycerol kinase α regulates TCR-induced diacylglycerol signaling., PMID:22048771

PKCβ-dependent phosphorylation of the glycine transporter 1., PMID:21864610

TLRs innate immunereceptors and Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) CIDR1α-driven human polyclonal B-cell activation., PMID:21620790

Arsenic stress activates MAP kinase in rice roots and leaves., PMID:21081102

[New functional proteins identified by proteomic analysis in the epidermal growth factor receptor-mediated signaling pathway and application for practical use]., PMID:20371988

High-throughput kinase assay based on surface plasmon resonance., PMID:20217618

Estrogen receptor alpha phosphorylated at tyrosine 537 is associated with poor clinical outcome in breast cancer patients treated with tamoxifen., PMID:21761367

Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling., PMID:20005895

Amyloid precursor protein mediates a tyrosine kinase-dependent activation response in endothelial cells., PMID:19923279

Molecular imaging of gefitinib activity in an epidermal growth factor receptor (EGFR)-bearing xenograft model., PMID:19823028

A HIF-1alpha-dependent autocrine feedback loop promotes survival of serum-deprived prostate cancer cells., PMID:19016246

ERK1/2 mediate wounding- and G-protein-coupled receptor ligands-induced EGFR activation via regulating ADAM17 and HB-EGF shedding., PMID:18658095

Insulin growth factor 1 receptor/PI3K/AKT survival pathway in outer segment membranes of rod photoreceptors., PMID:18566464

[Global gene expression analysis and novel signalling pathways in Hodgkin lymphoma]., PMID:17867590

The radioprotector O-phospho-tyrosine stimulates DNA-repair via epidermal growth factor receptor- and DNA-dependent kinase phosphorylation., PMID:17714814

Bisperoxovanadium, a phospho-tyrosine phosphatase inhibitor, reprograms myogenic cells to acquire a pluripotent, circulating phenotype., PMID:17601985

Homogeneous time-resolved fluorescence assays for the detection of activity and inhibition of phosphatase enzymes employing phosphorescently labeled peptide substrates., PMID:17386566

Proteomic analysis of tyrosine phosphorylations in vascular endothelial growth factor- and reactive oxygen species-mediated signaling pathway., PMID:17269716

Protein tyrosine phosphorylation in the transition to light state 2 of chloroplast thylakoids., PMID:16228330

Okadaic acid induces tyrosine phosphorylation of IkappaBalpha that mediated by PKR pathway in human osteoblastic MG63 cells., PMID:16132703

Cross-linking of 4-1BB activates TCR-signaling pathways in CD8+ T lymphocytes., PMID:15699116

Onychin inhibits proliferation of vascular smooth muscle cells by regulating cell cycle., PMID:15663900

Vascular endothelial cadherin and beta-catenin in human fetoplacental vessels of pregnancies complicated by Type 1 diabetes: associations with angiogenesis and perturbed barrier function., PMID:15298347

Keap1 in adhesion complexes., PMID:14506708

Post-mortem interval effects on the phosphorylation of signaling proteins., PMID:12637955

The Polycomb-group protein ENX-2 interacts with ZAP-70., PMID:12600746

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-Phospho-Tyrosine Antibody (R3Z20) [RGK60105]
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