Overview
Catalog No.
RHK46902
Species reactivity
Human
Host species
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
UFD1L, Ubiquitin recognition factor in ER-associated degradation protein 1, UB fusion protein 1, UFD1, Ubiquitin fusion degradation protein 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q92890
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3Z02
Data Image
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Flow cytometric analysis of Hela cells using UFD1L mouse mAb (green) and negative control (red).
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Immunofluorescence analysis of Hela cells using UFD1L mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Immunofluorescence analysis of Hela cells using UFD1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using UFD1L mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using UFD1L mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using UFD1L mouse mAb with DAB staining.
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Western blot analysis using UFD1L mouse mAb against K562 (1), Hela (2), A431 (3), PC-2 (4), and A549 (5) cell lysate.
References
TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing., PMID:37816088
Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer., PMID:32521270
Serum biomarkers in patients suspected of transient ischaemic attack in primary care: a diagnostic accuracy study., PMID:31628130
Cdc48/p97-Ufd1-Npl4 antagonizes Aurora B during chromosome segregation in HeLa cells., PMID:21486945
Analysis of Npl4 deletion mutants in mammalian cells unravels new Ufd1-interacting motifs and suggests a regulatory role of Npl4 in ERAD., PMID:18586029
Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies., PMID:12910480
Structure and expression of the human ubiquitin fusion-degradation gene (UFD1L)., PMID:9540831
Datasheet
For research use only. Not for human or drug use.
