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Anti-DDX1 Antibody (R3Y91)

Catalog #:   RHK42201 Specific References (5) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q92499
Overview

Catalog No.

RHK42201

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

DEAD box protein retinoblastoma, ATP-dependent RNA helicase DDX1, DEAD box protein 1, DBP-RB, DDX1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q92499

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Y91

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using DDX1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DDX1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using DDX1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using DDX1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), and Jurkat (5) cell lysate.
References

Phosphorylation impacts GLE1 nuclear localization and association with DDX1., PMID:37801910

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination., PMID:29731414

DEAD box 1: a novel and independent prognostic marker for early recurrence in breast cancer., PMID:20499159

Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA., PMID:11598190

Overexpression of a DEAD box protein (DDX1) in neuroblastoma and retinoblastoma cell lines., PMID:9694872

Datasheet

Document Download

Anti-DDX1 Antibody (R3Y91).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Distributor list

For research use only. Not for human or drug use.

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Anti-DDX1 Antibody (R3Y91) [RHK42201]
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