Catalog No.
RGK25803
Species reactivity
Aequorea victoria (Water jellyfish) (Mesonema victoria)
Host species
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Tested applications
FCM: 1:200-1:400, IF: 1:100-1:200, IHC: 1:200-1:1000, WB: 1:500-1:1000
Target
GFP Tag, GFP-Tag, Green fluorescent protein
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P42212
Applications
FCM, IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3Y69
Unraveling Botulinum Neurotoxin A Light-Chain-Induced Signaling Pathways: A Phosphoproteomic Analysis in a Controlled Cellular Model., PMID:40507977
Combining CD3/GD2 bispecific T cell engager with human Vγ9Vδ2 T cells facilitates neuroblastoma cell targeting and killing in vitro., PMID:40489497
A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins., PMID:40485297
Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry., PMID:40483334
Live-cell imaging of a plant virus replicase during infection using a genetically encoded, antibody-based probe., PMID:40479561
Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein., PMID:39991067
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots., PMID:39185782
Super-Resolved Protein Imaging Using Bifunctional Light-Up Aptamers., PMID:39115976
A Single-step Generation of AlissAID-based Conditional Knockdown Strains Using Nanobody that Targets GFP or mCherry in Budding Yeast., PMID:38948262
Administration sequence- and formation-dependent vaccination using acid-degradable polymeric nanoparticles with high antigen encapsulation capability., PMID:38872501
Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis., PMID:38782081
Synthetic protein protease sensor platform., PMID:38646011
Thermodynamic and molecular dynamic insights into how fusion influences peptide-tag recognition of an antibody., PMID:38622354
Halo-RPD: searching for RNA-binding protein targets in plants., PMID:38465250
Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection., PMID:38314809
Multiplex Assays for Analysis of Antibody Responses to South Asian Plasmodium falciparum and Plasmodium vivax Malaria Infections., PMID:38276660
Split-GFP complementation at the bacterial cell surface for antibody-free labeling and quantification of heterologous protein display., PMID:38176324
Preclinical Evaluation of 99mTc-MAG3-5-Fab Targeting TREM2 in Lung Cancer Mouse Models: A Comparison with 99mTc-MAG3-5-F(ab')2., PMID:38109713
Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow Cytometry., PMID:37558955
Preparation and Utilization of a Versatile GFP-Protein Trap-Like System for Protein Complex Immunoprecipitation in Plants., PMID:37450136
Plant-Produced Mouse-Specific Zona Pellucida 3 Peptide Induces Immune Responses in Mice., PMID:36679998
Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development., PMID:36609424
Tissue-Specific, Genome-wide Mapping of R-loops in Drosophila Using MapR., PMID:36248608
Determining Protein-Protein Interaction with GFP-Trap Beads., PMID:36107351
High-efficiency recombinant protein purification using mCherry and YFP nanobody affinity matrices., PMID:36040252
The transmembrane domain of the amyloid precursor protein is required for antiamyloidogenic processing by α-secretase ADAM10., PMID:35398353
V5 and GFP Tagging of Viral Gene pp38 of Marek's Disease Vaccine Strain CVI988 Using CRISPR/Cas9 Editing., PMID:35216029
Immunofluorescence Detection of Plasma Membranous PTEN in Cultured Cells., PMID:35199573
Fixation and Immunostaining of Endogenous Proteins or Post-translational Modificationsin Caenorhabditis elegans., PMID:34722819
greenCUT&RUN: Efficient Genomic Profiling of GFP-Tagged Transcription Factors and Chromatin Regulators., PMID:34644460
Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo., PMID:34583743
Coiled coil exposure and histidine tags drive function of an intracellular protein drug carrier., PMID:34563592
Expression and Characterization of Two DNA Constructs Derived from HIV-1-vif in Escherichia coli and Mammalian Cells., PMID:34484642
Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool., PMID:34476263
GFP-Tagged Protein Detection by Electron Microscopy Using a GBP-APEX Tool in Drosophila., PMID:34476234
Transient Expression in Cytoplasm and Apoplast of Rotavirus VP6 Protein Fused to Anti-DEC205 Antibody in Nicotiana benthamiana and Nicotiana sylvestris., PMID:34146324
The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitro., PMID:34126963
Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins., PMID:33742097
Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes., PMID:33659473
A transgenic DND1GFP fusion allele reports in vivo expression and RNA-binding targets in undifferentiated mouse germ cells†., PMID:33394034
An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification., PMID:33303987
Direct Antibody Isolation on Cells Using Affinity-Tag-Guided Proximity Selection., PMID:33118810
Inducible Degradation of Target Proteins through a Tractable Affinity-Directed Protein Missile System., PMID:32668203
Generation and characterization of two strains of transgene mice expressing chimeric MiniSOG-MusPrP., PMID:32416277
Positive Selection of Specific Antibodies Produced against Fusion Proteins., PMID:32397084
Blast resistance gene Pi54 over-expressed in rice to understand its cellular and sub-cellular localization and response to different pathogens., PMID:32251298
Single-cell Analysis of β2-Adrenergic Receptor Dynamics by Quantitative Fluorescence Microscopy., PMID:31840614
Hypothalamic C2-domain protein involved in MC4R trafficking and control of energy balance., PMID:31666192
Unintended perturbation of protein function using GFP nanobodies in human cells., PMID:31601614
TLR5 is a new reporter for triple-negative breast cancer indicated by radioimmunoimaging and fluorescent staining., PMID:31576678
For research use only. Not for human or drug use.
