Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Recombinant Antibody

Anti-His Tag Antibody (R3Y39)

Catalog #:   RGK07905 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, IP, WB
Overview

Catalog No.

RGK07905

Species reactivity

General

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:100-1:1000, IHC: 1:100-1:1000, IP: 1:20, WB: 1:500-1:1000

Target

6 × His Tag (HHHHHH), 6HIS, 6*HIS

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

ELISA, FCM, IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Y39

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with His Tag antibody (RGK07905) at 1:1000.

    Lane 1: Recombinant protein ( 25 ng )
    Lane 2: Recombinant protein ( 10 ng )
    Lane 3: Recombinant protein ( 2.5 ng )

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 50 kDa
    Observed MW: 50 kDa
References

An Electrochemiluminescence Biosensor for the Screening of Environmental Estrogens Using a Peptide Probe Targeting the Activated Estrogen Receptor., PMID:40533042

In vitro anti-tumor activities of a novel recombinant immunotoxin targeting differentially overexpressed Leucine-rich repeat-containing G-protein-coupled receptor 5 in cervical cancer., PMID:40376858

The Function of TER94 of Spodoptera frugiperda 9 Cells When Infected With Invertebrate Iridescent Virus-6., PMID:40357853

Scarless excision of an insertion sequence in the OmpK36 promoter restores meropenem susceptibility in a non-carbapenemase-producing Klebsiella pneumoniae., PMID:40340575

Enhancing theranostic potential of anti-mesothelin sdAb through site-specific labeling at a unique conserved lysine by molecular engineering., PMID:40293556

Novel Soluble apxIVA-Truncated Protein and Its Application to Rapid Detection and Distinction of Actinobacillus pleuropneumoniae Wild-Strain-Infected Samples from Those Vaccinated with apxIV-Partially Deleted Vaccine., PMID:40266988

Recombinant receptor-binding motif of spike COVID-19 vaccine candidate induces SARS-CoV-2 neutralizing antibody response., PMID:40256231

Rational design and pairing of single-domain antibodies for developing rapid detection methods for botulinum neurotoxins A and B., PMID:40253065

Cloning and Recombinant Expression of the Caspase-Activated DNase Orthologous Gene of Giardia lamblia., PMID:40224544

Adding a Twist to Lateral Flow Immunoassays: A Direct Replacement of Antibodies with Helical Affibodies, from Selection to Application., PMID:40135773

50-Fold Adjuvant and 20-Fold Antigen Vaccine Dose Sparing from Nanoliposome Display of a Stabilized Malarial Protein Antigen., PMID:40099532

Seroprevalence of Getah virus in pigs in Southeast China determined with a recombinant Cap protein-based indirect ELISA., PMID:40034493

Performance of protein N linear epitopes in serodiagnosis of COVID-19 infection., PMID:39963569

Leishmania donovani homoserine dehydrogenase: Biochemical and structural characterization of a novel parasite specific enzyme of aspartate pathway., PMID:39961538

Development of the antigenic recombinant tropomyosin of Echinococcus granulosus in a bacterial system as a vaccinal candidate against canine echinococcosis., PMID:39936765

A high sensitivity assay of UBE3A ubiquitin ligase activity., PMID:39933617

Development of an enzymatic aptasensor for monitoring recombinant His-tagged proteins in microbial biotechnology., PMID:39929015

Expressing red fluorescent protein on the surface of Escherichia coli using C-terminal domain of autotransporters., PMID:39744513

Intranasal Immunization with Nasal Immuno-Inducible Sequence-Fused Antigens Elicits Antigen-Specific Antibody Production., PMID:39684539

Immuno-PET/CT Imaging of Trop2 with [18F]AlF-RESCA-T4 Differentiates Lung Cancer from Inflammation., PMID:39542697

Gold Nanoparticles-Based Colorimetric Immunoassay of Carcinoembryonic Antigen with Metal-Organic Framework to Load Quinones for Catalytic Oxidation of Cysteine., PMID:39460180

Development and Characterization of A Novel SpyTagged Modular Nanobody as A Detection Platform for CD22-Positive Cells., PMID:39380479

Generation of dengue 3 envelope domain III using tobacco mosaic virus-based vector system and its immunological response mouse model by generating anti-dengue virus antibodies., PMID:39380277

Effects of combined cytotoxic T-lymphocyte antigen 4 and programed death 1 ligand-receptor blockade on interferon-gamma production in bovine leukemia virus-infected cattle., PMID:39328455

Laminin 511 E8 fragment promotes to form basement membrane-like structure in human skin equivalents., PMID:39286641

Development and Characterization of an Anti-PD-L1 Immunotoxin for Targeted Cancer Therapy., PMID:39238385

99mTc(I)-Labeled His-Tagged Proteins: Impact in the Development of Novel Imaging Probes and in Drug Discovery., PMID:39158861

Cloning and expression profiling of voltage-gated sodium channel gene (VGSC) from Spodoptera frugiperda., PMID:39151677

Interfacial modification of recombinant protein for immunoglobulin G adsorption with spindle-shaped MOF as nano molecular containers., PMID:39121618

Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics., PMID:39100766

Efficient generation of recombinant anti-HER2 scFv with high yield and purity using a simple method., PMID:39014926

Displaying alphavirus physicochemical consensus antigens on immunogenic liposomes enhances antibody elicitation in mice., PMID:38968676

Molecular dynamics simulation and purification of chimeric L1/L2 protein from human papillomavirus type 52 expressed in Escherichia coli BL21 (DE3)., PMID:38965835

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina., PMID:38924064

The PCV3 Cap Virus-like Particle Vaccine with the Chimeric PCV2-Neutralizing Epitope Gene Is Effective in Mice., PMID:38922011

Highly selective chemical modification of poly-His tagged peptides and proteins., PMID:38886029

Administration sequence- and formation-dependent vaccination using acid-degradable polymeric nanoparticles with high antigen encapsulation capability., PMID:38872501

Isolation and Characterization of the Vascular Endothelial Growth Factor Receptor Targeting ScFv Antibody Fragments Derived from Phage Display Technology., PMID:38799304

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen., PMID:38791214

Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis., PMID:38782081

Development of a mutant aerosolized ACE2 that neutralizes SARS-CoV-2 in vivo., PMID:38771062

PSMA-reactive NB7 single domain antibody fragment: A potential scaffold for developing prostate cancer theranostics., PMID:38703588

Synthetic protein protease sensor platform., PMID:38646011

Determination, expression and characterization of an UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I (GnT-I) from the Pacific oyster, Crassostrea gigas., PMID:38557922

Preparation, Characterization, and Radiolabeling of Anti-HER2 scFv With Technetium Tricarbonyl and Stability Studies., PMID:38485465

ImmunoPET/CT imaging of clear cell renal cell carcinoma with [18F]RCCB6: a first-in-human study., PMID:38480552

Efficient Escorting Strategy for Aggregation-Prone Notch EGF Repeats with Sparcl1., PMID:38474544

Designing, cloning and simulation studies of cancer/testis antigens based multi-epitope vaccine candidates against cutaneous melanoma: An immunoinformatics approach., PMID:38371523

Development of Polyclonal Antibodies-Based Serological Method for the Detection of Calanthe Mild Mosaic Virus and Application in Virus Certification Programme., PMID:38366109

A pipeline for facile cloning of antibody Fv domains and their expression, purification, and characterization as recombinant His-tagged IgGs., PMID:38352391

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-His Tag Antibody (R3Y39) [RGK07905]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only