Catalog No.
RHJ64802
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000
Target
DDX4, VASA, Vasa homolog, DEAD box protein 4, Probable ATP-dependent RNA helicase DDX4
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9NQI0
Applications
FCM, IF, IHC
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3W87
Deletion of ddx4 Ovary-Specific Transcript Causes Dysfunction of Meiosis and Derepress of DNA Transposons in Zebrafish Ovaries., PMID:39765722
In vitro proliferation of Mytilus edulis male germ cell progenitors., PMID:38335194
Germ cell recovery, cryopreservation and transplantation in the California white sturgeon, Acipenser transmontanus., PMID:37803091
Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set., PMID:37077350
Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes., PMID:35052491
The safety of VASApos presumptive adult ovarian stem cells., PMID:34474974
Enhanced method to select human oogonial stem cells for fertility research., PMID:34415395
Differentiation Potential of Cultured Extracellular DEAD-Box Helicase 4+ Oogonial Stem Cells from Adult Human Ovaries into Somatic Lineages., PMID:34412061
Subcellular localization of the mouse PRAMEL1 and PRAMEX1 reveals multifaceted roles in the nucleus and cytoplasm of germ cells during spermatogenesis., PMID:34074333
Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress., PMID:32680554
Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice., PMID:32677505
Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells., PMID:32123174
Integration of IgA and IgG Autoantigens Improves Performance of Biomarker Panels for Early Diagnosis of Lung Cancer., PMID:31924693
Extracellular Localisation of the C-Terminus of DDX4 Confirmed by Immunocytochemistry and Fluorescence-Activated Cell Sorting., PMID:31212843
GDNF family receptor alpha 1 is a reliable marker of undifferentiated germ cells in bulls., PMID:31029848
Photobiomodulation and gametogenic potential of human Wharton's jelly-derived mesenchymal cells., PMID:31029424
Geldanamycin administration reduces the amount of primordial germ cells in the mouse embryo., PMID:29807855
In vitro differentiation of human oocyte-like cells from oogonial stem cells: single-cell isolation and molecular characterization., PMID:29304224
Gonadal morphogenesis and establishment of the germline in the phyllostomid bat Sturnira lilium., PMID:28823522
VASA mRNA (DDX4) detection is more specific than immunohistochemistry using poly- or monoclonal antibodies for germ cells in the male urogenital tract., PMID:28746190
PL10 DEAD-Box Protein is Expressed during Germ Cell Differentiation in the Reptile Podarcis sicula (Family Lacertidae)., PMID:28656658
Localization and distribution of gonadal proteins in the oviparous lizard Sceloporus aeneus (Squamata: Phrynosomatidae)., PMID:28515008
Identification of Germ-Line Stem Cells in Zebrafish., PMID:27734351
Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence-Activated Cell Sorting., PMID:27557587
FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells., PMID:27301892
Production of offspring from a germline stem cell line derived from prepubertal ovaries of germline reporter mice., PMID:27141102
VASA (DDX4) is a Putative Marker for Spermatogonia, Spermatocytes and Round Spermatids in Stallions., PMID:26482643
In vitro generation and characterization of chicken long-term germ cells from different embryonic origins., PMID:26037665
Isolation and transplantation of sturgeon early-stage germ cells., PMID:25559841
Mapping novel immunogenic epitopes in IgA nephropathy., PMID:25542908
Isolation of chromatoid bodies from mouse testis as a rich source of short RNAs., PMID:24920356
Transfer and detection of freshly isolated or cultured chicken (Gallus gallus) and exotic species' embryonic gonadal germ stem cells in host embryos., PMID:24882096
In mouse oocytes the mitochondrion-originated germinal body-like structures accumulate mouse Vasa homologue (MVH) protein., PMID:24780413
Immunofluorescent analysis of testicular biopsies with germ cell and Sertoli cell markers shows significant MVH negative germ cell depletion with older age at orchiopexy., PMID:24012581
Improved efficiency of female germline stem cell purification using fragilis-based magnetic bead sorting., PMID:21615296
Localization of mouse vasa homolog protein in chromatoid body and related nuage structures of mammalian spermatogenic cells during spermatogenesis., PMID:20401665
Arginine methylation of vasa protein is conserved across phyla., PMID:20080973
A marsupial, Trichosurus vulpecula, DDX4/VASAGene (TvDDX4) of the DEAD box protein family: molecular conservation and germline expression., PMID:20016130
Fetal testis dysgenesis and compromised Leydig cell function in Tgfbr3 (beta glycan) knockout mice., PMID:19696014
[Cloning, prokaryotic expression of rat RVLG and preparation of mouse anti-RVLG polyclonal antibody]., PMID:19256349
Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia., PMID:17486274
Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: domain composition, intracellular localization, and function in male germ cells in mice., PMID:17141210
A novel cyclic peptide immunization strategy for preventing HIV-1/AIDS infection and progression., PMID:12771150
The human VASA gene is specifically expressed in the germ cell lineage., PMID:10920202
Vasa protein expression and localization in the zebrafish., PMID:10906476
Involvement of the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) in the differentiation of primordial germ cells., PMID:9338598
Isolation and characterization of a novel gene of the DEAD box protein family which is specifically expressed in germ cells of Xenopus laevis., PMID:8150200
For research use only. Not for human or drug use.
