Catalog No.
RHJ56805
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000
Target
Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A, MAP1A/MAP1B light chain 3 A
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9H492
Applications
ELISA, FCM, IHC
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3W49
Autophagy Blockage Up-Regulates HLA-Class-I Molecule Expression in Lung Cancer and Enhances Anti-PD-L1 Immunotherapy Efficacy., PMID:39409895
Assessment of Non-canonical Functions of the Autophagy Proteins in LC3-Associated Phagocytosis and LC3-Associated Endocytosis., PMID:39046619
The Role of LC3-Associated Phagocytosis Inhibits the Inflammatory Response in Aspergillus fumigatus Keratitis., PMID:38953845
Does Autophagy have a Role in the Pathogenesis of Pediatric Hepatic Steatosis?, PMID:38809648
Immunohistochemical analysis with apoptosis and autophagy markers in periodontitis and peri-implantitis: Clinical comparative study., PMID:36755315
Belimumab Decreases Autophagy and Citrullination in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus., PMID:35053379
LC3/FtMt Colocalization Patterns Reveal the Progression of FtMt Accumulation in Nigral Neurons of Patients with Progressive Supranuclear Palsy., PMID:35008961
Autophagic secretion of HMGB1 from cancer-associated fibroblasts promotes metastatic potential of non-small cell lung cancer cells via NFκB signaling., PMID:34552063
Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy., PMID:34542166
Cyclovirobuxine D Induced-Mitophagy through the p65/BNIP3/LC3 Axis Potentiates Its Apoptosis-Inducing Effects in Lung Cancer Cells., PMID:34072333
The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore., PMID:33783314
Integrated Transcriptomic and Epigenetic Study of PCOS: Impact of Map3k1 and Map1lc3a Promoter Methylation on Autophagy., PMID:33763111
Interleukin‑8 released by cancer‑associated fibroblasts attenuates the autophagy and promotes the migration of ovarian cancer cells., PMID:33649784
Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival., PMID:33526872
Autoantibodies against the autophagic protein microtubule-associated light-chain 3 (LC3): Immunocharacterization of an atypical ANA pattern., PMID:32338081
Co-expression of Hsp70 Protein and Autophagy Marker Protein LC3 in A549 Cells and THP1 Cells Exposed to Nanoparticles of Air Pollution., PMID:31925751
[Increased expression of LC3 in peripheral blood mononuclear cells is related to disease status in patients with rheumatoid arthritis]., PMID:31879000
[Serine hydroxymethyl transferase 2 regulates the AMPK/mTOR pathway and induces autophagy to promote chemotherapy resistance in colon cancer cells]., PMID:31167694
Predictive and prognostic implications of 4E-BP1, Beclin-1, and LC3 for cetuximab treatment combined with chemotherapy in advanced colorectal cancer with wild-type KRAS: Analysis from real-world data., PMID:31057298
Tumor autophagy is associated with survival outcomes in patients with resected non-small cell lung cancer., PMID:30797498
Methods to Measure Autophagy in Cancer Metabolism., PMID:30725456
Characterization of the "Autophagic Flux" in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy., PMID:30610722
LC3A, LC3B and Beclin-1 Expression in Gastric Cancer., PMID:30504397
Enhanced neutrophil autophagy and increased concentrations of IL-6, IL-8, IL-10 and MCP-1 in rheumatoid arthritis., PMID:30312880
MAP1A/BLC3? Now I am really confused., PMID:30296899
Deregulation of autophagy under hyperglycemic conditions is dependent on increased lysine 63 ubiquitination: a candidate mechanism in the progression of diabetic nephropathy., PMID:29806072
Human cytomegalovirus infection-induced autophagy was associated with the biological behavioral changes of human umbilical vein endothelial cell (HUVEC)., PMID:29710549
Lithium chloride (LiCl) induced autophagy and downregulated expression of transforming growth factor β-induced protein (TGFBI) in granular corneal dystrophy., PMID:29679546
The Effect of and Mechanism Underlying Autophagy in Hepatocellular Carcinoma Induced by CH12, a Monoclonal Antibody Directed Against Epidermal Growth Factor Receptor Variant III., PMID:29587298
Cardiolipin exposure on the outer mitochondrial membrane modulates α-synuclein., PMID:29483518
Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain., PMID:28984607
IL-12 induces autophagy in human breast cancer cells through AMPK and the PI3K/Akt pathway., PMID:28765958
Imiquimod-induced autophagy is regulated by ER stress-mediated PKR activation in cancer cells., PMID:28601430
Bioluminescence Resonance Energy Transfer (BRET)-Based Synthetic Sensor Platform for Drug Discovery., PMID:28369669
Association of podocyte autophagosome numbers with idiopathic membranous nephropathy and secondary membranous nephropathy., PMID:28285376
Inhibition of Inositol 1, 4, 5-Trisphosphate Receptor Induce Breast Cancer Cell Death Through Deregulated Autophagy and Cellular Bioenergetics., PMID:28106298
Autophagy and ubiquitin-proteasome system contribute to sperm mitophagy after mammalian fertilization., PMID:27551072
Neutrophil gelatinase-associated lipocalin worsens ischemia/reperfusion damage of kidney cells by autophagy., PMID:27380103
Quantifying autophagy: Measuring LC3 puncta and autolysosome formation in cells using multispectral imaging flow cytometry., PMID:27263026
Autophagy Induced by Intracellular Infection of Propionibacterium acnes., PMID:27219015
ADAM28 is expressed by epithelial cells in human normal tissues and protects from C1q-induced cell death., PMID:26918856
Mechanisms for autophagy modulation by isoprenoid biosynthetic pathway inhibitors in multiple myeloma cells., PMID:26595805
Inhibition of autophagy induced by PTEN loss promotes intrinsic breast cancer resistance to trastuzumab therapy., PMID:26563373
Overexpression of LC3 in Papillary Thyroid Carcinomas and Lymph Node Metastases., PMID:26560002
Autophagy is upregulated during colorectal carcinogenesis, and in DNA microsatellite stable carcinomas., PMID:26502823
Knockdown of AKT3 and PI3KCA by RNA interference changes the expression of the genes that are related to apoptosis and autophagy in T98G glioblastoma multiforme cells., PMID:26481529
Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines., PMID:26378792
Studying Different Binding and Intracellular Delivery Efficiency of ssDNA Single-Walled Carbon Nanotubes and Their Effects on LC3-Related Autophagy in Renal Mesangial Cells via miRNA-382., PMID:26327220
Autophagy inhibition enhances isorhamnetin‑induced mitochondria‑dependent apoptosis in non‑small cell lung cancer cells., PMID:26238746
Reliable LC3 and p62 autophagy marker detection in formalin fixed paraffin embedded human tissue by immunohistochemistry., PMID:26150155
For research use only. Not for human or drug use.
