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Anti-CD273/PD-L2/PDCD1LG2 Antibody (R3W15)

Catalog #:   RHJ48504 Specific References (19) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, IF, WB
Accession: Q9BQ51
Overview

Catalog No.

RHJ48504

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

PDCD1 ligand 2, B7-DC, PD-L2, PDCD1LG2, PDCD1L2, PDL2, Butyrophilin B7-DC, Programmed death ligand 2, Programmed cell death 1 ligand 2, CD273, B7DC, PD-1 ligand 2

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9BQ51

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3W15

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using CD273 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using CD273 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of K562 cells using CD273 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using CD273 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using CD273 mouse mAb against HepG2 (1), Hela (2),NIH/3T3 (3),PC-12 (4),K562 (5),Jurkat (6),MCF-7 (7), and HEK293-6e (8) cell lysate.
  • Western blot
    Western blot analysis using CD273 mouse mAb against HepG2 (1), Hela (2),NIH/3T3 (3),PC-12 (4),K562 (5),Jurkat (6),MCF-7 (7), and HEK293-6e (8) cell lysate.
References

High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood., PMID:36289665

Detrimental Effect of Trypanosoma brucei brucei Infection on Memory B Cells and Host Ability to Recall Protective B-cell Responses., PMID:35363871

Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells., PMID:33631789

Peptide Blocking CTLA-4 and B7-1 Interaction., PMID:33419027

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions., PMID:32324337

The Controversial Role of PD-1 and Its Ligands in Gynecological Malignancies., PMID:31681606

A role for B cells in organic dust induced lung inflammation., PMID:29273051

CD73 expression identifies a subset of IgM+ antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent., PMID:28746783

PD-1 function in apoptosis of T lymphocytes in canine visceral leishmaniasis., PMID:27016050

Cutting Edge: CTLA-4Ig Inhibits Memory B Cell Responses and Promotes Allograft Survival in Sensitized Recipients., PMID:26416270

Antagonist antibodies to PD-1 and B7-H1 (PD-L1) in the treatment of advanced human cancer., PMID:23460533

Memory B cells in the lung participate in protective humoral immune responses to pulmonary influenza virus reinfection., PMID:22308386

Aging-associated B7-DC+ B cells enhance anti-tumor immunity via Th1 and Th17 induction., PMID:22044484

Cutting edge: Hierarchy of maturity of murine memory B cell subsets., PMID:21078902

New monoclonal antibodies against B-cell antigens: possible new strategies for diagnosis of primary cutaneous B-cell lymphomas., PMID:20951741

Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line., PMID:20628373

B7-H1 on myeloid-derived suppressor cells in immune suppression by a mouse model of ovarian cancer., PMID:18790673

"Alternatively activated" dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig., PMID:17056511

Migratory Langerhans cells in mouse lymph nodes in steady state and inflammation., PMID:15982311

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-CD273/PD-L2/PDCD1LG2 Antibody (R3W15) [RHJ48504]
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