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Anti-SMAD5 Antibody (R3W07)

Catalog #:   RHJ46102 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IF, IHC, WB
Accession: Q99717
Overview

Catalog No.

RHJ46102

Species reactivity

Human, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

SMAD 5, Mothers against decapentaplegic homolog 5, SMAD5, Mothers against DPP homolog 5, Smad5, SMAD family member 5, MAD homolog 5, JV5-1, MADH5, hSmad5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q99717

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3W07

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
References

A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling., PMID:39196179

EpicTope: narrating protein sequence features to identify non-disruptive epitope tagging sites., PMID:38559275

Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF-κB Signaling Pathway following Stimulation of the Inflammatory Microenvironment., PMID:35530413

Predictive model based on gene and laboratory data for intravenous immunoglobulin resistance in Kawasaki disease in a Chinese population., PMID:34174887

Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival., PMID:34013359

Up-regulation of miR-155 potentiates CD34+ CML stem/progenitor cells to escape from the growth-inhibitory effects of TGF-ß1 and BMP signaling., PMID:33907541

Impact of vaccine type on HIV-1 vaccine elicited antibody durability and B cell gene signature., PMID:32747654

Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections., PMID:30414134

Erythroferrone inhibits the induction of hepcidin by BMP6., PMID:30097509

Autoantibodies targeting TLR and SMAD pathways define new subgroups in systemic lupus erythematosus., PMID:29576246

Activin A amplifies dysregulated BMP signaling and induces chondro-osseous differentiation of primary connective tissue progenitor cells in patients with fibrodysplasia ossificans progressiva (FOP)., PMID:29170109

Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6., PMID:28815688

Transforming growth factor-β stimulates Smad1/5 signaling in pulmonary artery smooth muscle cells and fibroblasts of the newborn mouse through ALK1., PMID:28642261

BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5., PMID:28489883

Activation of CD137 Signaling Promotes Angiogenesis in Atherosclerosis via Modulating Endothelial Smad1/5-NFATc1 Pathway., PMID:28288971

Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma., PMID:28199984

The SMAD Pathway Is Required for Hepcidin Response During Endoplasmic Reticulum Stress., PMID:27483343

Partial Müllerian Duct Retention in Smad4 Conditional Mutant Male Mice., PMID:27194944

Bone Morphogenic Protein 4-Smad-Induced Upregulation of Platelet-Derived Growth Factor AA Impairs Endothelial Function., PMID:26769046

Therapeutic potential of regulatory cytokines that target B cells., PMID:26647406

Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling., PMID:26071935

Hyaluronan inhibits BMP-induced osteoblast differentiation., PMID:25592835

Targeting eIF4GI translation initiation factor affords an attractive therapeutic strategy in multiple myeloma., PMID:24815186

The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain., PMID:24495875

MicroRNA-155 controls RB phosphorylation in normal and malignant B lymphocytes via the noncanonical TGF-β1/SMAD5 signaling module., PMID:24136167

SnoN facilitates ALK1-Smad1/5 signaling during embryonic angiogenesis., PMID:24019535

Spatio-temporal distribution of Smads and role of Smads/TGF-β/BMP-4 in the regulation of mouse bladder organogenesis., PMID:23620745

TGF-β1, Smad-2/-3, Smad-1/-5/-8, and Smad-4 signaling factors are expressed in ameloblastomas, adenomatoid odontogenic tumors, and calcifying cystic odontogenic tumors: an immunohistochemical study., PMID:23157422

Sensing BMP pathway activity by immune detection of phosphorylated R-Smad proteins in mouse embryonic kidney., PMID:22639269

Chondroitin sulfate-E fine-tunes osteoblast differentiation via ERK1/2, Smad3 and Smad1/5/8 signaling by binding to N-cadherin and cadherin-11., PMID:22440395

Smad1/Smad5 signaling in limb ectoderm functions redundantly and is required for interdigital programmed cell death., PMID:22240098

Smad 1/5 is involved in bone morphogenetic protein-2-induced odontoblastic differentiation in human dental pulp cells., PMID:22152623

Hic-5 controls BMP4 responses in prostate cancer cells through interacting with Smads 1, 5 and 8., PMID:21996749

Dynamic expression of bone morphogenetic protein 4 in reproductive organs of female mice., PMID:21810858

Loss of Smad5 leads to the disassembly of the apical junctional complex and increased susceptibility to experimental colitis., PMID:21212325

CRIM1 is expressed at higher levels in drug-resistant than in drug-sensitive myeloid leukemia HL60 cells., PMID:21036735

Galectin-9 induces osteoblast differentiation through the CD44/Smad signaling pathway., PMID:20206131

Human adipose-derived stromal cells respond to and elaborate bone morphogenetic protein-2 during in vitro osteogenic differentiation., PMID:20124834

Evidence for the prevention of enthesitis in HLA-B27/hβ(2)m transgenic rats treated with a monoclonal antibody against TNF-α., PMID:20015205

Characterization of mammary epithelial cell line HC11 using the NIA 15k gene array reveals potential regulators of the undifferentiated and differentiated phenotypes., PMID:19523745

Real time monitoring of BMP Smads transcriptional activity during mouse development., PMID:18615729

Mechanisms of murine lacrimal gland repair after experimentally induced inflammation., PMID:18586880

Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad., PMID:18310319

Detection, identification and in vivo treatment responsiveness of bone morphogenetic protein (BMP)-activated cell populations in the synovium of patients with rheumatoid arthritis., PMID:18276742

Development of a rabbit monoclonal antibody group against Smads and immunocytochemical study of human and mouse embryonic stem cells., PMID:18158783

SMAD expression in the testis: an insight into BMP regulation of spermatogenesis., PMID:18069690

Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells., PMID:17962458

Bone morphogenetic protein signaling pathway plays multiple roles during gastrointestinal tract development., PMID:16110505

BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1., PMID:15877825

Target validation for genomics using peptide-specific phage antibodies: a study of five gene products overexpressed in colorectal cancer., PMID:12209988

Datasheet

Document Download

Anti-SMAD5 Antibody (R3W07).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SMAD5 Antibody (R3W07) [RHJ46102]
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