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Anti-MAP3K5 Antibody (R3W04)

Catalog #:   RHJ45802 Specific References (30) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC
Accession: Q99683
Overview

Catalog No.

RHJ45802

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000

Target

ASK1, MAPK/ERK kinase kinase 5, MEKK5, MAPKKK5, MEKK 5, Apoptosis signal-regulating kinase 1, ASK-1, Mitogen-activated protein kinase kinase kinase 5, MAP3K5, MEK kinase 5

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q99683

Applications

ELISA, FCM, IF, IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3W04

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.
References

Titanium Dioxide Nanoparticles Exacerbate Allergic Airway Inflammation via TXNIP Upregulation in a Mouse Model of Asthma., PMID:34576095

Selonsertib (GS-4997), an ASK1 inhibitor, antagonizes multidrug resistance in ABCB1- and ABCG2-overexpressing cancer cells., PMID:30315846

Killing of Latently HIV-Infected CD4 T Cells by Autologous CD8 T Cells Is Modulated by Nef., PMID:30254642

A novel synthetic analogue of ω-3 17,18-epoxyeicosatetraenoic acid activates TNF receptor-1/ASK1/JNK signaling to promote apoptosis in human breast cancer cells., PMID:28798154

MPTP activates ASK1-p38 MAPK signaling pathway through TNF-dependent Trx1 oxidation in parkinsonism mouse model., PMID:26164633

Atmospheric gas plasma-induced ROS production activates TNF-ASK1 pathway for the induction of melanoma cancer cell apoptosis., PMID:24574456

Novel small molecule inhibitors of TLR7 and TLR9: mechanism of action and efficacy in vivo., PMID:24342772

Apoptosis signal-regulating kinase 1 is involved in WISP-1-promoted cell motility in human oral squamous cell carcinoma cells., PMID:24205072

Effects of butyltins on mitogen-activated-protein kinase kinase kinase and Ras activity in human natural killer cells., PMID:24038145

Requirement of apoptosis-inducing kinase 1 for the induction of bronchial asthma following stimulation with ovalbumin., PMID:23921222

CTGF increases IL-6 expression in human synovial fibroblasts through integrin-dependent signaling pathway., PMID:23227240

Stress-induced nuclear import of apoptosis signal-regulating kinase 1 is mediated by karyopherin α2/β1 heterodimer., PMID:23123190

ASK1 promotes apoptosis of normal and malignant plasma cells., PMID:22723553

Apoptosis signal-regulating kinase 1 is mediated in TNF-α-induced CCL2 expression in human synovial fibroblasts., PMID:22711527

Potential characteristics of stem cells from human exfoliated deciduous teeth compared with bone marrow-derived mesenchymal stem cells for mineralized tissue-forming cell biology., PMID:22099898

Arginine methylation-dependent regulation of ASK1 signaling by PRMT1., PMID:22095282

Apoptosis signal-regulating kinase (ASK)-1 mediates apoptosis through activation of JNK1 following engagement of membrane immunoglobulin., PMID:19766627

Inhibition of apoptosis signal-regulating kinase 1 reduces endoplasmic reticulum stress and nuclear huntingtin fragments in a mouse model of Huntington disease., PMID:19646509

Lipopolysaccharide triggered TNF-alpha-induced hepatocyte apoptosis in a murine non-alcoholic steatohepatitis model., PMID:19446916

Overexpression of REIC/Dkk-3 in normal fibroblasts suppresses tumor growth via induction of interleukin-7., PMID:19279003

4-Hydroxynonenal self-limits fas-mediated DISC-independent apoptosis by promoting export of Daxx from the nucleus to the cytosol and its binding to Fas., PMID:18069800

RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation., PMID:17389591

Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3., PMID:16540654

15S-Lipoxygenase-2 mediates arachidonic acid-stimulated adhesion of human breast carcinoma cells through the activation of TAK1, MKK6, and p38 MAPK., PMID:16000313

Important role of apoptosis signal-regulating kinase 1 in ischemia-induced angiogenesis., PMID:15976330

Direct binding of apoptosis signal-regulating kinase 1 to retinoblastoma protein: novel links between apoptotic signaling and cell cycle machinery., PMID:15210709

The cytoplasmic domain of Alzheimer's amyloid-beta protein precursor causes sustained apoptosis signal-regulating kinase 1/c-Jun NH2-terminal kinase-mediated neurotoxic signal via dimerization., PMID:12829723

Formation of a tumour necrosis factor receptor 1 molecular scaffolding complex and activation of apoptosis signal-regulating kinase 1 during seizure-induced neuronal death., PMID:12786973

The role of apoptosis signal-regulating kinase 1 in lymphotoxin-beta receptor-mediated cell death., PMID:12566458

Double-stranded RNA-activated protein kinase interacts with apoptosis signal-regulating kinase 1. Implications for apoptosis signaling pathways., PMID:12473108

Datasheet

Document Download

Anti-MAP3K5 Antibody (R3W04).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-MAP3K5 Antibody (R3W04) [RHJ45802]
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