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Anti-RAD50 Antibody (R3V64)

Catalog #:   RHJ34202 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: ELISA, FCM, IHC, WB
Accession: Q92878
Overview

Catalog No.

RHJ34202

Species reactivity

Human, Rat

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

RAD50, hRAD50, DNA repair protein RAD50

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q92878

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3V64

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using RAD50 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using RAD50 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using RAD50 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using RAD50 mouse mAb against C6 (1) and HepG2 (2) cell lysate.
References

SingleNucleotide Polymorphisms as Biomarkers of Mepolizumab and Benralizumab Treatment Response in Severe Eosinophilic Asthma., PMID:39125709

Genomic analysis of plasma circulating tumor DNA in patients with heavily pretreated HER2 + metastatic breast cancer., PMID:37336919

Topological Analysis of γH2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair., PMID:34771723

MRE11 promotes oral cancer progression through RUNX2/CXCR4/AKT/FOXA2 signaling in a nuclease-independent manner., PMID:33927349

Precision medicine phase II study evaluating the efficacy of a double immunotherapy by durvalumab and tremelimumab combined with olaparib in patients with solid cancers and carriers of homologous recombination repair genes mutation in response or stable after olaparib treatment., PMID:32778095

Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers., PMID:31473966

Localization Microscopy Analyses of MRE11 Clusters in 3D-Conserved Cell Nuclei of Different Cell Lines., PMID:29361783

EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork-Associated DNA Damage Repair., PMID:28801308

Panel of SEREX-defined antigens for breast cancer autoantibodies profile detection., PMID:27775439

DNA damage precedes apoptosis during the regression of the interdigital tissue in vertebrate embryos., PMID:27752097

Polymorphisms of RAD50, IL33 and IL1RL1 are associated with atopic asthma in Chinese population., PMID:26493291

RAD50 Single-Nucleotide Polymorphism in Predominantly Antibody Deficiency., PMID:26310047

Targeting of β1 integrins impairs DNA repair for radiosensitization of head and neck cancer cells., PMID:26073085

Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures., PMID:26004008

Identification of Plasmodium falciparum DNA Repair Protein Mre11 with an Evolutionarily Conserved Nuclease Function., PMID:25938776

Microtubule-targeting agents augment the toxicity of DNA-damaging agents by disrupting intracellular trafficking of DNA repair proteins., PMID:25605897

Identification of the interactors of human nibrin (NBN) and of its 26 kDa and 70 kDa fragments arising from the NBN 657del5 founder mutation., PMID:25485873

The human SRCAP chromatin remodeling complex promotes DNA-end resection., PMID:25176633

Association of BLM and BRCA1 during Telomere Maintenance in ALT Cells., PMID:25084169

Smad7 enhances ATM activity by facilitating the interaction between ATM and Mre11-Rad50-Nbs1 complex in DNA double-strand break repair., PMID:25063542

Fine-mapping of IgE-associated loci 1q23, 5q31, and 12q13 using 1000 Genomes Project data., PMID:24930997

A polymorphism in the TH 2 locus control region is associated with changes in DNA methylation and gene expression., PMID:24866380

Sym004, a novel anti-EGFR antibody mixture, augments radiation response in human lung and head and neck cancers., PMID:24130052

Human candidate polymorphisms in sympatric ethnic groups differing in malaria susceptibility in Mali., PMID:24098393

Multicolor laser scanning confocal immunofluorescence microscopy of DNA damage response biomarkers., PMID:23896884

Ionizing-radiation induced DNA double-strand breaks: a direct and indirect lighting up., PMID:23849169

The MRN-CtIP pathway is required for metaphase chromosome alignment., PMID:23434370

SMC1-mediated intra-S-phase arrest facilitates bocavirus DNA replication., PMID:23365434

Immunohistochemical analysis of medullary breast carcinoma autoantigens in different histological types of breast carcinomas., PMID:23181716

Current concepts of IgE regulation and impact of genetic determinants., PMID:22909159

The Alström syndrome protein, ALMS1, interacts with α-actinin and components of the endosome recycling pathway., PMID:22693585

Activation of protein kinase Tel1 through recognition of protein-bound DNA ends., PMID:21402778

L1CAM regulates DNA damage checkpoint response of glioblastoma stem cells through NBS1., PMID:21297581

Autophosphorylation and ATM activation: additional sites add to the complexity., PMID:21149446

Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex., PMID:20943970

Unique DNA repair gene variations and potential associations with the primary antibody deficiency syndromes IgAD and CVID., PMID:20805886

Loss of juxtaposition of RAG-induced immunoglobulin DNA ends is implicated in the precursor B-cell differentiation defect in NBS patients., PMID:20378756

Temporal regulation of Ig gene diversification revealed by single-cell imaging., PMID:19748985

Multiple functions of MRN in end-joining pathways during isotype class switching., PMID:19633670

Meiotic localization of Mre11 and Rad50 in wild type, spo11-1, and MRN complex mutants of Coprinus cinereus., PMID:19396455

Genetic evidence for single-strand lesions initiating Nbs1-dependent homologous recombination in diversification of Ig v in chicken B lymphocytes., PMID:19180185

Genome-wide scan on total serum IgE levels identifies FCER1A as novel susceptibility locus., PMID:18846228

A tool for enhancement and scoring of DNA repair foci., PMID:18836995

Structure of a second BRCT domain identified in the nijmegen breakage syndrome protein Nbs1 and its function in an MDC1-dependent localization of Nbs1 to DNA damage sites., PMID:18582474

Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolytica., PMID:18402694

Mechanism and regulation of class switch recombination., PMID:18370922

The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius., PMID:18294364

ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain., PMID:17706468

Defective signal joint recombination in fanconi anemia fibroblasts reveals a role for Rad50 in V(D)J recombination., PMID:17524422

Pol eta is required for DNA replication during nucleotide deprivation by hydroxyurea., PMID:17369853

Datasheet

Document Download

Anti-RAD50 Antibody (R3V64).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RAD50 Antibody (R3V64) [RHJ34202]
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