Catalog No.
RHH31302
Species reactivity
Human
Host species
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000
Target
BAK, Bcl2-L-7, BCL2L7, Apoptosis regulator BAK, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, CDN1, BAK1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q16611
Applications
ELISA, FCM, IHC
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3U41
Genomic and Phenotypic Characterization of CHO 4BGD Cells with Quad Knockout and Overexpression of Two Housekeeping Genes That Allow for Metabolic Selection and Extended Fed-Batch Culturing., PMID:40422195
A novel inhibitory BAK antibody enables assessment of non-activated BAK in cancer cells., PMID:38582955
Novel chimeric antigen receptor-expressing T cells targeting the malignant mesothelioma-specific antigen sialylated HEG1., PMID:38212893
A preliminary, prospective study of peripheral neuropathy and cognitive function in patients with breast cancer during taxane therapy., PMID:36206298
Podocytes are lost from glomeruli before completing apoptosis., PMID:36049065
The RECEPTOR-LIKE PROTEIN53 immune complex associates with LLG1 to positively regulate plant immunity., PMID:35796320
Engineering death resistance in CHO cells for improved perfusion culture., PMID:35737825
In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region., PMID:33067389
Evaluation of the upregulation and surface expression of hypoxanthine guanine phosphoribosyltransferase in acute lymphoblastic leukemia and Burkitt's B cell lymphoma., PMID:32782434
Shikonin induces tumor apoptosis in glioma cells via endoplasmic reticulum stress, and Bax/Bak mediated mitochondrial outer membrane permeability., PMID:32663591
Low expression of pro-apoptotic proteins Bax, Bak and Smac indicates prolonged progression-free survival in chemotherapy-treated metastatic melanoma., PMID:32054850
Stable expression of infliximab in CRISPR/Cas9-mediated BAK1-deficient CHO cells., PMID:29915899
Mechanisms of platelet clearance and translation to improve platelet storage., PMID:29475962
Alcoholic hepatitis versus non-alcoholic steatohepatitis: Levels of expression of some proteins involved in tumorigenesis., PMID:29307797
Repression of Mcl-1 and disruption of the Mcl-1/Bak interaction in myeloma cells couple ER stress to mitochondrial apoptosis., PMID:27697610
Identification of an activation site in Bak and mitochondrial Bax triggered by antibodies., PMID:27217060
FBI-1 Is Overexpressed in Gestational Trophoblastic Disease and Promotes Tumor Growth and Cell Aggressiveness of Choriocarcinoma via PI3K/Akt Signaling., PMID:26093985
Mitochondrial p53 phosphorylation induces Bak-mediated and caspase-independent cell death., PMID:25980443
Proapoptotic activities of protein disulfide isomerase (PDI) and PDIA3 protein, a role of the Bcl-2 protein Bak., PMID:25697356
Interaction between Na-K-ATPase and Bcl-2 proteins BclXL and Bak., PMID:25318106
Tumor suppressor protein p53 promotes 2-methoxyestradiol-induced activation of Bak and Bax, leading to mitochondria-dependent apoptosis in human colon cancer HCT116 cells., PMID:25179905
HIV-1 Tat-mediated apoptosis in human blood-retinal barrier-associated cells., PMID:24739951
Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex., PMID:24114786
The novel proteasome inhibitor carfilzomib induces cell cycle arrest, apoptosis and potentiates the anti-tumour activity of chemotherapy in rituximab-resistant lymphoma., PMID:23826755
Prometaphase arrest-dependent phosphorylation of Bcl-2 family proteins and activation of mitochondrial apoptotic pathway are associated with 17α-estradiol-induced apoptosis in human Jurkat T cells., PMID:23707954
The BH3-only protein Bim(L) overrides Bcl-2-mediated apoptosis resistance in melanoma cells., PMID:23402819
Proapoptotic Bak and Bax guard against fatal systemic and organ-specific autoimmune disease., PMID:23349374
The proteasome inhibitor MG132 potentiates TRAIL receptor agonist-induced apoptosis by stabilizing tBid and Bik in human head and neck squamous cell carcinoma cells., PMID:22513214
Histone deacetylase inhibitors induced differentiation and accelerated mineralization of pulp-derived cells., PMID:22341071
Delineation of apoptotic genes for synergistic apoptosis of lexatumumab and anthracyclines in human renal cell carcinoma cells by polymerase chain reaction array., PMID:22205156
Distinct cellular and therapeutic effects of obatoclax in rituximab-sensitive and -resistant lymphomas., PMID:21492126
Blockade of interleukin-6 signalling with siltuximab enhances melphalan cytotoxicity in preclinical models of multiple myeloma., PMID:21241278
Inhibition of MCL-1 in breast cancer cells promotes cell death in vitro and in vivo., PMID:20855960
Fas apoptosis inhibitory molecule regulates T cell receptor-mediated apoptosis of thymocytes by modulating Akt activation and Nur77 expression., PMID:20178987
The glycoprotein Ibalpha-von Willebrand factor interaction induces platelet apoptosis., PMID:19840363
BEX2 regulates mitochondrial apoptosis and G1 cell cycle in breast cancer., PMID:19711341
The VDAC2-BAK rheostat controls thymocyte survival., PMID:19706873
Deficient activation of Bak and Bax confers resistance to gemtuzumab ozogamicin-induced apoptotic cell death in AML., PMID:19463775
Antiapoptotic small interfering RNA as potent adjuvant of DNA vaccination in a mouse mammary tumor model., PMID:19222350
BH3 mimetic obatoclax enhances TRAIL-mediated apoptosis in human pancreatic cancer cells., PMID:19118042
Switch from type II to I Fas/CD95 death signaling on in vitro culturing of primary hepatocytes., PMID:19003879
Down-regulation of Mcl-1 with antisense technology alters the effect of various cytotoxic agents used in treatment of squamous cell carcinoma of the head and neck., PMID:18497956
Acquired resistance to rituximab is associated with chemotherapy resistance resulting from decreased Bax and Bak expression., PMID:18316580
Histone H1.2 is translocated to mitochondria and associates with Bak in bleomycin-induced apoptotic cells., PMID:17879944
Radiation-induced apoptosis along with local and systemic cytokine elaboration is associated with DC plus radiotherapy-mediated renal cell tumor regression., PMID:17449328
Bax does not have to adopt its final form to drive T cell death., PMID:16651384
Inhibition of staurosporine-induced activation of the proapoptotic multidomain Bcl-2 proteins Bax and Bak by three invasive chlamydial species., PMID:16490255
Bax/Bak-dependent release of DDP/TIMM8a promotes Drp1-mediated mitochondrial fission and mitoptosis during programmed cell death., PMID:16332536
Immunogenetics of primary Sjögren's syndrome in Colombians., PMID:15846589
Bcl-xl and Bak protein expression in human optic nerve axons in eyeballs post-trauma and in the eyes with absolute glaucoma., PMID:15638362
For research use only. Not for human or drug use.
