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Anti-MYLK Antibody (R3T99)

Catalog #:   RHH18202 Specific References (6) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q15746
Overview

Catalog No.

RHH18202

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

MLCK1, smMLCK, MYLK1, KRP, Kinase-related protein, MLCK, MYLK, Myosin light chain kinase, smooth muscle, Telokin

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15746

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3T99

Data Image
  • Flow Cytometry
    Flow cytometric analysis of hela cells using MYLK mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of LNCAP cells using MYLK mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using MYLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder Cancer tissues using MYLK mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using MYLK mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using MYLK mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using MYLK mouse mAb against PC-3 (1), C2C12 (2), and Hela (3) cell lysate.
References

CT45A1-mediated MLC2 (MYL9) phosphorylation promotes natural killer cell resistance and outer cell fate in a cell-in-cell structure, potentiating the progression of microsatellite instability-high colorectal cancer., PMID:39322998

Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential., PMID:34548480

Distinct molecular subtypes of uterine leiomyosarcoma respond differently to chemotherapy treatment., PMID:28893210

Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles., PMID:25637593

MiRNA-155 targets myosin light chain kinase and modulates actin cytoskeleton organization in endothelial cells., PMID:24486510

Non-muscle myosin light chain kinase isoform is a viable molecular target in acute inflammatory lung injury., PMID:20139351

Datasheet

Document Download

Anti-MYLK Antibody (R3T99).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-MYLK Antibody (R3T99) [RHH18202]
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