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Anti-TOMM20 Antibody (R3T69)

Catalog #:   RHH08103 Specific References (27) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC, IP, WB
Accession: Q15388
Overview

Catalog No.

RHH08103

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:100-1:200, IP: 1:100-1:200, WB: 1:500-1:1000

Target

Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, KIAA0016, TOMM20, Outer mitochondrial membrane receptor Tom20

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15388

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3T69

Data Image
  • Immunohistochemical
    IHC-P analysis of kidney tissue by TOMM20 antibody (RHH08103). IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue;Result: Cells in tubules are positively stained at the cytoplasm.
  • Western blot
    Western blot analysis using TOMM20 mouse mAb against mouse brain and rat brain
  • Western blot
    Western blot analysis using TOMM20 mouse mAb against HEK-293 lysate
References

Inactivation of SIAH-1 E3 ligase attenuates Aβ toxicity by suppressing ubiquitin-dependent DVE-1 degradation in Caenorhabditis elegans models of Alzheimer's disease., PMID:40349774

NLRP3 inflammasome inhibits mitophagy during the progression of temporal lobe epilepsy., PMID:40348802

Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826

Tissue imaging reveals disruption of epithelial mitochondrial networks and loss of mitochondria-associated cytochrome-C in inflamed human and murine colon., PMID:36356719

Fibroblast-epithelial metabolic coupling in laryngeal cancer., PMID:36327815

Lens Aquaporin-5 Inserts Into Bovine Fiber Cell Plasma Membranes Via Unconventional Protein Secretion., PMID:35816045

Generation of an Ihh-mKate2-Dre knock-in mouse line., PMID:35765931

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives., PMID:34806709

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses., PMID:32249716

Increased number of mitochondria in capillaries distributed in stroke-like lesions of two patients with MELAS., PMID:31408922

Post-embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based on Heat-Induced Antigen Retrieval., PMID:27515088

Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells., PMID:27510808

Gsk3β and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease., PMID:27503909

Spatial profiles of markers of glycolysis, mitochondria, and proton pumps in a rat glioma suggest coordinated programming for proliferation., PMID:26032618

A reverse Warburg metabolism in oral squamous cell carcinoma is not dependent upon myofibroblasts., PMID:25420473

Immunocytochemical localization of the translocase of the outer mitochondrial membrane (Tom20) in the human cochlea., PMID:23165776

Interaction between the human mitochondrial import receptors Tom20 and Tom70 in vitro suggests a chaperone displacement mechanism., PMID:21771790

TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax., PMID:17096026

Voltage-dependent anion channel localises to the plasma membrane and peripheral but not perinuclear mitochondria., PMID:12698369

Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins., PMID:11913975

Characterization of the novel mitochondrial protein import component, Tom34, in mammalian cells., PMID:10101285

Characterization of the initial steps of precursor import into rat liver mitoplasts., PMID:9756630

Interactions of the human mitochondrial protein import receptor, hTom20, with precursor proteins in vitro reveal pleiotropic specificities and different receptor domain requirements., PMID:9211931

A receptor for the import of proteins into human mitochondria., PMID:8654425

Identification of the human mitochondrial protein import receptor, huMas20p. Complementation of delta mas20 in yeast., PMID:7589431

Datasheet

Document Download

Anti-TOMM20 Antibody (R3T69).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-TOMM20 Antibody (R3T69) [RHH08103]
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