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Anti-WTAP Antibody (R3T43)

Catalog #:   RHG96902 Specific References (16) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q15007
Overview

Catalog No.

RHG96902

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:500, IHC: 1:100-1:500, WB: 1:500-1:2000

Target

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15007

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3T43

Data Image
  • Flow Cytometry
    Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat testicles(B) using WTAP mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A) Rabbit cerebellum(b) using WTAP mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate.
  • Western blot
    Western blot analysis using WTAP mouse mAb against PC-12(1)NIH/3T3(2) NRK(3) C2C12 (4) C6(5) L1210(6) F9(7) COS7(8) CHO3D10(9) cell lysate.
References

Astragalus polysaccharide alleviates IL-13-induced oxidative stress injury in nasal epithelial cells by inhibiting WTAP-mediated FBXW7 m6A modification., PMID:38957784

Prognostic value of comprehensive typing based on m6A and gene cluster in TNBC., PMID:36109402

Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression., PMID:34809621

Bone Marrow Mesenchymal Stem Cells-Derived Exosomal miR-425-5p Inhibits Acute Myeloid Leukemia Cell Proliferation, Apoptosis, Invasion and Migration by Targeting WTAP., PMID:34594112

RNA Demethylase FTO Mediated RNA m6A Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion., PMID:34350169

N6-Methyladenosine-Related RNA Signature Predicting the Prognosis of Ovarian Cancer., PMID:34137363

Chidamide increases the sensitivity of Non-small Cell Lung Cancer to Crizotinib by decreasing c-MET mRNA methylation., PMID:32792859

Decreased Peripheral Blood ALKBH5 Correlates with Markers of Autoimmune Response in Systemic Lupus Erythematosus., PMID:32685056

Epitranscriptomics in liver disease: Basic concepts and therapeutic potential., PMID:32330603

Interactions, localization, and phosphorylation of the m6A generating METTL3-METTL14-WTAP complex., PMID:29348140

Feasible etiology of vanishing testis regarding disturbance of testicular development: histopathological and immunohistochemical evaluation of testicular nubbins., PMID:22247958

Wilms tumor 1 expression in vascular neoplasms and vascular malformations., PMID:21697701

Evaluation of podocyte lesion in patients with diabetic nephropathy: Wilms' tumor-1 protein used as a podocyte marker., PMID:19969384

Wilms tumor gene protein 1 is associated with ovarian cancer metastasis and modulates cell invasion., PMID:18260155

Immunohistochemical expression of Wilms' tumor 1 protein in nephroblastoma., PMID:15648285

[Cellular and humoral immune responses against WT1 products overexpressed in hematopoietic malignancies]., PMID:12229118

Datasheet

Document Download

Anti-WTAP Antibody (R3T43).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-WTAP Antibody (R3T43) [RHG96902]
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