Catalog No.
RHG77402
Species reactivity
Human
Host species
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Tested applications
IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
Calcium-dependent tyrosine kinase, Proline-rich tyrosine kinase 2, FAK2, Related adhesion focal tyrosine kinase, Protein-tyrosine kinase 2-beta, RAFTK, PTK2B, PYK2, Calcium-regulated non-receptor proline-rich tyrosine kinase, CAKB, CAK-beta, Focal adhesion kinase 2, FADK 2, Cell adhesion kinase beta, CADTK
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q14289
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3T20
Comprehensive analysis of hub biomarkers associated with immune and oxidative stress in Hashimoto's thyroiditis., PMID:37543352
Distinct roles of ICOS and CD40L in human T-B cell adhesion and antibody production., PMID:34418679
Roles of Pyk2 in signal transduction after gonadotropin-releasing hormone receptor stimulation., PMID:32984962
Contribution of Pyk2 pathway and reactive oxygen species (ROS) to the anti-cancer effects of eicosapentaenoic acid (EPA) in PC3 prostate cancer cells., PMID:32005121
Effects of CCR7 and Src on invasion and migration of salivary gland tumor., PMID:31115008
Beta1 integrin blockade overcomes doxorubicin resistance in human T-cell acute lymphoblastic leukemia., PMID:31043590
The HIV co-receptor CCR5 regulates osteoclast function., PMID:29263385
CCL18 from ascites promotes ovarian cancer cell migration through proline-rich tyrosine kinase 2 signaling., PMID:27613122
Renal microRNA- and RNA-profiles in progressive chronic kidney disease., PMID:26707063
Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms., PMID:25993291
Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses anabolic gene expression., PMID:24841674
Allogeneic T cells: maestro in the co-ordination of the immune response after hematopoietic stem cell transplantation., PMID:24497556
Development of a coordinated allo T cell and auto B cell response against autosomal PTK2B after allogeneic hematopoietic stem cell transplantation., PMID:24097630
Oxidative stress induced by P2X7 receptor stimulation in murine macrophages is mediated by c-Src/Pyk2 and ERK1/2., PMID:23711511
Protein tyrosine kinase signaling in the mouse oocyte cortex during sperm-egg interactions and anaphase resumption., PMID:23401167
Regulation of osteoclast structure and function by FAK family kinases., PMID:22941736
DOCK8 functions as an adaptor that links TLR-MyD88 signaling to B cell activation., PMID:22581261
Small molecule inhibitors of the Pyk2 and FAK kinases modulate chemoattractant-induced migration, adhesion and Akt activation in follicular and marginal zone B cells., PMID:22507871
Early inflammatory reactions in atherosclerosis are induced by proline-rich tyrosine kinase/reactive oxygen species-mediated release of tumor necrosis factor-alpha and subsequent activation of the p21Cip1/Ets-1/p300 system., PMID:21372295
Hypophosphorylated and inactive Pyk2 associates with paxillin at the microtubule organizing center in hematopoietic cells., PMID:21195757
B cell receptor-induced phosphorylation of Pyk2 and focal adhesion kinase involves integrins and the Rap GTPases and is required for B cell spreading., PMID:19561089
The differential expression of Yersinia pseudotuberculosis adhesins determines the requirement for FAK and/or Pyk2 during bacterial phagocytosis by macrophages., PMID:16987330
The Rap GTPases mediate CXCL13- and sphingosine1-phosphate-induced chemotaxis, adhesion, and Pyk2 tyrosine phosphorylation in B lymphocytes., PMID:16821235
Integrin signaling cascades are operational in adult hippocampal synapses and modulate NMDA receptor physiology., PMID:15857387
(alpha)v(beta)3 integrins and Pyk2 mediate insulin-like growth factor I activation of Src and mitogen-activated protein kinase in 3T3-L1 cells., PMID:15761030
Heparin-disaccharide affects T cells: inhibition of NF-kappaB activation, cell migration, and modulation of intracellular signaling., PMID:15020655
The Rap GTPases regulate integrin-mediated adhesion, cell spreading, actin polymerization, and Pyk2 tyrosine phosphorylation in B lymphocytes., PMID:14701796
Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src, proline-rich tyrosine kinase 2, and growth factor receptors., PMID:14670955
Mechanisms of hepatocyte growth factor-mediated vascular smooth muscle cell migration., PMID:14576199
Activation of focal adhesion kinase by protein kinase C epsilon in neonatal rat ventricular myocytes., PMID:12829427
PYK2 autophosphorylation, but not kinase activity, is necessary for adhesion-induced association with c-Src, osteoclast spreading, and bone resorption., PMID:12514172
Angiotensin II-augmented migration of VSMCs towards PDGF-BB involves Pyk2 and ERK 1/2 activation., PMID:12111044
Cas, Fak and Pyk2 function in diverse signaling cascades to promote Yersinia uptake., PMID:12077360
Vasopressin-mediated mitogenic signaling in intestinal epithelial cells., PMID:11832328
Increased expression of cell adhesion kinase beta in human and rat crescentic glomerulonephritis., PMID:11774117
Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies., PMID:11420674
CD44-initiated cell spreading induces Pyk2 phosphorylation, is mediated by Src family kinases, and is negatively regulated by CD45., PMID:11369760
Absence of marginal zone B cells in Pyk-2-deficient mice defines their role in the humoral response., PMID:10881171
PYK2 is an adhesion kinase in macrophages, localized in podosomes and activated by beta(2)-integrin ligation., PMID:11056520
Cerebral ischemia and seizures induce tyrosine phosphorylation of PYK2 in neurons and microglial cells., PMID:10964954
Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages., PMID:10547366
The interaction of activated integrin lymphocyte function-associated antigen 1 with ligand intercellular adhesion molecule 1 induces activation and redistribution of focal adhesion kinase and proline-rich tyrosine kinase 2 in T lymphocytes., PMID:10359604
An unusual mechanism for ligand antagonism., PMID:9677201
Paxillin phosphorylation and association with Lck and Pyk2 in anti-CD3- or anti-CD45-stimulated T cells., PMID:9488700
Proline-rich tyrosine kinase-2 activation by beta 1 integrin fibronectin receptor cross-linking and association with paxillin in human natural killer cells., PMID:9366396
Cytotoxic T lymphocytes express a beta3 integrin which can induce the phosphorylation of focal adhesion kinase and the related PYK-2., PMID:9022036
Restricted expression of cell adhesion kinase-beta in rat tissues., PMID:9006342
Identification and characterization of a novel related adhesion focal tyrosine kinase (RAFTK) from megakaryocytes and brain., PMID:7499242
For research use only. Not for human or drug use.
