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Anti-TRIM25 Antibody (R3T18)

Catalog #:   RHG77203 Specific References (12) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q14258
Overview

Catalog No.

RHG77203

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:250, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

E3 ubiquitin/ISG15 ligase TRIM25, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, EFP, Ubiquitin/ISG15-conjugating enzyme TRIM25, TRIM25, Zinc finger protein 147, RING finger protein 147, RNF147, ZNF147, RING-type E3 ubiquitin transferase, Estrogen-responsive finger protein

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14258

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3T18

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using TRIM25 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using TRIM25 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.
  • Western blot
    Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.
References

Combined use of immunoreactivities of Efp and ZCCHC3 for predicting prognosis of patients with triple-negative breast cancer., PMID:39936817

Interleukin-22 facilitates the interferon-λ-mediated production of tripartite motif protein 25 to inhibit replication of duck viral hepatitis A virus type 1., PMID:37391858

IFN-I inducible miR-3614-5p targets ADAR1 isoforms and fine tunes innate immune activation., PMID:35935998

TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation., PMID:35266845

Combined Use of Immunoreactivities of RIG-I with Efp/TRIM25 for Predicting Prognosis of Patients With Estrogen Receptor-positive Breast Cancer., PMID:33386231

TLR3 Activation of Hepatic Stellate Cell Line Suppresses HBV Replication in HepG2 Cells., PMID:30619284

Dynamic Modulation of Expression of Lentiviral Restriction Factors in Primary CD4+ T Cells following Simian Immunodeficiency Virus Infection., PMID:28100613

Associations between polymorphisms in the antiviral TRIM genes and measles vaccine immunity., PMID:23416095

Negative role of RIG-I serine 8 phosphorylation in the regulation of interferon-beta production., PMID:20406818

Phosphorylation-mediated negative regulation of RIG-I antiviral activity., PMID:20071582

Expression of estrogen-responsive finger protein (Efp) is associated with advanced disease in human epithelial ovarian cancer., PMID:16140366

Molecular cloning of rat efp: expression and regulation in primary osteoblasts., PMID:10425199

Datasheet

Document Download

Anti-TRIM25 Antibody (R3T18).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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For research use only. Not for human or drug use.

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Anti-TRIM25 Antibody (R3T18) [RHG77203]
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