Catalog No.
RHG57905
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
IHC: 1:200-1:1000
Target
PHAS-I, eIF4E-binding protein 1, EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q13541
Applications
IHC
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3S65
Development and application of a targeted phosphoproteomics method for analysing the mTOR pathway dynamics in zebrafish PAC2 cell line., PMID:40466985
Reduced miR-99a-3p levels in systemic lupus erythematosus may promote B cell proliferation via NCAPG and the PI3K/AKT signaling pathway., PMID:38320572
Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression., PMID:34668631
Amino acids and mechanistic target of rapamycin regulate the fate of live engulfed cells., PMID:34547144
Modulation of the IGF1R-MTOR pathway attenuates motor neuron toxicity of human ALS SOD1G93A astrocytes., PMID:33749521
Bevacizumab Augments the Antitumor Efficacy of Infigratinib in Hepatocellular Carcinoma., PMID:33321903
The mTOR Signal Pathway Is Overactivated in Human Lymphatic Malformations., PMID:31381473
MEK inhibition drives anti-viral defence in RV but not RSV challenged human airway epithelial cells through AKT/p70S6K/4E-BP1 signalling., PMID:31319869
Predictive and prognostic implications of 4E-BP1, Beclin-1, and LC3 for cetuximab treatment combined with chemotherapy in advanced colorectal cancer with wild-type KRAS: Analysis from real-world data., PMID:31057298
Interleukin 6 receptor alpha expression in PMNs isolated from prematurely born neonates: decreased expression is associated with differential mTOR signaling., PMID:30965356
The mTORC1/4E-BP/eIF4E Axis Promotes Antibody Class Switching in B Lymphocytes., PMID:30530594
Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis., PMID:29359350
MET Tyrosine Kinase Inhibition Enhances the Antitumor Efficacy of an HGF Antibody., PMID:28341789
Reciprocal regulation of mTOR complexes in pancreatic islets from humans with type 2 diabetes., PMID:28004151
mTORC1 signalling and eIF4E/4E-BP1 translation initiation factor stoichiometry influence recombinant protein productivity from GS-CHOK1 cells., PMID:27760840
Role of mTOR signaling pathway in the pathogenesis of subependymal giant cell astrocytoma - A study of 28 cases., PMID:27625244
Mitotic protein kinase CDK1 phosphorylation of mRNA translation regulator 4E-BP1 Ser83 may contribute to cell transformation., PMID:27402756
Phenotypically Dormant and Immature Leukaemia Cells Display Increased Ribosomal Protein S6 Phosphorylation., PMID:26985829
Integrated Analysis of PTEN and p4EBP1 Protein Expression as Predictors for pCR in HER2-Positive Breast Cancer., PMID:26758558
Differential expression and biochemical activity of the immune receptor Tim-3 in healthy and malignant human myeloid cells., PMID:26413815
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes., PMID:26257270
Doxorubicin induces apoptosis by targeting Madcam1 and AKT and inhibiting protein translation initiation in hepatocellular carcinoma cells., PMID:26124182
Virus-induced translational arrest through 4EBP1/2-dependent decay of 5'-TOP mRNAs restricts viral infection., PMID:26038567
RPPA-based protein profiling reveals eIF4G overexpression and 4E-BP1 serine 65 phosphorylation as molecular events that correspond with a pro-survival phenotype in chronic lymphocytic leukemia., PMID:25999352
Single-cell mass cytometry reveals intracellular survival/proliferative signaling in FLT3-ITD-mutated AML stem/progenitor cells., PMID:25598437
Translational studies within the TAMRAD randomized GINECO trial: evidence for mTORC1 activation marker as a predictive factor for everolimus efficacy in advanced breast cancer., PMID:25361980
Measles vaccine strains for virotherapy of non-small-cell lung carcinoma., PMID:25157763
Effects of targeting endometrial stromal sarcoma cells via histone deacetylase and PI3K/AKT/mTOR signaling., PMID:24922651
κ-Selenocarrageenan prevents microcystin-LR-induced hepatotoxicity in BALB/c mice., PMID:23811533
Intestinal cell kinase (ICK) promotes activation of mTOR complex 1 (mTORC1) through phosphorylation of Raptor Thr-908., PMID:22356909
Sunitinib in urothelial cancer: clinical, pharmacokinetic, and immunohistochemical study of predictors of response., PMID:21645967
Formation of the eIF4F translation-initiation complex determines sensitivity to anticancer drugs targeting the EGFR and HER2 receptors., PMID:21498638
ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding., PMID:21460630
Activation of mTOR signaling pathway associated with adverse prognostic factors of epithelial ovarian cancer., PMID:21276607
Resistance to discodermolide, a microtubule-stabilizing agent and senescence inducer, is 4E-BP1-dependent., PMID:21173253
Activation of host translational control pathways by a viral developmental switch., PMID:19300492
Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein., PMID:19299511
Galectin-3 stabilizes heterogeneous nuclear ribonucleoprotein Q to maintain proliferation of human colon cancer cells., PMID:19137262
Analysis of complex protein kinase B signalling pathways in human prostate cancer samples., PMID:18476967
Comparison of Akt/mTOR signaling in primary breast tumors and matched distant metastases., PMID:18386830
Thymic stromal-derived lymphopoietin induces proliferation of pre-B leukemia and antagonizes mTOR inhibitors, suggesting a role for interleukin-7Ralpha signaling., PMID:17942929
Dietary polyunsaturated fat that is low in (n-3) and high in (n-6) fatty acids alters the SNARE protein complex and nitrosylation in rat hippocampus., PMID:17634254
Interleukin-8 signaling promotes translational regulation of cyclin D in androgen-independent prostate cancer cells., PMID:17606477
Phosphorylation profiles of protein kinases in alveolar and embryonal rhabdomyosarcoma., PMID:17585318
The Akt/mammalian target of rapamycin signal transduction pathway is activated in high-risk myelodysplastic syndromes and influences cell survival and proliferation., PMID:17483341
Constitutive activation of mTOR signaling pathway in post-transplant lymphoproliferative disorders., PMID:17075574
Phosphorylated 4E binding protein 1: a hallmark of cell signaling that correlates with survival in ovarian cancer., PMID:16983702
Mammalian target of rapamycin inhibition induces cell cycle arrest in diffuse large B cell lymphoma (DLBCL) cells and sensitises DLBCL cells to rituximab., PMID:16856892
Activation of mammalian target of rapamycin (mTOR) by insulin is associated with stimulation of 4EBP1 binding to dimeric mTOR complex 1., PMID:16798736
Translational event mediates differential production of tumor necrosis factor-alpha in hyaluronan-stimulated microglia and macrophages., PMID:16573652
For research use only. Not for human or drug use.
