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Anti-SQSTM1 Antibody (R3S58)

Catalog #:   RHG56101 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q13501
Overview

Catalog No.

RHG56101

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:100-1:200, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

OSIL, EBIAP, Sequestosome-1, Ubiquitin-binding protein p62, ORCA, EBI3-associated protein of 60 kDa, p60, SQSTM1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13501

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3S58

Data Image
  • Flow Cytometry
    Flow cytometric analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of C6 cells using SQSTM1 mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of NIH/3T3 cells using SQSTM1 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Rat cortical tissue using SQSTM1 mouse mAb (Red). Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescence
    Immunofluorescence analysis of Mouse cortical tissue using SQSTM1 mouse mAb (Red). Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescence
    Immunofluorescence analysis of Mouse cortical tissue using SQSTM1 mouse mAb (Red). Blue: DRAQ5 fluorescent DNA dye.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using SQSTM1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse Heart using SQSTM1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat Heart using SQSTM1 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit Heart using SQSTM1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate.
References

Hydroxychloroquine confers placental protection in antiphospholipid Syndrome: Mechanistic insights into autophagy regulation and trophoblast dysfunction., PMID:40393103

Dual roles of CXCR4 (C-X-C motif chemokine receptor 4) in promoting entry of ebolavirus and targeting excessive glycoprotein for reticulophagic degradation to facilitate viral fitness., PMID:40223186

Cleavage of the selective autophagy receptor NBR1 by the PDCoV main protease NSP5 impairs autophagic degradation of the viral envelope protein., PMID:40047225

Spitz Spindle Cell/Reed Nevus With SQSTM1 :: NTRK2 Fusion and Atypical Features in an Older Male Patient: A Case Report and Review of Literature., PMID:39980385

ROCK1 promotes B cell differentiation and proteostasis under stress through the heme-regulated proteins, BACH2 and HRI., PMID:39903532

HSP90 stabilizes visual cycle retinol dehydrogenase 5 in the endoplasmic reticulum by inhibiting its degradation during autophagy., PMID:39725039

The Effects of the oxLDL/β2GPI/anti-β2GPI Complex on Macrophage Autophagy and its Mechanism., PMID:39508636

Avian TRIM13 attenuates antiviral innate immunity by targeting MAVS for autophagic degradation., PMID:39508267

Deciphering melanophagy: role of the PTK2-ITCH-MLANA-OPTN cascade on melanophagy in melanocytes., PMID:39477686

Aloperine Suppresses Cancer Progression by Interacting with VPS4A to Inhibit Autophagosome-lysosome Fusion in NSCLC., PMID:39166458

Immunohistochemical Profile of p62/SQSTM1/Sequestosome-1 in Human Low- and High-Grade Intracranial Meningiomas., PMID:39131899

Cleavage of SQSTM1/p62 by the Zika virus protease NS2B3 prevents autophagic degradation of viral NS3 and NS5 proteins., PMID:39128850

PRRSV utilizes MALT1-regulated autophagy flux to switch virus spread and reserve., PMID:39081059

Hydrogen sulphide reduces renal ischemia-reperfusion injury by enhancing autophagy and reducing oxidative stress., PMID:39075751

The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence., PMID:39006307

CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity., PMID:38869076

Antitumor effects of co-treatment of resveratrol with antitumor drugs in ER- and HER2-positive breast cancer cells are due to induction of apoptosis and modulation of estrogen receptor expression., PMID:38780752

Preparation of polyclonal antibodies to chicken P62 protein and its application in nephropathogenic infectious bronchitis virus-infected chickens., PMID:38768912

A potential strategy for bladder cancer treatment: inhibiting autophagy to enhance antitumor effects of Nectin-4-MMAE., PMID:38664366

Enteric coronavirus nsp2 is a virulence determinant that recruits NBR1 for autophagic targeting of TBK1 to diminish the innate immune response., PMID:38597182

Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826

ROS-induced oxidative stress is a major contributor to sperm cryoinjury., PMID:38011909

Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868

Denosumab Induces Neoplastic Stromal Cell Apoptosis Via p62 Downregulation Dependent on Autophagy Pathway in Giant Cell Tumour of Bone., PMID:37961860

Bardoxolone methyl inhibits the infection of rabies virus via Nrf2 pathway activation in vitro., PMID:37950261

Synthetic autophagy receptor., PMID:37934826

Tumor-derived lactate promotes resistance to bevacizumab treatment by facilitating autophagy enhancer protein RUBCNL expression through histone H3 lysine 18 lactylation (H3K18la) in colorectal cancer., PMID:37615625

ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneurons., PMID:37534581

Erianin alleviated liver steatosis by enhancing Nrf2-mediated VE-cadherin expression in vascular endothelium., PMID:37094711

A Case Report of Bone Paget's Disease with Concurrent Psoriasis and Positive Celiac Serology., PMID:37018522

Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1/p62-dependent selective autophagy to antagonize its antiviral activity., PMID:36779599

Proteomic examination of Cornus officinalis stimulated 1.1B4 human pancreatic cells reveals activation of autophagy and Keap1/Nrf2 pathway., PMID:36100124

Autophagy Protects against Eosinophil Cytolysis and Release of DNA., PMID:35681515

Central role for p62/SQSTM1 in the elimination of toxic tau species in a mouse model of tauopathy., PMID:35662390

Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis., PMID:35491858

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD., PMID:35473441

An optimized protocol for immuno-electron microscopy of endogenous LC3., PMID:35387562

Involvement of muscle satellite cell dysfunction in neuromuscular disorders: Expanding the portfolio of satellite cell-opathies., PMID:35302338

TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation., PMID:35266845

Dual roles of p62/SQSTM1 in the injury and recovery phases of acetaminophen-induced liver injury in mice., PMID:35024307

Analysis of Autophagic Vesicles in Mitotic Cells., PMID:34972990

Effects of in vitro vitamin D treatment on function of T cells and autophagy mechanisms in high-fat diet-induced obese mice., PMID:34858547

Ginkgo biloba leaf extract (EGb-761) elicits neuroprotection against cerebral ischemia/reperfusion injury by enhancement of autophagy flux in neurons in the penumbra., PMID:34804431

P62 accumulates through neuroanatomical circuits in response to tauopathy propagation., PMID:34727983

Single-domain antibody screening by isPLA-seq., PMID:34675071

An Autophagy Modulator Peptide Prevents Lung Function Decrease and Corrects Established Inflammation in Murine Models of Airway Allergy., PMID:34572117

Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy., PMID:34572103

Mitochondrial reactive oxygen species trigger metformin-dependent antitumor immunity via activation of Nrf2/mTORC1/p62 axis in tumor-infiltrating CD8T lymphocytes., PMID:34531248

Interaction of Poliovirus Capsid Proteins with the Cellular Autophagy Pathway., PMID:34452452

Autophagy Elicits Neuroprotection at the Subacute Phase of Transient Cerebral Ischaemia but Has Few Effects on Neurological Outcomes After Permanent Ischaemic Stroke in Rats., PMID:34403106

Datasheet

Document Download

Anti-SQSTM1 Antibody (R3S58).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-SQSTM1 Antibody (R3S58) [RHG56101]
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