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Anti-PRKAA1/AMPK alpha 1 Antibody (R3S10)

Catalog #:   RHG41603 Specific References (7) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, IHC, WB
Accession: Q13131
Overview

Catalog No.

RHG41603

Species reactivity

Human, Mouse, Rat, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Tau-protein kinase PRKAA1, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK1, PRKAA1, HMGCR kinase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13131

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3S10

Data Image
  • Flow Cytometry
    Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using PRKAA1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
  • Western blot
    Western blot analysis using PRKAA1 mouse mAb against COS7(1),C2C12(2),NIH/3T3(3) cell lysate.
  • Western blot
    Western blot analysis using PRKAA1 mouse mAb against COS7(1),C2C12(2),NIH/3T3(3) cell lysate.
References

Active vitamin D activates chondrocyte autophagy to reduce osteoarthritis via mediating the AMPK-mTOR signaling pathway., PMID:31815524

Sparc, an EPS-induced gene, modulates the extracellular matrix and mitochondrial function via ILK/AMPK pathways in C2C12 cells., PMID:31150687

TLR1 and PRKAA1 Gene Polymorphisms in the Development of Atrophic Gastritis and Gastric Cancer., PMID:30574617

Anti-inflammatory activities of fenoterol through β-arrestin-2 and inhibition of AMPK and NF-κB activation in AICAR-induced THP-1 cells., PMID:27657826

Protein expression profiling during wallerian degeneration after rat sciatic nerve injury., PMID:24123051

SPARC interacts with AMPK and regulates GLUT4 expression., PMID:20460104

Metabolic and mitogenic signal transduction in human skeletal muscle after intense cycling exercise., PMID:12527721

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-PRKAA1/AMPK alpha 1 Antibody (R3S10) [RHG41603]
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