Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Recombinant Antibody

Anti-NF-κB p65 Antibody (R3Q91)

Catalog #:   RHG01007 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IF, IHC, IP, WB
Accession: Q04206
Overview

Catalog No.

RHG01007

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65, NFKB3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q04206

Applications

IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3Q91

Data Image
  • Immunofluorescence
    Immunofluorescence analysis of NF-KB p65 in Hela using NF-KB p65 antibody,and DAPI (blue).
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded rat Brain Tissue using NF-KB p65 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunoprecipitation
    Immunoprecipitation analysis of NF-KB p65 in Hela lysates using NFκB p65 antibody.
  • Western blot
    Western blot analysis of NF-KB p65 in T47D, 3T3, COS7, C6 and Hela lysates using NF-KB p65 antibody.
  • Western blot
    Western blot analysis of NF-KB p65 in COS7, C6, T47D lysates using NF-KB p65 antibody.
  • Western blot
    Western blot analysis of NF-KB p65 in COS7, C6, T47D lysates using NF-KB p65 antibody.
References

TAP1 promotes immune escape by activating JNK/STAT1/PD-L1 signaling in EBV-associated gastric cancer., PMID:40500531

Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome., PMID:40388674

Buzhong Yiqi decoction improves inflammation and oxidative damage in autoimmune thyroiditis by inhibiting apoptosis via the SIRT1-Mediated Nrf2/NF-κB axis., PMID:40360040

Discovery of novel adjuvants: Identification of saponins from Hylomecon japonica (Thunb.) Prantl & Kündig and insights into their in vitro and in vivo activities., PMID:40318505

PD-1 suppression enhances HIV reactivation and T-cell immunity via MAPK/NF-κB signaling., PMID:40186291

Crude Polygalae Radix after boiling with licorice decoction alleviates intestinal mucosal barrier injury of rats by regulating TLR4/NF-κB signaling pathway., PMID:40120702

LuQi formula ameliorates pressure overload-induced heart failure by regulating macrophages and regulatory T cells., PMID:40118747

Citrullination of NF-κB p65 by PAD2 as a Novel Therapeutic Target for Modulating Macrophage Polarization in Acute Lung Injury., PMID:40087815

Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation., PMID:39931167

DNMT3A transcriptionally downregulated by KLF5 alleviates LPS-induced inflammatory response and promotes osteogenic differentiation in hPDLSCs., PMID:39476105

A Novel Trichinella spiralis Galectin Strengthens the Macrophage ADCC Killing of Larvae via Driving M1 Polarization., PMID:39456703

Chromatin remodelling drives immune cell-fibroblast communication in heart failure., PMID:39443808

Evaluation of Anti-Inflammatory and Immunosuppressant Potential of Isotelekin in Lipopolysaccharide (LPS) Stimulated Macrophage (RAW 264.7) and Sheep Red Blood Cells (SRBC) Sensitized Murine Models., PMID:39410831

Low to moderate ethanol exposure reduces astrocyte-induced neuroinflammatory signaling and cognitive decline in presymptomatic APP/PS1 mice., PMID:39402264

DNp73 enhances tumor progression and immune evasion in multiple myeloma by targeting the MYC and MYCN pathways., PMID:39380995

IL-8 promotes lens capsular residual cells migration by down-regulates expression of E-cadherin and ZO-1 via the CXCR1/2-NF-κB-RhoA signal pathway., PMID:39244903

Based on SIRT1/NF-κB/NLRP3 Signal Pathway to Explore the Effect of Yiqi Huatan Huoxue Recipe on Inflammatory Injury in AIT Mice by Pyroptosis., PMID:39136510

Monocarboxylate transporter 4 facilitates Mycobacterium tuberculosis survival through NF-κB p65-mediated interleukin-10 production., PMID:39108720

The oncogenic kinase TOPK upregulates in psoriatic keratinocytes and contributes to psoriasis progression by regulating neutrophils infiltration., PMID:39090602

Effects of Aeromonas infection on the immune system, physical barriers and microflora structure in the intestine of juvenile grass carp (Ctenopharyngodon idella)., PMID:39059563

DLK1 regulates periodontal inflammation by inhibiting NF-κB p65 and JNK signaling pathways., PMID:38995322

Coexistence of systemic sclerosis and cryopyrin-associated periodic syndrome., PMID:38940218

Effect of IL-9 neutralising antibody on pyroptosis via SGK1/NF-κB/NLRP3/GSDMD in allergic rhinitis mice., PMID:38917753

Inhibition of nucleophosmin/B23 sensitizes ovarian cancer cells to immune check-point blockade via PD-L1 in ovarian cancer., PMID:38821736

Acupuncture at "Die E acupoint" alleviates inflammatory reaction via inhibiting TLR4/MyD88/NF-κB signaling in rats with allergic rhinitis., PMID:38764116

Protective effect of modified Huangqi Chifeng decoction on immunoglobulin A nephropathy through toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B signaling pathway., PMID:38504538

B-cell receptor signaling activity identifies patients with mantle cell lymphoma at higher risk of progression., PMID:38503806

Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway., PMID:38388914

Acupoint application therapy alleviates pain by regulating immune function through inhibiting TLR4/MyD88/NF-κB p65 signaling in a primary dysmenorrhea rat model., PMID:38239137

Exploration of the immuno-adjuvant effect and mechanism of Anemoside B4 through network pharmacology and experiment verification., PMID:38176273

DWN12088, A Prolyl-tRNA Synthetase Inhibitor, Alleviates Hepatic Injury in Nonalcoholic Steatohepatitis., PMID:38173372

Moderate ethanol exposure reduces astrocyte-induced neuroinflammatorysignaling and cognitive decline in presymptomatic APP/PS1 mice., PMID:38077051

Cold-inducible RNA-binding protein induces inflammatory responses via NF-κB signaling pathway in normal human bronchial epithelial cells infected with streptococcus pneumoniae., PMID:38064816

Trichinella spiralis cathepsin L induces macrophage M1 polarization via the NF-κB pathway and enhances the ADCC killing of newborn larvae., PMID:37993938

Nucleoporin downregulation modulates progenitor differentiation independent of nuclear pore numbers., PMID:37853046

DNA-dependent protein kinase catalytic subunit binds to the transactivation domain 1 of NF-κB p65., PMID:37674974

Shikonin prevents mice from heat stroke-induced death via suppressing a trigger IL-17A on the inflammatory and oxidative pathways., PMID:37643485

Anti-cerebral ischemic neuronal injury mechanism of Zhenlong Xingnao capsules: role of the Notch/NF-κB signaling pathway., PMID:37560215

Role of interleukin-36γ induced by ultraviolet radiation in chronic actinic dermatitis., PMID:37545106

[IL-33 up-regulates eIF3a expression by activating NF-κB signaling pathway to mediate the proliferation and differentiation of mouse pulmonary myofibroblasts and aggravate pulmonary fibrosis]., PMID:37515335

Formononetin Inhibits Mast Cell Degranulation to Ameliorate Compound 48/80-Induced Pseudoallergic Reactions., PMID:37446928

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer., PMID:37434399

Pathogenicity and innate immune responses induced by fowl adenovirus serotype 8b in specific pathogen-free chicken., PMID:37354616

Fenofibrate attenuates asthma features in an ovalbumin-induced mouse model via suppressing NF-κB binding activity., PMID:37295485

Rituximab exerts its anti-arthritic effects via inhibiting NF-κB/GM-CSF/iNOS signaling in B cells in a mouse model of collagen-induced arthritis., PMID:37274671

Impact of Toll-like Receptor 4 Expression on Inflammatory Responses Related to Premature Membrane Rupture Induced by Lipopolysaccharide., PMID:37272109

IL-17C neutralization protects the kidney against acute injury and chronic injury., PMID:37263138

Leocarpinolide B Attenuates Collagen Type II-Induced Arthritis by Inhibiting DNA Binding Activity of NF-κB., PMID:37241980

IL-23/IL-17 immune axis mediates the imiquimod-induced psoriatic inflammation by activating ACT1/TRAF6/TAK1/NF-κB pathway in macrophages and keratinocytes., PMID:37098777

Benzene induces spleen injury through the B cell receptor signaling pathway., PMID:37080132

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-NF-κB p65 Antibody (R3Q91) [RHG01007]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only