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Anti-ARF1 Antibody (R3P99)

Catalog #:   RHF75701 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM
Accession: P84077
Overview

Catalog No.

RHF75701

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400

Target

ADP-ribosylation factor 1, ARF1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P84077

Applications

ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3P99

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using ARF1 mouse mAb (green) and negative control (red).
References

The Arf pathway is required for resolving ER stress during T cell activation., PMID:40405457

Partial Protection of Goats against Haemonchus contortus Achieved with ADP-Ribosylation Factor 1 Encapsulated in PLGA Nanoparticles., PMID:39460354

Expression analysis of type I ARF small GTPases ARF1-3 during mouse brain development., PMID:38227057

Dynamin-like Protein 1 (DNML1) as a Molecular Target for Antibody-Based Immunotherapy to Treat Glaucoma., PMID:36362420

Structural and functional analysis of the small GTPase ARF1 reveals a pivotal role of its GTP-binding domain in controlling of the generation of viral inclusion bodies and replication of grass carp reovirus., PMID:36091067

Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells., PMID:30054383

Analysis of Membrane Protein Topology in the Plant Secretory Pathway., PMID:28861819

Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics., PMID:28830537

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense., PMID:28604719

Overexpression of ARF1 is associated with cell proliferation and migration through PI3K signal pathway in ovarian cancer., PMID:28098897

GB virus type C E2 protein inhibits human immunodeficiency virus type 1 Gag assembly by downregulating human ADP-ribosylation factor 1., PMID:26675377

Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion., PMID:26291822

Hematopoietic stem/progenitor cells directly contribute to arteriosclerotic progression via integrin β2., PMID:25546260

Diverse mechanisms regulate the surface expression of immunotherapeutic target ctla-4., PMID:25538704

Generation of unique poliovirus RNA replication organelles., PMID:24570367

Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy., PMID:23804711

ARF1-regulated coatomer directs the steady-state localization of protein kinase C epsilon at the Golgi apparatus., PMID:23195223

The Drosophila Arf1 homologue Arf79F is essential for lamellipodium formation., PMID:22992458

ADP ribosylation factor 1 activity is required to recruit AP-1 to the major histocompatibility complex class I (MHC-I) cytoplasmic tail and disrupt MHC-I trafficking in HIV-1-infected primary T cells., PMID:21917951

ARF1 localizes to the golgi and the trans-golgi network., PMID:21406621

Cdc42 regulates microtubule-dependent Golgi positioning., PMID:20525016

Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster., PMID:20067992

Interaction of brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 1 and kinesin motor protein KIF21A., PMID:19020088

Proteome analysis of Legionella vacuoles purified by magnetic immunoseparation reveals secretory and endosomal GTPases., PMID:18980612

Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin., PMID:18292223

Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association., PMID:17429068

Confocal microscopy-based linescan methodologies for intra-Golgi localization of proteins., PMID:17341478

AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors., PMID:16866877

Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1., PMID:16332543

The role of ARF1 and rab GTPases in polarization of the Golgi stack., PMID:16101683

Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport., PMID:15821732

BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity., PMID:15644318

LdARF1 in trafficking and structural maintenance of the trans-Golgi cisternal network in the protozoan pathogen Leishmania donovani., PMID:15479452

Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1., PMID:15212764

ARF1 regulates Nef-induced CD4 degradation., PMID:15202998

Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors., PMID:14973189

Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini., PMID:14690501

Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1., PMID:12967569

Identification and localization of a beta-COP-like protein involved in the morphodynamics of the plant Golgi apparatus., PMID:12885863

HIV-1 Nef stabilizes the association of adaptor protein complexes with membranes., PMID:12486136

PGF(2alpha)-induced contraction of cat esophageal and lower esophageal sphincter circular smooth muscle., PMID:12121874

Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection., PMID:12006667

GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors., PMID:11950392

Regulation and recruitment of phosphatidylinositol 4-kinase on immature secretory granules is independent of ADP-ribosylation factor 1., PMID:11931656

Site-specific cross-linking reveals a differential direct interaction of class 1, 2, and 3 ADP-ribosylation factors with adaptor protein complexes 1 and 3., PMID:11926829

Association of ARF and Rabs with complement receptor type-1 storage vesicles in human neutrophils., PMID:11590205

The mammalian guanine nucleotide exchange factor mSec12 is essential for activation of the Sar1 GTPase directing endoplasmic reticulum export., PMID:11422940

ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6., PMID:11226253

Aux/IAA proteins are phosphorylated by phytochrome in vitro., PMID:11115889

Separation of membrane trafficking and actin remodeling functions of ARF6 with an effector domain mutant., PMID:10913182

Datasheet

Document Download

Anti-ARF1 Antibody (R3P99).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-ARF1 Antibody (R3P99) [RHF75701]
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