Catalog No.
RHF75509
Species reactivity
Human, Mouse, Rat
Host species
Mouse
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:50-1:100, WB: 1:500-1:1000
Target
Mad3, JV15-2, Mothers against decapentaplegic homolog 3, SMAD family member 3, Smad3, SMAD3, MADH3, hMAD-3, SMAD 3, MAD homolog 3, hSMAD3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 2, SMAD 2, SMAD2, JV18-1, Mad-related protein 2, hMAD-2, MADR2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, Smad2, hSMAD2, MAD homolog 2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P84022,Q15796
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3P97
Activin A exacerbates neonatal necrotizing enterocolitis via ALK4-mediated apoptosis and barrier disruption., PMID:40494204
Eosinophils-Induced Lumican Secretion by Synovial Fibroblasts Alleviates Cartilage Degradation via the TGF-β Pathway Mediated by Anxa1 Binding., PMID:40125663
ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice., PMID:39994804
The Effect of Anti-Activin Receptor Type IIA and Type IIB Antibody on Muscle, Bone and Blood in Healthy and Osteosarcopenic Mice., PMID:39887865
M2 macrophages regulate nucleus pulposus cell extracellular matrix synthesis through the OPN-CD44 axis in intervertebral disc degeneration., PMID:39842659
Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation., PMID:39842382
Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains., PMID:39840816
Atezolizumab plus bevacizumab in patients with unresectable or metastatic mucosal melanoma: 3-year survival update and multi-omics analysis., PMID:39757723
[Effect of Buzhong Yiqi Decoction on Treg cells in rats with autoimmune thyroiditis through TGF-β/Smad signaling pathway]., PMID:39701767
Iron overload in acquired sideroblastic anemias and MDS: pathophysiology and role of chelation and luspatercept., PMID:39644054
A novel mouse model of myositis-associated interstitial lung disease was established by using TLR9 agonist combined with muscle homogenate., PMID:39575634
Allergo-immunopathology mechanism of thymol-inhibiting airway remodeling in asthmatic mice by regulating TGF-β/Smad3 pathway., PMID:39278851
Spike Protein of SARS-CoV-2 Activates Cardiac Fibrogenesis through NLRP3 Inflammasomes and NF-κB Signaling., PMID:39195221
Losartan alters osteoblast differentiation and increases bone mass through inhibition of TGFB signalling in vitro and in an OIM mouse model., PMID:39185375
CD5L up-regulates the TGF-β signaling pathway and promotes renal fibrosis., PMID:39127319
Analysis of the DNA-binding properties of TGF-β-activated Smad complexes unveils a possible molecular basis for cellular context-dependent signaling., PMID:39114961
Rutin reduces inflammation and fibrosis via TGF-β/SMAD pathways in IgA nephropathy induced rats., PMID:39113164
Adipose-derived mesenchymal stromal cells alleviate intestinal fibrosis: The role of tumor necrosis factor-stimulated gene 6 protein., PMID:39024752
Increased EHD1 in trophoblasts causes RSM by activating TGFβ signaling†., PMID:39012723
PAR2 activation on human tubular epithelial cells engages converging signaling pathways to induce an inflammatory and fibrotic milieu., PMID:39005931
Lrba participates in the differentiation of IgA+ B lymphocytes through TGFβR signaling., PMID:38975349
Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis., PMID:38698265
ID1high/activin Ahigh glioblastoma cells contribute to resistance to anti-angiogenesis therapy through malformed vasculature., PMID:38658527
INHBA(+) cancer-associated fibroblasts generate an immunosuppressive tumor microenvironment in ovarian cancer., PMID:38360876
SYT7 (synaptotagmin 7) promotes cervical squamous cell carcinoma., PMID:38314285
DWN12088, A Prolyl-tRNA Synthetase Inhibitor, Alleviates Hepatic Injury in Nonalcoholic Steatohepatitis., PMID:38173372
Activin A marks a novel progenitor cell population during fracture healing and reveals a therapeutic strategy., PMID:38079220
Extracellular vesicles of iPS cells highly capable of producing HGF and TGF-β1 can attenuate Sjögren's syndrome via innate immunity regulation., PMID:37981065
TGF-β phospho antibody array identifies altered SMAD2, PI3K/AKT/SMAD, and RAC signaling contribute to the pathogenesis of myxomatous mitral valve disease., PMID:37908840
Radial glia promote microglial development through integrin αVβ8 -TGFβ1 signaling., PMID:37790363
Glutamine deficiency drives transforming growth factor-β signaling activation that gives rise to myofibroblastic carcinoma-associated fibroblasts., PMID:37706357
Lrig1-expression confers suppressive function to CD4+ cells and is essential for averting autoimmunity via the Smad2/3/Foxp3 axis., PMID:37666819
Atp6i deficient mouse model uncovers transforming growth factor-β1 /Smad2/3 as a key signaling pathway regulating odontoblast differentiation and tooth root formation., PMID:37599332
Ginsenosides in cancer: Targeting cell cycle arrest and apoptosis., PMID:37451663
Recruitment of regulatory T cells with rCCL17 promotes M2 microglia/macrophage polarization through TGFβ/TGFβR/Smad2/3 pathway in a mouse model of intracerebral hemorrhage., PMID:37257716
Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling., PMID:37052733
Multifunctional nanodrug performs sonodynamic therapy and inhibits TGF-β to boost immune response against colorectal cancer and liver metastasis., PMID:37037269
AMPK reduces macrophage endotoxin tolerance through inhibition of TGF-β1 production and its signaling pathway., PMID:37037116
CD109-regulated mechanical properties of endothelial cells., PMID:36929132
TGF-β is elevated in hyperuricemic individuals and mediates urate-induced hyperinflammatory phenotype in human mononuclear cells., PMID:36850003
CD44 mediates hyaluronan to promote the differentiation of human amniotic mesenchymal stem cells into chondrocytes., PMID:36680638
Combination therapy with DHA and BMSCs suppressed podocyte injury and attenuated renal fibrosis by modulating the TGF-β1/Smad pathway in MN mice., PMID:36648018
KIN17 promotes cell migration and invasion through stimulating the TGF-β/Smad2 pathway in hepatocellular carcinoma., PMID:36468848
Activin A directly impairs human cardiomyocyte contractile function indicating a potential role in heart failure development., PMID:36440002
Functional STAT3 Deficiency from Co-Localization with SMAD2/3 Can Account for Infective Predisposition and Hyper IgE-like Manifestation in Loeys-Dietz Syndrome., PMID:36350438
[New treatment for myelodysplastic syndromes: luspatercept and oral hypomethylating agents]., PMID:36198535
Inhibition of lysyl oxidase‑like 2 ameliorates folic acid‑induced renal tubulointerstitial fibrosis., PMID:36168418
Endothelin-1 Stimulates PAI-1 Protein Expression via Dual Transactivation Pathway Dependent ROCK and Phosphorylation of Smad2L., PMID:36093806
Macrophage-Derived TGF-β and VEGF Promote the Progression of Trauma-Induced Heterotopic Ossification., PMID:35986177
Combined treatment of xyloglucan derivative hydrogel and anti-C5a receptor antibody in preventing peritoneal adhesion., PMID:35944810
For research use only. Not for human or drug use.
