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Anti-Phospho-Smad3 (Ser425) Antibody (R3P95)

Catalog #:   RHF75507 Specific References (11) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC
Accession: P84022
Overview

Catalog No.

RHF75507

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:50-1:100

Target

Mad3, JV15-2, Mothers against decapentaplegic homolog 3, SMAD family member 3, Smad3, SMAD3, MADH3, hMAD-3, SMAD 3, MAD homolog 3, hSMAD3, Mothers against DPP homolog 3, Phospho-Smad3 (Ser425)

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P84022

Applications

IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3P95

Data Image
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human Skin Tissue using Phospho-Smad3 (Ser425) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Human tonsils using Phospho-Smad3 (Ser425) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
References

TGF-β pathway activation by idiopathic pulmonary fibrosis (IPF) fibroblast derived soluble factors is mediated by IL-6 trans-signaling., PMID:32070329

EPB41L5 Mediates TGFβ-Induced Metastasis of Gastric Cancer., PMID:30814110

Aberrant Smad3 phosphoisoforms in cyst-lining epithelial cells in the cpk mouse, a model of autosomal recessive polycystic kidney disease., PMID:28877884

Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-β., PMID:25707005

Regulation of chondroitin-4-sulfotransferase (CHST11) expression by opposing effects of arylsulfatase B on BMP4 and Wnt9A., PMID:25511584

Inhibition of histone deacetylase 2 mitigates profibrotic TGF-β1 responses in fibroblasts derived from Peyronie's plaque., PMID:23770939

Ligand-independent transforming growth factor-β type I receptor signalling mediates type I collagen-induced epithelial-mesenchymal transition., PMID:21984393

Role of increased penile expression of transforming growth factor-beta1 and activation of the Smad signaling pathway in erectile dysfunction in streptozotocin-induced diabetic rats., PMID:18778311

Hepatoprotective effect of Arazyme on CCl4-induced acute hepatic injury in SMP30 knock-out mice., PMID:18308447

The synthetic triterpenoid CDDO-imidazolide induces monocytic differentiation by activating the Smad and ERK signaling pathways in HL60 leukemia cells., PMID:16818503

Constitutive thrombospondin-1 overexpression contributes to autocrine transforming growth factor-beta signaling in cultured scleroderma fibroblasts., PMID:15855645

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Phospho-Smad3 (Ser425) Antibody (R3P95) [RHF75507]
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