Catalog No.
RHF75507
Species reactivity
Human, Mouse, Rat
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
IHC: 1:50-1:100
Target
Mad3, JV15-2, Mothers against decapentaplegic homolog 3, SMAD family member 3, Smad3, SMAD3, MADH3, hMAD-3, SMAD 3, MAD homolog 3, hSMAD3, Mothers against DPP homolog 3, Phospho-Smad3 (Ser425)
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P84022
Applications
IHC
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3P95
TGF-β pathway activation by idiopathic pulmonary fibrosis (IPF) fibroblast derived soluble factors is mediated by IL-6 trans-signaling., PMID:32070329
EPB41L5 Mediates TGFβ-Induced Metastasis of Gastric Cancer., PMID:30814110
Aberrant Smad3 phosphoisoforms in cyst-lining epithelial cells in the cpk mouse, a model of autosomal recessive polycystic kidney disease., PMID:28877884
Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-β., PMID:25707005
Regulation of chondroitin-4-sulfotransferase (CHST11) expression by opposing effects of arylsulfatase B on BMP4 and Wnt9A., PMID:25511584
Inhibition of histone deacetylase 2 mitigates profibrotic TGF-β1 responses in fibroblasts derived from Peyronie's plaque., PMID:23770939
Ligand-independent transforming growth factor-β type I receptor signalling mediates type I collagen-induced epithelial-mesenchymal transition., PMID:21984393
Role of increased penile expression of transforming growth factor-beta1 and activation of the Smad signaling pathway in erectile dysfunction in streptozotocin-induced diabetic rats., PMID:18778311
Hepatoprotective effect of Arazyme on CCl4-induced acute hepatic injury in SMP30 knock-out mice., PMID:18308447
The synthetic triterpenoid CDDO-imidazolide induces monocytic differentiation by activating the Smad and ERK signaling pathways in HL60 leukemia cells., PMID:16818503
Constitutive thrombospondin-1 overexpression contributes to autocrine transforming growth factor-beta signaling in cultured scleroderma fibroblasts., PMID:15855645
For research use only. Not for human or drug use.
