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Anti-NUCB2 Antibody (R3P88)

Catalog #:   RHF71201 Specific References (30) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: IHC, IP, WB
Accession: P80303
Overview

Catalog No.

RHF71201

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:100-1:200, IP: 1:100-1:200, WB: 1:500-1:1000

Target

NEFA, Gastric cancer antigen Zg4, Prepronesfatin, NUCB2, DNA-binding protein NEFA, Nucleobindin-2, Epididymis secretory protein Li 109

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P80303

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3P88

Data Image
  • Immunohistochemical
    IHC-P analysis of stomach tissue by NUCB2 antibody (RHF71201). IHC-P was performed using sections of the formalin-fixed paraffin-embedded stomach tissue;Result: Glandular cells are positively stained at the cytoplasm.
  • Immunoprecipitation
    Lane 1: HL-60 lysate ; Lane 2: NUCB2 immunoprecipitated from HL-60 lysate by RHF71201;Lane 3: The same as Lane 2 but use IgG isotype control antibody ;Result: RHF71201 can immunoprecipitate NUCB2;
  • Western blot
    Western blot analysis using NUCB2 mouse mAb against HL-60 lysate
References

"Satiety molecules" - nesfatin-1 and glucagon-like peptide 1 in blood serum in patients with anorexia nervosa and obesity., PMID:40331844

Immunohistochemical determination of the excitatory and inhibitory axonal endings contacting NUCB2/nesfatin-1 neurons., PMID:38157780

Chemogenetic activation of endogenous arginine vasopressin exerts anorexigenic effects via central nesfatin-1/NucB2 pathway., PMID:34134629

Central blockage of nesfatin-1 has anxiolytic effects but does not prevent corticotropin-releasing factor-induced anxiety in male rats., PMID:32736706

Endogenous NUCB2/Nesfatin-1 Regulates Energy Homeostasis Under Physiological Conditions in Male Rats., PMID:32722818

Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis., PMID:29475075

Nesfatin-1 influences the excitability of gastric distension-responsive neurons in the ventromedial hypothalamic nucleus of rats., PMID:27982684

The effects of Nigella sativa on thyroid function, serum Vascular Endothelial Growth Factor (VEGF) - 1, Nesfatin-1 and anthropometric features in patients with Hashimoto's thyroiditis: a randomized controlled trial., PMID:27852303

High NUCB2 expression level represents an independent negative prognostic factor in Chinese cohorts of non-metastatic clear cell renal cell carcinoma patients., PMID:27806328

Perianal Crohn's Disease is Associated with Distal Colonic Disease, Stricturing Disease Behavior, IBD-Associated Serologies and Genetic Variation in the JAK-STAT Pathway., PMID:26937622

Nucleobindin 1 (NUCB1) is a Golgi-resident marker of neurons., PMID:26666627

Nesfatin-1 signaling in the basom edial amygdala modulates the gastric distension-sensitive neurons discharge and decreases gastric motility via melanocortin 3/4 receptors and modified by the arcuate nucleus., PMID:26144374

Nesfatin-1 Suppresses Cardiac L-type Ca²⁺ Channels Through Melanocortin Type 4 Receptor and the Novel Protein Kinase C Theta Isoform Pathway., PMID:25997563

Effects of exogenous nesfatin-1 on gastric distention-sensitive neurons in the central nucleus of the amygdala and gastric motility in rats., PMID:25220707

Expression of nucleobindin 1 (NUCB1) in pancreatic islets and other endocrine tissues., PMID:25038744

A role of nesfatin-1/NucB2 in dehydration-induced anorexia., PMID:24829503

Nesfatin-1/nucleobindin-2 like immunoreactivity in the olfactory system, brain and pituitary of frog, Microhyla ornata., PMID:24768694

Central nesfatin-1 influences the excitability of ghrelin-responsive gastric distension neurons in the arcuate nucleus and reduces gastric motility in rats., PMID:24289827

Role of nesfatin-1 in a rat model of visceral hypersensitivity., PMID:23801843

Nesfatin-1 inhibits ovarian epithelial carcinoma cell proliferation in vitro., PMID:23764403

Central and peripheral expression and distribution of NUCB2/nesfatin-1., PMID:23537079

NEFA/nucleobindin-2 is a target autoantigen of the anti-Wa antibody and is associated with transfer RNA., PMID:22270345

Restraint stress alters the secretory activity of neurons co-expressing urocortin-1, cocaine- and amphetamine-regulated transcript peptide and nesfatin-1 in the mouse Edinger-Westphal nucleus., PMID:20043894

Molecular characterisation and expression analysis of SEREX-defined antigen NUCB2 in gastric epithelium, gastritis and gastric cancer., PMID:19351608

Molecular characterisation and expression analysis of SEREX-defined antigen NUCB2 in gastric epithelium, gastritis and gastric cancer., PMID:30256860

[Transfer RNA-associated anti-Wa antibody in patients with scleroderma and the target antigen NEFA/Nucleobindin-2]., PMID:17603255

Identification of nesfatin-1 as a satiety molecule in the hypothalamus., PMID:17036007

Serological identification and expression analysis of gastric cancer-associated genes., PMID:12087473

Golgi retention of human protein NEFA is mediated by its N-terminal Leu/Ile-rich region., PMID:11749975

Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein., PMID:7811391

Datasheet

Document Download

Anti-NUCB2 Antibody (R3P88).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-NUCB2 Antibody (R3P88) [RHF71201]
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