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Anti-Alpha Tubulin Antibody (R3P39)

Catalog #:   RHF62204 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: IHC, IP, WB
Accession: P68363, Q71U36
Overview

Catalog No.

RHF62204

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

IHC: 1:500, IP: 1:100-1:200, WB: 1:500-1:1000

Target

Tubulin alpha-ubiquitous chain, Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P68363, Q71U36

Applications

IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3P39

Data Image
  • Immunohistochemical
    IHC-P analysis of testis tissue by TUBA4A antibody (RHF62204). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue;Result: Cells in seminiferous ducts and Leydig cells are positively stained at cytoplasm and cell membrane.
  • Immunoprecipitation
    Lane 1: Hep G2 lysate; Lane 2: TUBA4A immunoprecipitated from Hep G2 lysate by RHF62204;Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: RHF62204 can immunoprecipitate TUBA4A;
  • Western blot
    Lane 1: 15 µg of A431 lysate; Lane 2: 15 µg of U-251MG lysate; Lane 3: 15 µg of HeLa lysate;Lane 4: 15 µg of Hep G2 lysate; Result: RHF62204 can detect α-Tubulin by Western blotting.
References

Targeting prostate cancer with site-specific antibody-drug conjugates enabled by tandemly fused ADP-ribosyl cyclases., PMID:40532576

Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector., PMID:40515423

Identifying Space-Resolved Proteins of the Murine Thymus, by Combining MALDI Mass Spectrometry Imaging and Proteomics., PMID:40502118

Production of functional recombinant antibodies in Dictyostelium discoideum., PMID:40468350

Albendazole specifically disrupts microtubules and protein turnover in the tegument of the cestode Mesocestoides corti., PMID:40465816

Dissection and Whole-Mount Immunofluorescent Staining of Mouse Hind Paw Muscles for Neuromuscular Junction Analysis., PMID:40432760

Mangrove-Crab Blood Cells Proliferate In Vitro and Display Neuronal Proteins Following Pituitary-Extract Stimulus., PMID:40415663

Evaluation of Outgrowth Potential of Rat Pheochromocytoma Cells Supplied with Highly Purified Rapidly Expanding Clones and Potential Application to Trigeminal Nerve Regeneration., PMID:40407612

Measuring Anti-aging Effects in Drosophila., PMID:40364980

Elucidating Critical Factors of Internalization and Drug Release of Antibody-Drug Conjugates (ADCs) Using Kinetic Parameters Evaluated by a Novel Tool Named TORCH., PMID:40326736

Orthogonal Site-Specific Dual Bioconjugation of Aryl and Alkyl Thiols., PMID:40323095

Red Light-Activated Reversible Inhibition of Protein Functions by Assembled Trap., PMID:40304578

Cx45 regulation by kinases and impact of expression in heart failure., PMID:40280467

Strategies to overcome resistance to enfortumab vedotin and pembrolizumab for patients with urothelial carcinoma: harnessing present knowledge for future advances., PMID:40225697

Engineering IgG antibodies for intracellular targeting and drug delivery., PMID:40222416

3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique., PMID:40200951

PayloadGenX, a multi-stage hybrid virtual screening approach for payload design: A microtubule inhibitor case study., PMID:40168837

Opportunities and Challenges in Antibody-Drug Conjugates for Cancer Therapy: A New Era for Cancer Treatment., PMID:40149295

Microtubules as a versatile reference standard for expansion microscopy., PMID:40140540

Multianalyte Nanopore Detection of Alzheimer's Biomarkers: A Label-Free Platform with Improved Sensitivity and Range., PMID:40129019

The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells., PMID:39982014

The Moonlighting Function of Glutaminase 2 Promotes Immune Evasion of Pancreatic Ductal Adenocarcinoma by Tubulin Tyrosine Ligase-like 1-Mediated Yes1 Associated Transcriptional Regulator Glutamylation., PMID:39924055

Research progress in bifunctional small molecules for cancer immunotherapy., PMID:39919914

Visualisation of Euglena gracilis organelles and cytoskeleton using expansion microscopy., PMID:39919801

Spatiotemporal distribution of Wnt signaling pathway markers in human congenital anomalies of kidney and urinary tract., PMID:39908631

Randomized Phase II Study of Bevacizumab with Weekly Anetumab Ravtansine or Weekly Paclitaxel in Platinum-Resistant/Refractory High-Grade Ovarian Cancer (NCI Trial)., PMID:39836408

Drug-Linker Constructs Bearing Unique Dual-Mechanism Tubulin Binding Payloads Tethered through Cleavable and Non-Cleavable Linkers., PMID:39801742

Identification and subcellular localization of the chaperonin NbCCTβ in Nosema bombycis., PMID:39800196

Histopathologic Comparison Among Drug Eruptions Induced by Enfortumab Vedotin, Brentuximab Vedotin, and Taxanes., PMID:39787333

Tumor-Targeted Cell-Penetrating Peptides Reveal That Monomethyl Auristatin E Temporally Modulates the Tumor Immune Microenvironment., PMID:39683778

Harvest of Vestibular End-Organs under Physiologic Conditions during Labyrinthectomy., PMID:39671345

Miniaturization and characterization of patient derived hepatocellular carcinoma tumor organoid cultures for cancer drug discovery applications., PMID:39662672

Functional investigation suggests CNTNAP5 involvement in glaucomatous neurodegeneration obtained from a GWAS in primary angle closure glaucoma., PMID:39637236

Progress in the discovery and development of anticancer agents from marine cyanobacteria., PMID:39620500

Synergistic inhibition of proliferation and induction of apoptosis in oral tongue squamous cell carcinoma by mebendazole and paclitaxel via PI3K/AKT pathway mitigation., PMID:39614899

Brentuximab-Induced Acute Interstitial Nephritis: A Case Report., PMID:39588062

Lymphocytic choriomeningitis arenavirus utilises intercellular connections for cell to cell spread., PMID:39578605

A New Method for Lateral Visualization of the Primary Cilia on the Surfaces of Cells Cultured on White Glass Rods., PMID:39556085

High Intraocular Concentration of Fibrinogen Regulates Retinal Function Via the ICAM-1 Pathway., PMID:39546292

Morphological aberration of corneal nerves in hyposecretory dry eye guinea pigs., PMID:39542915

Brain-targeted autoimmunity is strongly associated with Long COVID and its chronic fatigue syndrome as well as its affective symptoms., PMID:39522688

Utidelone plus pembrolizumab as the fourth-line combination treatment in non-small cell lung cancer with EGFR mutation: a case report., PMID:39423045

Highly Cytotoxic Cryptophycin Derivatives with Modification in Unit D for Conjugation., PMID:39324938

Collagen-V and K-α-1 Tubulin Antibodies as Potential Markers of Unsuspected GERD-Related Lung Damage: Insights from a Cross-Sectional Analysis., PMID:39317885

Pharmacovigilance study of the association between peripheral neuropathy and antibody-drug conjugates using the FDA adverse event reporting system., PMID:39271716

Noninvasive biomarkers for the detection of GERD-induced pulmonary injury., PMID:39266756

Comparative analysis of fixation techniques for signal detection in avian embryos., PMID:39245159

Enhancing antitumor efficacy of CLDN18.2-directed antibody-drug conjugates through autophagy inhibition in gastric cancer., PMID:39227365

Preclinical Characterization of ARX517, a Site-Specific Stable PSMA-Targeted Antibody-Drug Conjugate for the Treatment of Metastatic Castration-Resistant Prostate Cancer., PMID:39172730

Description of B abesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) from southern Florida, USA., PMID:39169986

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-Alpha Tubulin Antibody (R3P39) [RHF62204]
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