Catalog No.
RHF60202
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
eIF-5A, eIF-5A-1, EIF5A, eIF-4D, Rev-binding factor, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A-1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P63241
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3P31
Evaluation of a serum and urine-based ELISA testing distinct antigens for the diagnosis of tegumentary leishmaniasis., PMID:39952624
Development of a reliable, sensitive, and convenient assay for the discovery of new eIF5A hypusination inhibitors., PMID:39937781
Revealing disease subtypes and heterogeneity in common variable immunodeficiency through transcriptomic analysis., PMID:39396099
Novel deoxyhypusine synthase (DHPS) inhibitors target hypusination-induced vasculogenic mimicry (VM) against malignant melanoma., PMID:39393437
DHPS-Mediated Hypusination Regulates METTL3 Self-m6A-Methylation Modification to Promote Melanoma Proliferation and the Development of Novel Inhibitors., PMID:38952061
Oxidative Stress-Responsive Apoptosis Inducing Protein (ORAIP) Plays a Critical Role in Dextran Sulfate Sodium-Induced Murine Model of Ulcerative Colitis., PMID:38674185
Oxidative stress-responsive apoptosis inducing protein (ORAIP) plays a critical role in doxorubicin-induced apoptosis in rat cardiac myocytes., PMID:34864083
Dysregulation of Translation Factors EIF2S1, EIF5A and EIF6 in Intestinal-Type Adenocarcinoma (ITAC)., PMID:34830804
Eimeria tenella Translation Initiation Factor eIF-5A That Interacts With Calcium-Dependent Protein Kinase 4 Is Involved in Host Cell Invasion., PMID:33553005
Evaluation of a panel of tumor-associated antigens in breast cancer., PMID:31839604
Oxidative stress-responsive apoptosis inducing protein (ORAIP) plays a critical role in cerebral ischemia/reperfusion injury., PMID:31534168
eIF5A inhibition influences T cell dynamics in the pancreatic microenvironment of the humanized mouse model of Type 1 Diabetes., PMID:30733517
Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins., PMID:28852021
Bifunctional activity of deoxyhypusine synthase/hydroxylase from Trichomonas vaginalis., PMID:26410361
Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis., PMID:26348594
XPO1/CRM1 Inhibition Causes Antitumor Effects by Mitochondrial Accumulation of eIF5A., PMID:25878333
Blocking eIF5A modification in cervical cancer cells alters the expression of cancer-related genes and suppresses cell proliferation., PMID:24220243
Characterization of a novel polyclonal anti-hypusine antibody., PMID:24024105
Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine and tyrosine and O-glycosylated in Trichomonas vaginalis., PMID:22212642
Identification of two novel Trichomonas vaginalis eif-5a genes., PMID:20060503
Insights on eukaryotic translation initiation factor 5A (eIF5A) in the brain and aging., PMID:18606156
Global analysis of gel mobility of proteins and its use in target identification., PMID:18292091
The N-terminal region of eukaryotic translation initiation factor 5A signals to nuclear localization of the protein., PMID:17707773
Identification of a ligand for IgG-Fc derived from a soluble peptide library based on fusion proteins secreted by S. cerevisiae., PMID:17492714
Inhibition of multidrug-resistant HIV-1 by interference with cellular S-adenosylmethionine decarboxylase activity., PMID:16941339
Eukaryotic initiation factor 5A-1 (eIF5A-1) as a diagnostic marker for aberrant proliferation in intraepithelial neoplasia of the vulva., PMID:15262146
Higher activity of recombinant bovine deoxyhypusine synthase vs. human deoxyhypusine synthase., PMID:15039063
The Caenorhabditis elegans eukaryotic initiation factor 5A homologue, IFF-1, is required for germ cell proliferation, gametogenesis and localization of the P-granule component PGL-1., PMID:15003625
Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and green fluorescent protein tagging., PMID:12210765
Protein expression strategies for identification of novel target proteins., PMID:10803608
Inhibition of CD83 cell surface expression during dendritic cell maturation by interference with nuclear export of CD83 mRNA., PMID:10790432
Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1., PMID:10381392
Interaction of the HIV-1 rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5., PMID:9465063
Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta., PMID:9405152
Biochemical and immunological characterization of deoxyhypusine synthase purified from the yeast Saccharomyces carlsbergensis., PMID:9163530
The subcellular distribution of eukaryotic translation initiation factor, eIF-5A, in cultured cells., PMID:8660923
Deoxyhypusine synthase from rat testis: purification and characterization., PMID:7721768
Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs., PMID:8057425
Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine in Saccharomyces cerevisiae., PMID:8325852
Detection of the hypusine-containing protein (HP = eIF-5A) in crude yeast extracts by two-dimensional western blots., PMID:1281091
Differential expression of genes encoding the hypusine-containing translation initiation factor, eIF-5A, in tobacco., PMID:1542563
For research use only. Not for human or drug use.
